Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study
Background: Osteoporotic osteoarthritis (OP-OA) is a severe pathological form of OA, urgently requiring precise management strategies and more efficient interventions. Emodin (Emo), an effective ingredient found in the traditional Chinese medicine rhubarb, has been dEmonstrated to promote osteogenes...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Experimental Gerontology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S053155652400055X |
| _version_ | 1827274335618859008 |
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| author | Yibao Wei Junfeng Kang Zhenyuan Ma Taiyang Liao Peng Wu Peimin Wang Zhengquan Huang |
| author_facet | Yibao Wei Junfeng Kang Zhenyuan Ma Taiyang Liao Peng Wu Peimin Wang Zhengquan Huang |
| author_sort | Yibao Wei |
| collection | DOAJ |
| description | Background: Osteoporotic osteoarthritis (OP-OA) is a severe pathological form of OA, urgently requiring precise management strategies and more efficient interventions. Emodin (Emo), an effective ingredient found in the traditional Chinese medicine rhubarb, has been dEmonstrated to promote osteogenesis and inhibit extracellular matrix degradation. In this study, we aimed to investigate the interventional effects of Emo on the subchondral bone and cartilage of the knee joints in OP-OA model rats. Methods: Thirty-two SD rats were randomly and equally divided into sham, OP-OA, Emo low-dose, and Emo high-dose groups. Micro-CT scanning was conducted to examine the bone microstructure of the rat knee joints. H&E and Safranin O and Fast Green staining (SO&FG) were performed for the pathomorphological evaluation of the rat cartilage tissues. ELISA was used to estimate the rat serum expression levels of inflammatory factors, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Additionally, the CCK-8 assay was utilized for determining the viability of Emo-treated BMSCs. Western blot and real-time PCR analyses were also employed to measure the bone formation indexes and cartilage synthesis and decomposition indexes. Lastly, the osteogenic and chondrogenic differentiation efficiency of the BMSCs was investigated via Alizarin Red and Alcian Blue staining. Results: Emo intervention alleviated the bone microstructural disruption of the subchondral bone and articular cartilage in the OP-OA rats and up-regulated the expression of bone and cartilage anabolic metabolism indicators, decreased the expression of cartilage catabolism indicators, and diminished the expression of inflammatory factors in the rat serum (P<0.05). Furthermore, Emo reversed the decline in the osteogenic and chondrogenic differentiation ability of the BMSCs (P<0.05). Conclusion: Emo intervention mitigates bone loss and cartilage damage in OP-OA rats and promotes the osteogenic and chondrogenic differentiation of BMSCs. |
| first_indexed | 2024-04-24T12:51:48Z |
| format | Article |
| id | doaj.art-1751c712695f4b3d8515f115133a76da |
| institution | Directory Open Access Journal |
| issn | 1873-6815 |
| language | English |
| last_indexed | 2025-03-22T06:23:22Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental Gerontology |
| spelling | doaj.art-1751c712695f4b3d8515f115133a76da2024-04-26T04:58:31ZengElsevierExperimental Gerontology1873-68152024-06-01190112413Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical studyYibao Wei0Junfeng Kang1Zhenyuan Ma2Taiyang Liao3Peng Wu4Peimin Wang5Zhengquan Huang6Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, ChinaDepartment of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, China; Department of Orthopedics, Affiliated Hospital of Shanxi University of Chinese Medicine, ChinaDepartment of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, ChinaDepartment of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, ChinaDepartment of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, ChinaDepartment of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Corresponding authors at: Jiangsu Province Hospital of Chinese Medicine, China.Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, China; Jiangsu Province Hospital of Chinese Medicine, China; Corresponding authors at: Jiangsu Province Hospital of Chinese Medicine, China.Background: Osteoporotic osteoarthritis (OP-OA) is a severe pathological form of OA, urgently requiring precise management strategies and more efficient interventions. Emodin (Emo), an effective ingredient found in the traditional Chinese medicine rhubarb, has been dEmonstrated to promote osteogenesis and inhibit extracellular matrix degradation. In this study, we aimed to investigate the interventional effects of Emo on the subchondral bone and cartilage of the knee joints in OP-OA model rats. Methods: Thirty-two SD rats were randomly and equally divided into sham, OP-OA, Emo low-dose, and Emo high-dose groups. Micro-CT scanning was conducted to examine the bone microstructure of the rat knee joints. H&E and Safranin O and Fast Green staining (SO&FG) were performed for the pathomorphological evaluation of the rat cartilage tissues. ELISA was used to estimate the rat serum expression levels of inflammatory factors, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Additionally, the CCK-8 assay was utilized for determining the viability of Emo-treated BMSCs. Western blot and real-time PCR analyses were also employed to measure the bone formation indexes and cartilage synthesis and decomposition indexes. Lastly, the osteogenic and chondrogenic differentiation efficiency of the BMSCs was investigated via Alizarin Red and Alcian Blue staining. Results: Emo intervention alleviated the bone microstructural disruption of the subchondral bone and articular cartilage in the OP-OA rats and up-regulated the expression of bone and cartilage anabolic metabolism indicators, decreased the expression of cartilage catabolism indicators, and diminished the expression of inflammatory factors in the rat serum (P<0.05). Furthermore, Emo reversed the decline in the osteogenic and chondrogenic differentiation ability of the BMSCs (P<0.05). Conclusion: Emo intervention mitigates bone loss and cartilage damage in OP-OA rats and promotes the osteogenic and chondrogenic differentiation of BMSCs.http://www.sciencedirect.com/science/article/pii/S053155652400055XKnee osteoarthritisOsteoporosisSubchondral boneEmodinArticular cartilageOP-OA |
| spellingShingle | Yibao Wei Junfeng Kang Zhenyuan Ma Taiyang Liao Peng Wu Peimin Wang Zhengquan Huang Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study Experimental Gerontology Knee osteoarthritis Osteoporosis Subchondral bone Emodin Articular cartilage OP-OA |
| title | Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study |
| title_full | Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study |
| title_fullStr | Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study |
| title_full_unstemmed | Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study |
| title_short | Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study |
| title_sort | protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats a preclinical study |
| topic | Knee osteoarthritis Osteoporosis Subchondral bone Emodin Articular cartilage OP-OA |
| url | http://www.sciencedirect.com/science/article/pii/S053155652400055X |
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