Genome-Wide Identification and Expression Analysis of the <i>Aux/IAA</i> Gene Family of the Drumstick Tree (<i>Moringa oleifera</i> Lam.) Reveals Regulatory Effects on Shoot Regeneration
Auxin plays a critical role in organogenesis in plants. The classical auxin signaling pathway holds that auxin initiates downstream signal transduction by degrading Aux/IAA transcription repressors that interact with ARF transcription factors. In this study, 23 <i>MoIAA</i> genes were id...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/24/15729 |
| Summary: | Auxin plays a critical role in organogenesis in plants. The classical auxin signaling pathway holds that auxin initiates downstream signal transduction by degrading Aux/IAA transcription repressors that interact with ARF transcription factors. In this study, 23 <i>MoIAA</i> genes were identified in the drumstick tree genome. All <i>MoIAA</i> genes were located within five subfamilies based on phylogenetic evolution analysis; the gene characteristics and promoter cis-elements were also analyzed. The protein interaction network between the MoIAAs with MoARFs was complex. The <i>MoIAA</i> gene family responded positively to NAA treatment, exhibiting different patterns and degrees, notably for <i>MoIAA1</i>, <i>MoIAA7</i> and <i>MoIAA13</i>. The three genes expressed and functioned in the nucleus; only the intact encoding protein of <i>MoIAA13</i> exhibited transcriptional activation activity. The shoot regeneration capacity in the <i>35S::MoIAA13-OE</i> transgenic line was considerably lower than in the wild type. These results establish a foundation for further research on <i>MoIAA</i> gene function and provide useful information for improved tissue culture efficiency and molecular breeding of <i>M. oleifera</i>. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |