Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs
Chicken coccidiosis is an inflammatory disease caused by the apicomplexan protozoal parasite Eimeria. Pro-inflammatory cytokines and chemokines activate hepatocyte receptors in the liver, altering the production and release of numerous proteins known as acute phase proteins (APPs), which eradicate...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2024-02-01
|
| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1516 |
| _version_ | 1797295717784485888 |
|---|---|
| author | Ghada M. Fathy Khalid M. Fararh Saher S. Abd El-hamied |
| author_facet | Ghada M. Fathy Khalid M. Fararh Saher S. Abd El-hamied |
| author_sort | Ghada M. Fathy |
| collection | DOAJ |
| description |
Chicken coccidiosis is an inflammatory disease caused by the apicomplexan protozoal parasite Eimeria. Pro-inflammatory cytokines and chemokines activate hepatocyte receptors in the liver, altering the production and release of numerous proteins known as acute phase proteins (APPs), which eradicate pathogenic germs, repair tissue damage, and promote health. These proteins have a significant impact on defense systems. The current investigation evaluated the levels of several acute phase proteins (haptoglobin (PIT54), ceruloplasmin, and fibrinogen) in the blood, their gene expression in the liver as well as the immunohistochemistry changes in the caecum and bursa of Fabricius of chickens infected with E. tenella before and after treatment with two different anticoccidial drugs (diclazuril and toltrazuril). The obtained results revealed that E. tenella infection in chickens produced considerable activation of numerous APPs, including haptoglobin, fibrinogen, and ceruloplasmin. Furthermore, the challenged birds’ caecum showed a high sensitivity to CD4, CD8, and SIgA. Anticoccidial medicines such as diclazuril or toltrazuril could considerably lower such APPs indicators and immunohistochemical alterations. In conclusion, haptoglobin, fibrinogen, and ceruloplasmin are considered as biomarkers for the APPs measurement in birds challenged with Eimeria. Moreover, diclazuril and toltrazuril are regarded as ideal anticoccidial medications to be used during chicken farming.
|
| first_indexed | 2024-03-07T21:52:01Z |
| format | Article |
| id | doaj.art-1752de973d934e13870287a123178bd5 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-07T21:52:01Z |
| publishDate | 2024-02-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-1752de973d934e13870287a123178bd52024-02-25T05:01:36ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-02-01142Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugsGhada M. Fathy0Khalid M. Fararh1Saher S. Abd El-hamied2Department of Clinical Pathology, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Zagazig, Egypt. Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Egypt. Department of Clinical Pathology, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Zagazig, Egypt. Chicken coccidiosis is an inflammatory disease caused by the apicomplexan protozoal parasite Eimeria. Pro-inflammatory cytokines and chemokines activate hepatocyte receptors in the liver, altering the production and release of numerous proteins known as acute phase proteins (APPs), which eradicate pathogenic germs, repair tissue damage, and promote health. These proteins have a significant impact on defense systems. The current investigation evaluated the levels of several acute phase proteins (haptoglobin (PIT54), ceruloplasmin, and fibrinogen) in the blood, their gene expression in the liver as well as the immunohistochemistry changes in the caecum and bursa of Fabricius of chickens infected with E. tenella before and after treatment with two different anticoccidial drugs (diclazuril and toltrazuril). The obtained results revealed that E. tenella infection in chickens produced considerable activation of numerous APPs, including haptoglobin, fibrinogen, and ceruloplasmin. Furthermore, the challenged birds’ caecum showed a high sensitivity to CD4, CD8, and SIgA. Anticoccidial medicines such as diclazuril or toltrazuril could considerably lower such APPs indicators and immunohistochemical alterations. In conclusion, haptoglobin, fibrinogen, and ceruloplasmin are considered as biomarkers for the APPs measurement in birds challenged with Eimeria. Moreover, diclazuril and toltrazuril are regarded as ideal anticoccidial medications to be used during chicken farming. https://advetresearch.com/index.php/AVR/article/view/1516ChickenEimeriaAcute phase proteins DiclazurilToltrazuril |
| spellingShingle | Ghada M. Fathy Khalid M. Fararh Saher S. Abd El-hamied Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs Journal of Advanced Veterinary Research Chicken Eimeria Acute phase proteins Diclazuril Toltrazuril |
| title | Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs |
| title_full | Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs |
| title_fullStr | Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs |
| title_full_unstemmed | Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs |
| title_short | Molecular and immune-histochemical changes in broiler chickens infected with Eimeria tenella and the protective effect of some anti-coccidial drugs |
| title_sort | molecular and immune histochemical changes in broiler chickens infected with eimeria tenella and the protective effect of some anti coccidial drugs |
| topic | Chicken Eimeria Acute phase proteins Diclazuril Toltrazuril |
| url | https://advetresearch.com/index.php/AVR/article/view/1516 |
| work_keys_str_mv | AT ghadamfathy molecularandimmunehistochemicalchangesinbroilerchickensinfectedwitheimeriatenellaandtheprotectiveeffectofsomeanticoccidialdrugs AT khalidmfararh molecularandimmunehistochemicalchangesinbroilerchickensinfectedwitheimeriatenellaandtheprotectiveeffectofsomeanticoccidialdrugs AT sahersabdelhamied molecularandimmunehistochemicalchangesinbroilerchickensinfectedwitheimeriatenellaandtheprotectiveeffectofsomeanticoccidialdrugs |