Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4
<p>Trophoblast participates in preventing allorecognition and controlling pathogens that compromise fetal wellbeing. Toll-like receptors recognize conserved sequences on the pathogens surface and trigger effector cell functions. Porphyromonas gingivalis is thought to spread to the umbilical co...
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| Format: | Article |
| Language: | English |
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Faculty of Dentistry, Universitas Indonesia
2013-09-01
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| Series: | Journal of Dentistry Indonesia |
| Subjects: | |
| Online Access: | http://jdentistry.ui.ac.id/index.php/JDI/article/view/150 |
| _version_ | 1811304705407582208 |
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| author | Banun Kusumawardani Marsetyawan HNE Soesatyo Djaswadi Dasuki Widya Asmara |
| author_facet | Banun Kusumawardani Marsetyawan HNE Soesatyo Djaswadi Dasuki Widya Asmara |
| author_sort | Banun Kusumawardani |
| collection | DOAJ |
| description | <p>Trophoblast participates in preventing allorecognition and controlling pathogens that compromise fetal wellbeing. Toll-like receptors recognize conserved sequences on the pathogens surface and trigger effector cell functions. Porphyromonas gingivalis is thought to spread to the umbilical cord and cause fetal growth restriction. <strong>Objective:</strong> To characterize expression and function of TLR-2 and TLR-4 in trophoblast cells from Porphyromonas gingivalisinfected pregnant rats. <strong>Methods:</strong> Live Porphyromonas gingivalis were challenged into the maxillary first molar subgingival sulcus of female rats before and/or during pregnancy and sacrified on gestational day (GD) 14 and 20. Porphyromonas gingivalis was detected by API-ZYM system in the maternal blood of the retro-orbital venous plexus and the umbilical cord. TLR-2 and TLR-4 expressions in trophoblast cells was detected by immunohistochemistry. <strong>Results:</strong> Porphyromonas gingivalis was first detected in the maternal blood and finally spread to the umbilical cord. Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell in treated groups had significantly higher expression of TLR-2 and TLR-4 than control group (p<0.05).<strong> Conclusion: </strong>Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell are able to recognize Porphyromonas gingivalis through TLR-2 and TLR-4 expression. The ligation of TLR-2 and TLR-4 promoted cytokine production and induced trophoblast cell death. These findings strengthen links between periodontal disease and fetal growth restriction.</p><p>DOI: 10.14693/jdi.v20i2.150</p> |
| first_indexed | 2024-04-13T08:12:07Z |
| format | Article |
| id | doaj.art-1746f8228fe84296a12997cff50cc075 |
| institution | Directory Open Access Journal |
| issn | 1693-9697 2355-4800 |
| language | English |
| last_indexed | 2024-04-13T08:12:07Z |
| publishDate | 2013-09-01 |
| publisher | Faculty of Dentistry, Universitas Indonesia |
| record_format | Article |
| series | Journal of Dentistry Indonesia |
| spelling | doaj.art-1746f8228fe84296a12997cff50cc0752022-12-22T02:54:54ZengFaculty of Dentistry, Universitas IndonesiaJournal of Dentistry Indonesia1693-96972355-48002013-09-01202323810.14693/jdi.v20i2.150142Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4Banun Kusumawardani0Marsetyawan HNE Soesatyo1Djaswadi Dasuki2Widya Asmara3Postgraduate Program of Medical and Health Science, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta 55281Department of Histology and Cell Biology Faculty of Medicine Universitas Gadjah Mada Yogyakarta 55281Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta 55281Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281<p>Trophoblast participates in preventing allorecognition and controlling pathogens that compromise fetal wellbeing. Toll-like receptors recognize conserved sequences on the pathogens surface and trigger effector cell functions. Porphyromonas gingivalis is thought to spread to the umbilical cord and cause fetal growth restriction. <strong>Objective:</strong> To characterize expression and function of TLR-2 and TLR-4 in trophoblast cells from Porphyromonas gingivalisinfected pregnant rats. <strong>Methods:</strong> Live Porphyromonas gingivalis were challenged into the maxillary first molar subgingival sulcus of female rats before and/or during pregnancy and sacrified on gestational day (GD) 14 and 20. Porphyromonas gingivalis was detected by API-ZYM system in the maternal blood of the retro-orbital venous plexus and the umbilical cord. TLR-2 and TLR-4 expressions in trophoblast cells was detected by immunohistochemistry. <strong>Results:</strong> Porphyromonas gingivalis was first detected in the maternal blood and finally spread to the umbilical cord. Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell in treated groups had significantly higher expression of TLR-2 and TLR-4 than control group (p<0.05).<strong> Conclusion: </strong>Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell are able to recognize Porphyromonas gingivalis through TLR-2 and TLR-4 expression. The ligation of TLR-2 and TLR-4 promoted cytokine production and induced trophoblast cell death. These findings strengthen links between periodontal disease and fetal growth restriction.</p><p>DOI: 10.14693/jdi.v20i2.150</p>http://jdentistry.ui.ac.id/index.php/JDI/article/view/150bacteremiafetal growth restrictionperiodontitisPorphyromonas gingivalistoll-like receptors |
| spellingShingle | Banun Kusumawardani Marsetyawan HNE Soesatyo Djaswadi Dasuki Widya Asmara Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 Journal of Dentistry Indonesia bacteremia fetal growth restriction periodontitis Porphyromonas gingivalis toll-like receptors |
| title | Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 |
| title_full | Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 |
| title_fullStr | Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 |
| title_full_unstemmed | Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 |
| title_short | Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 |
| title_sort | placental trophoblast responses to porphyromonas gingivalis mediated by toll like receptor 2 and 4 |
| topic | bacteremia fetal growth restriction periodontitis Porphyromonas gingivalis toll-like receptors |
| url | http://jdentistry.ui.ac.id/index.php/JDI/article/view/150 |
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