The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study
Background: Despite their lower strength, glass ionomer cements (GICs) are widely used as restorative materials because of their anti-cariogenic properties, direct adhesion to tooth structure and good biocompatibility. Recently, the addition of nano-hydroxyapatite (nano-HA)-silica to conventional GI...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Contemporary Clinical Dentistry |
| Subjects: | |
| Online Access: | http://www.contempclindent.org/article.asp?issn=0976-237X;year=2019;volume=10;issue=2;spage=324;epage=332;aulast=Hii |
| _version_ | 1818246941550575616 |
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| author | Siew Ching Hii Norhayati Luddin Thirumulu Ponnuraj Kannan Ismail Ab Rahman Nik Rozainah Nik Abdul Ghani |
| author_facet | Siew Ching Hii Norhayati Luddin Thirumulu Ponnuraj Kannan Ismail Ab Rahman Nik Rozainah Nik Abdul Ghani |
| author_sort | Siew Ching Hii |
| collection | DOAJ |
| description | Background: Despite their lower strength, glass ionomer cements (GICs) are widely used as restorative materials because of their anti-cariogenic properties, direct adhesion to tooth structure and good biocompatibility. Recently, the addition of nano-hydroxyapatite (nano-HA)-silica to conventional GIC (cGIC) has been shown to improve the strength of cGIC. However, the biocompatibility and cell attachment properties of this material are unknown. Aims: This study aims to evaluate and compare the cytotoxicity and cell attachment properties of cGIC and nano-HA-silica-GIC on dental pulp stem cells (DPSCs). Methods and Materials: Material extracts of nano-HA-silica-GIC and cGIC were prepared into seven serial dilutions and applied to 96 well plates seeded with DPSCs. After 72 h, the cell viability was determined using MTT assay. The DPSCs cell attachment properties were examined under scanning electron microscope (SEM) after 24 and 72 h. Kruskal–Wallis test was used to analyse the data for MTT assay (P < 0.05). SEM images of cell attachment properties were also described. Results: Nano-HA-silica-GIC and cGIC was shown to be slight to non-cytotoxic at all concentrations, except 200 mg/ml. Moderate cytotoxicity has been observed at 200 mg/ml concentration where nano-HA-silica-GIC and cGIC revealed cell viability values of 44.38 and 42.15%, respectively. Nano-HA-silica-GIC demonstrated better cell viability values than cGIC at all concentrations except for 6.25 and 12.5 mg/ml. Nevertheless, the results were not statistically significant (P > 0.05). SEM examination revealed the increasing numbers of DPSCs attached to both groups with prominent filopodia, especially after 72 h. Conclusions: Nano-HA-silica-GIC exhibited good biocompatibility which is comparable to cGIC and favoured the attachment of DPSCs. |
| first_indexed | 2024-12-12T14:56:49Z |
| format | Article |
| id | doaj.art-174a8badded2463bb0298fc047dee465 |
| institution | Directory Open Access Journal |
| issn | 0976-237X 0976-2361 |
| language | English |
| last_indexed | 2024-12-12T14:56:49Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Contemporary Clinical Dentistry |
| spelling | doaj.art-174a8badded2463bb0298fc047dee4652022-12-22T00:20:53ZengWolters Kluwer Medknow PublicationsContemporary Clinical Dentistry0976-237X0976-23612019-01-0110232433210.4103/ccd.ccd_581_18The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative studySiew Ching HiiNorhayati LuddinThirumulu Ponnuraj KannanIsmail Ab RahmanNik Rozainah Nik Abdul GhaniBackground: Despite their lower strength, glass ionomer cements (GICs) are widely used as restorative materials because of their anti-cariogenic properties, direct adhesion to tooth structure and good biocompatibility. Recently, the addition of nano-hydroxyapatite (nano-HA)-silica to conventional GIC (cGIC) has been shown to improve the strength of cGIC. However, the biocompatibility and cell attachment properties of this material are unknown. Aims: This study aims to evaluate and compare the cytotoxicity and cell attachment properties of cGIC and nano-HA-silica-GIC on dental pulp stem cells (DPSCs). Methods and Materials: Material extracts of nano-HA-silica-GIC and cGIC were prepared into seven serial dilutions and applied to 96 well plates seeded with DPSCs. After 72 h, the cell viability was determined using MTT assay. The DPSCs cell attachment properties were examined under scanning electron microscope (SEM) after 24 and 72 h. Kruskal–Wallis test was used to analyse the data for MTT assay (P < 0.05). SEM images of cell attachment properties were also described. Results: Nano-HA-silica-GIC and cGIC was shown to be slight to non-cytotoxic at all concentrations, except 200 mg/ml. Moderate cytotoxicity has been observed at 200 mg/ml concentration where nano-HA-silica-GIC and cGIC revealed cell viability values of 44.38 and 42.15%, respectively. Nano-HA-silica-GIC demonstrated better cell viability values than cGIC at all concentrations except for 6.25 and 12.5 mg/ml. Nevertheless, the results were not statistically significant (P > 0.05). SEM examination revealed the increasing numbers of DPSCs attached to both groups with prominent filopodia, especially after 72 h. Conclusions: Nano-HA-silica-GIC exhibited good biocompatibility which is comparable to cGIC and favoured the attachment of DPSCs.http://www.contempclindent.org/article.asp?issn=0976-237X;year=2019;volume=10;issue=2;spage=324;epage=332;aulast=Hiicell attachmentcytotoxicitydental pulpglass ionomer cementsstem cells |
| spellingShingle | Siew Ching Hii Norhayati Luddin Thirumulu Ponnuraj Kannan Ismail Ab Rahman Nik Rozainah Nik Abdul Ghani The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study Contemporary Clinical Dentistry cell attachment cytotoxicity dental pulp glass ionomer cements stem cells |
| title | The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study |
| title_full | The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study |
| title_fullStr | The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study |
| title_full_unstemmed | The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study |
| title_short | The biological evaluation of conventional and nano-hydroxyapatite-silica glass ionomer cement on dental pulp stem cells: A comparative study |
| title_sort | biological evaluation of conventional and nano hydroxyapatite silica glass ionomer cement on dental pulp stem cells a comparative study |
| topic | cell attachment cytotoxicity dental pulp glass ionomer cements stem cells |
| url | http://www.contempclindent.org/article.asp?issn=0976-237X;year=2019;volume=10;issue=2;spage=324;epage=332;aulast=Hii |
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