Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody
Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based na...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/1/111 |
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| author | Yu-Wei Lin Hung-Cheng Su Emmanuel Naveen Raj Kuang-Kai Liu Chien-Jen Chang Tzu-Chia Hsu Po-Yun Cheng Rou-Hsin Wang Yen-Her Lai Chien-Hung Chen Yen-Cheng Lin Jui-I Chao |
| author_facet | Yu-Wei Lin Hung-Cheng Su Emmanuel Naveen Raj Kuang-Kai Liu Chien-Jen Chang Tzu-Chia Hsu Po-Yun Cheng Rou-Hsin Wang Yen-Her Lai Chien-Hung Chen Yen-Cheng Lin Jui-I Chao |
| author_sort | Yu-Wei Lin |
| collection | DOAJ |
| description | Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy. |
| first_indexed | 2024-03-09T11:27:12Z |
| format | Article |
| id | doaj.art-174683a338e84a5788c13d3eecfa23ae |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T11:27:12Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-174683a338e84a5788c13d3eecfa23ae2023-11-30T23:57:49ZengMDPI AGPharmaceutics1999-49232022-12-0115111110.3390/pharmaceutics15010111Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR AntibodyYu-Wei Lin0Hung-Cheng Su1Emmanuel Naveen Raj2Kuang-Kai Liu3Chien-Jen Chang4Tzu-Chia Hsu5Po-Yun Cheng6Rou-Hsin Wang7Yen-Her Lai8Chien-Hung Chen9Yen-Cheng Lin10Jui-I Chao11Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanInstitute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanInstitute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, TaiwanNanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy.https://www.mdpi.com/1999-4923/15/1/111nanodiamondcetuximablung cancer therapyEGFRtumorigenesis |
| spellingShingle | Yu-Wei Lin Hung-Cheng Su Emmanuel Naveen Raj Kuang-Kai Liu Chien-Jen Chang Tzu-Chia Hsu Po-Yun Cheng Rou-Hsin Wang Yen-Her Lai Chien-Hung Chen Yen-Cheng Lin Jui-I Chao Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody Pharmaceutics nanodiamond cetuximab lung cancer therapy EGFR tumorigenesis |
| title | Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody |
| title_full | Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody |
| title_fullStr | Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody |
| title_full_unstemmed | Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody |
| title_short | Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody |
| title_sort | targeting egfr and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo using nanodiamond conjugated specific egfr antibody |
| topic | nanodiamond cetuximab lung cancer therapy EGFR tumorigenesis |
| url | https://www.mdpi.com/1999-4923/15/1/111 |
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