Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management
(1) Background: Soil salinity impacts plant growth and productivity in steppic regions. Thus, the current study aims at producing, at low cost, an inoculum composed of native arbuscular mycorrhizal fungi (AMF) strains adapted to saline soil management. (2) Methods: The propagation of the native AMF...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2673-7140/3/2/30 |
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| author | Karima Bencherif Frederic Laruelle Benoit Tisserant Yolande Dalpé Anissa Lounés-Hadj Sahraoui |
| author_facet | Karima Bencherif Frederic Laruelle Benoit Tisserant Yolande Dalpé Anissa Lounés-Hadj Sahraoui |
| author_sort | Karima Bencherif |
| collection | DOAJ |
| description | (1) Background: Soil salinity impacts plant growth and productivity in steppic regions. Thus, the current study aims at producing, at low cost, an inoculum composed of native arbuscular mycorrhizal fungi (AMF) strains adapted to saline soil management. (2) Methods: The propagation of the native AMF strains was carried out in three natural saline soils presenting increasing salinity levels (4.5, 8.5 and 9.3 dS·m<sup>−1</sup> in Boughzoul (BG), Salt Rocket (SR) and Zaafrane (ZA) sites, respectively). Three host plant species (alfalfa, clover and leek) were tested as trap cultures. AMF spore richness and diversity, the quantification of soil microbial biomass and the determination of the most probable number (MPN) were carried out before and after 24 months culture. Moreover, the mycorrhizal rates of the host plant species were assessed. (3) Results: The moderate saline soil (SR) planted with alfalfa was found to be the most suitable for AMF inoculum production with a maximum of 650 spores, 10 g<sup>−1</sup> of soil, a mycorrhizal rate of 86%, 70 propagules·g<sup>−1</sup> of soil and the highest microbial biomass content. (4) Conclusion: This study points out the best combination for the production of a more adapted AMF biofertilizer for saline soil management and proposes several indicators for biofertilizer quality evaluation. |
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| last_indexed | 2024-03-11T01:55:41Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-1748aa48a9f24d7ba21c4c4e0b8552da2023-11-18T12:39:25ZengMDPI AGStresses2673-71402023-04-013240442310.3390/stresses3020030Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil ManagementKarima Bencherif0Frederic Laruelle1Benoit Tisserant2Yolande Dalpé3Anissa Lounés-Hadj Sahraoui4Départements des Sciences Agrovétérinaires, Faculté des Sciences de la Nature et de la Vie, Université de Djelfa, Route de Moudjbara, Boite Postale 3117, Djelfa 17000, AlgeriaUnité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d’Opale, UR 4492, SFR Condorcet FR CNRS 3417, 50 Rue Ferdinand Buisson, 62228 Calais cedex, FranceUnité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d’Opale, UR 4492, SFR Condorcet FR CNRS 3417, 50 Rue Ferdinand Buisson, 62228 Calais cedex, FranceCentre de Recherche et Développement d’Ottawa, Agriculture et Agroalimentaire Canada, 960 Carling Ave., Ottawa, ON K1A 0C6, CanadaUnité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d’Opale, UR 4492, SFR Condorcet FR CNRS 3417, 50 Rue Ferdinand Buisson, 62228 Calais cedex, France(1) Background: Soil salinity impacts plant growth and productivity in steppic regions. Thus, the current study aims at producing, at low cost, an inoculum composed of native arbuscular mycorrhizal fungi (AMF) strains adapted to saline soil management. (2) Methods: The propagation of the native AMF strains was carried out in three natural saline soils presenting increasing salinity levels (4.5, 8.5 and 9.3 dS·m<sup>−1</sup> in Boughzoul (BG), Salt Rocket (SR) and Zaafrane (ZA) sites, respectively). Three host plant species (alfalfa, clover and leek) were tested as trap cultures. AMF spore richness and diversity, the quantification of soil microbial biomass and the determination of the most probable number (MPN) were carried out before and after 24 months culture. Moreover, the mycorrhizal rates of the host plant species were assessed. (3) Results: The moderate saline soil (SR) planted with alfalfa was found to be the most suitable for AMF inoculum production with a maximum of 650 spores, 10 g<sup>−1</sup> of soil, a mycorrhizal rate of 86%, 70 propagules·g<sup>−1</sup> of soil and the highest microbial biomass content. (4) Conclusion: This study points out the best combination for the production of a more adapted AMF biofertilizer for saline soil management and proposes several indicators for biofertilizer quality evaluation.https://www.mdpi.com/2673-7140/3/2/30biofertilizerAMF inoculumsalinityhost plant |
| spellingShingle | Karima Bencherif Frederic Laruelle Benoit Tisserant Yolande Dalpé Anissa Lounés-Hadj Sahraoui Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management Stresses biofertilizer AMF inoculum salinity host plant |
| title | Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management |
| title_full | Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management |
| title_fullStr | Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management |
| title_full_unstemmed | Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management |
| title_short | Engineering Approach for Production of Arbuscular Mycorrhizal Inoculum Adapted to Saline Soil Management |
| title_sort | engineering approach for production of arbuscular mycorrhizal inoculum adapted to saline soil management |
| topic | biofertilizer AMF inoculum salinity host plant |
| url | https://www.mdpi.com/2673-7140/3/2/30 |
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