Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography
Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) wit...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-04-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177913001330 |
| _version_ | 1818736735258935296 |
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| author | Lei-Wen Xiang Jing Li Jin-Ming Lin Hai-Fang Li |
| author_facet | Lei-Wen Xiang Jing Li Jin-Ming Lin Hai-Fang Li |
| author_sort | Lei-Wen Xiang |
| collection | DOAJ |
| description | Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 (5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957â0.9993. The proposed analytical method has satisfactory repeatability (the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49â97.90%, 95.38â96.45%, 112.46â115.78% and 90.82â97.13% with standard deviation of <5%, respectively) and the limits of detection (LODs, S/Nâ¥3) for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis. Keywords: Gouty arthritis, Creatinine, Uric acid, Hypoxanthine, Xanthine, High-performance liquid chromatography |
| first_indexed | 2024-12-18T00:41:52Z |
| format | Article |
| id | doaj.art-17475255540343ec949cb874947bec75 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-18T00:41:52Z |
| publishDate | 2014-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-17475255540343ec949cb874947bec752022-12-21T21:26:52ZengElsevierJournal of Pharmaceutical Analysis2095-17792014-04-0142153158Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatographyLei-Wen Xiang0Jing Li1Jin-Ming Lin2Hai-Fang Li3Beijing Key Laboratory of Microanalysis Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China; Institute of Food and Fermentation Industries, Fuqing Branch of Fujian Normal University, Fuqing 350300, ChinaBeijing Key Laboratory of Microanalysis Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, ChinaBeijing Key Laboratory of Microanalysis Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, ChinaBeijing Key Laboratory of Microanalysis Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China; Corresponding author. Tel./fax: +86 10 62797463.Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 (5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957â0.9993. The proposed analytical method has satisfactory repeatability (the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49â97.90%, 95.38â96.45%, 112.46â115.78% and 90.82â97.13% with standard deviation of <5%, respectively) and the limits of detection (LODs, S/Nâ¥3) for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis. Keywords: Gouty arthritis, Creatinine, Uric acid, Hypoxanthine, Xanthine, High-performance liquid chromatographyhttp://www.sciencedirect.com/science/article/pii/S2095177913001330 |
| spellingShingle | Lei-Wen Xiang Jing Li Jin-Ming Lin Hai-Fang Li Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography Journal of Pharmaceutical Analysis |
| title | Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography |
| title_full | Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography |
| title_fullStr | Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography |
| title_full_unstemmed | Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography |
| title_short | Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography |
| title_sort | determination of gouty arthritis biomarkers in human urine using reversed phase high performance liquid chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S2095177913001330 |
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