Antimicrobial potential of Casearia sylvestris against oral bacteria
Abstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For t...
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| Format: | Article |
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Universidade Estadual Paulista
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| Series: | Revista de Odontologia da UNESP |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400214&lng=en&tlng=en |
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| author | Amanda Henriques CAVALHEIRO Ana Paula Terossi GODOI Tatiane Cruz de CARVALHO Alma Blasida CATIRSE Niege Araçari Jacometti Cardoso FURTADO |
| author_facet | Amanda Henriques CAVALHEIRO Ana Paula Terossi GODOI Tatiane Cruz de CARVALHO Alma Blasida CATIRSE Niege Araçari Jacometti Cardoso FURTADO |
| author_sort | Amanda Henriques CAVALHEIRO |
| collection | DOAJ |
| description | Abstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result All extracts evaluated in the used protocol displayed MIC values higher than 400 µg/mL and few showed bactericidal activity. The antimicrobial activity of essential oil was higher than the activity of the extracts, and the best minimum inhibitory concentration and minimum bactericidal concentration values were obtained against L. casei (MIC of 0.023 µg/mL and MBC of 0.046 µg/mL) and S. mutans (MIC of 25 µg/mL and MBC of 50 µg/mL), respectively. Conclusion The essential oil of Casearia sylvestris has significant antimicrobial activity against oral microorganisms. |
| first_indexed | 2024-12-13T16:41:34Z |
| format | Article |
| id | doaj.art-174995549efa4ad7beccac9ba68be271 |
| institution | Directory Open Access Journal |
| issn | 1807-2577 |
| language | English |
| last_indexed | 2024-12-13T16:41:34Z |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Revista de Odontologia da UNESP |
| spelling | doaj.art-174995549efa4ad7beccac9ba68be2712022-12-21T23:38:16ZengUniversidade Estadual PaulistaRevista de Odontologia da UNESP1807-257745421421810.1590/1807-2577.11015S1807-25772016000400214Antimicrobial potential of Casearia sylvestris against oral bacteriaAmanda Henriques CAVALHEIROAna Paula Terossi GODOITatiane Cruz de CARVALHOAlma Blasida CATIRSENiege Araçari Jacometti Cardoso FURTADOAbstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result All extracts evaluated in the used protocol displayed MIC values higher than 400 µg/mL and few showed bactericidal activity. The antimicrobial activity of essential oil was higher than the activity of the extracts, and the best minimum inhibitory concentration and minimum bactericidal concentration values were obtained against L. casei (MIC of 0.023 µg/mL and MBC of 0.046 µg/mL) and S. mutans (MIC of 25 µg/mL and MBC of 50 µg/mL), respectively. Conclusion The essential oil of Casearia sylvestris has significant antimicrobial activity against oral microorganisms.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400214&lng=en&tlng=enLaboratory researchdental cariesmicroorganismsphytotherapy |
| spellingShingle | Amanda Henriques CAVALHEIRO Ana Paula Terossi GODOI Tatiane Cruz de CARVALHO Alma Blasida CATIRSE Niege Araçari Jacometti Cardoso FURTADO Antimicrobial potential of Casearia sylvestris against oral bacteria Revista de Odontologia da UNESP Laboratory research dental caries microorganisms phytotherapy |
| title | Antimicrobial potential of Casearia sylvestris against oral bacteria |
| title_full | Antimicrobial potential of Casearia sylvestris against oral bacteria |
| title_fullStr | Antimicrobial potential of Casearia sylvestris against oral bacteria |
| title_full_unstemmed | Antimicrobial potential of Casearia sylvestris against oral bacteria |
| title_short | Antimicrobial potential of Casearia sylvestris against oral bacteria |
| title_sort | antimicrobial potential of casearia sylvestris against oral bacteria |
| topic | Laboratory research dental caries microorganisms phytotherapy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400214&lng=en&tlng=en |
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