Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine
Molecular imprinting is an efficient strategy to make the detection of compounds more specific and more selective. This targeted analytical strategy using molecularly imprinted polymer (MIP) synthesis needs to obtain the optimized conditions. A selective molecularly imprinted polymer was prepared fo...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/8/1660 |
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| author | Marie Elhachem Elias Bou-Maroun Maher Abboud Philippe Cayot Richard G. Maroun |
| author_facet | Marie Elhachem Elias Bou-Maroun Maher Abboud Philippe Cayot Richard G. Maroun |
| author_sort | Marie Elhachem |
| collection | DOAJ |
| description | Molecular imprinting is an efficient strategy to make the detection of compounds more specific and more selective. This targeted analytical strategy using molecularly imprinted polymer (MIP) synthesis needs to obtain the optimized conditions. A selective molecularly imprinted polymer was prepared for caffeic acid (CA) detection after varying the following synthesis parameters: functional monomer type (N-phenylacrylamide, N-PAA or methacrylic acid, MAA), solvent type (acetonitrile/methanol or acetonitrile/toluene), and the polymerization method (UV or thermal initiation). The optimal polymer was obtained using MAA as a functional monomer, acetonitrile/methanol as solvent, and UV polymerization. Morphological characterizations were done for the optimal CA-MIP using mid-infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption. The optimal polymer showed good specificity and selectivity in the presence of interferents (antioxidants having a chemical structure close to CA) in a hydroalcoholic solution. The electrochemical detection of CA was performed by cyclic voltammetry (CV) after the interaction between CA and the optimal MIP in a wine sample. The linear range of the developed method was between 0 and 1.11 mM, the limit of detection (LOD) was 0.13 mM, and the limit of quantification (LOQ) was 0.32 mM. HPLC-UV was used to validate the newly developed method. Recovery values were between 104% and 111%. |
| first_indexed | 2024-03-11T05:00:49Z |
| format | Article |
| id | doaj.art-174c9153c2b9462ea2e44172b0d84171 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:00:49Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-174c9153c2b9462ea2e44172b0d841712023-11-17T19:14:52ZengMDPI AGFoods2304-81582023-04-01128166010.3390/foods12081660Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in WineMarie Elhachem0Elias Bou-Maroun1Maher Abboud2Philippe Cayot3Richard G. Maroun4PAM UMR A 02.102, Institut Agro, Université Bourgogne Franche-Comté, 1 Esplanade Erasme, F-21000 Dijon, FrancePAM UMR A 02.102, Institut Agro, Université Bourgogne Franche-Comté, 1 Esplanade Erasme, F-21000 Dijon, FranceUEGP Unité Environnement, Génomique et Protéomique, Faculty of Sciences, Saint Joseph University, BP 17-5208 Mar Mikhael, Beirut 1104 2020, LebanonPAM UMR A 02.102, Institut Agro, Université Bourgogne Franche-Comté, 1 Esplanade Erasme, F-21000 Dijon, FranceCentre d’Analyses et de Recherche, Laboratoire CTA, UR TVA, Faculty of Sciences, Saint Joseph University, Beirut 1104 2020, LebanonMolecular imprinting is an efficient strategy to make the detection of compounds more specific and more selective. This targeted analytical strategy using molecularly imprinted polymer (MIP) synthesis needs to obtain the optimized conditions. A selective molecularly imprinted polymer was prepared for caffeic acid (CA) detection after varying the following synthesis parameters: functional monomer type (N-phenylacrylamide, N-PAA or methacrylic acid, MAA), solvent type (acetonitrile/methanol or acetonitrile/toluene), and the polymerization method (UV or thermal initiation). The optimal polymer was obtained using MAA as a functional monomer, acetonitrile/methanol as solvent, and UV polymerization. Morphological characterizations were done for the optimal CA-MIP using mid-infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption. The optimal polymer showed good specificity and selectivity in the presence of interferents (antioxidants having a chemical structure close to CA) in a hydroalcoholic solution. The electrochemical detection of CA was performed by cyclic voltammetry (CV) after the interaction between CA and the optimal MIP in a wine sample. The linear range of the developed method was between 0 and 1.11 mM, the limit of detection (LOD) was 0.13 mM, and the limit of quantification (LOQ) was 0.32 mM. HPLC-UV was used to validate the newly developed method. Recovery values were between 104% and 111%.https://www.mdpi.com/2304-8158/12/8/1660molecularly imprinted polymerssynthesis optimizationelectrochemistrywineantioxidantsfast method |
| spellingShingle | Marie Elhachem Elias Bou-Maroun Maher Abboud Philippe Cayot Richard G. Maroun Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine Foods molecularly imprinted polymers synthesis optimization electrochemistry wine antioxidants fast method |
| title | Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine |
| title_full | Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine |
| title_fullStr | Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine |
| title_full_unstemmed | Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine |
| title_short | Optimization of a Molecularly Imprinted Polymer Synthesis for a Rapid Detection of Caffeic Acid in Wine |
| title_sort | optimization of a molecularly imprinted polymer synthesis for a rapid detection of caffeic acid in wine |
| topic | molecularly imprinted polymers synthesis optimization electrochemistry wine antioxidants fast method |
| url | https://www.mdpi.com/2304-8158/12/8/1660 |
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