Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>)
Triterpene saponins exhibit various biological and pharmacological activities. However, the knowledge on saponin biosynthesis in tea plants (<i>Camellia sinensis</i> L.) is still limited. In this work, tea flower and seed samples at different developmental stages and leaves were collecte...
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MDPI AG
2022-02-01
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| Loạt: | Metabolites |
| Những chủ đề: | |
| Truy cập trực tuyến: | https://www.mdpi.com/2218-1989/12/3/204 |
| _version_ | 1827627807902007296 |
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| author | Cong Chen Huanqing Zhu Jiaxin Kang Hasitha Kalhari Warusawitharana Shuna Chen Kaixi Wang Fei Yu Yuanyuan Wu Puming He Youying Tu Bo Li |
| author_facet | Cong Chen Huanqing Zhu Jiaxin Kang Hasitha Kalhari Warusawitharana Shuna Chen Kaixi Wang Fei Yu Yuanyuan Wu Puming He Youying Tu Bo Li |
| author_sort | Cong Chen |
| collection | DOAJ |
| description | Triterpene saponins exhibit various biological and pharmacological activities. However, the knowledge on saponin biosynthesis in tea plants (<i>Camellia sinensis</i> L.) is still limited. In this work, tea flower and seed samples at different developmental stages and leaves were collected and analyzed with UPLC-PDA-MS and RNA sequencing for saponin determination and transcriptome comparison. The saponin content reached around 19% in the freshly mature seeds and 7% in the green flower buds, and decreased with the fruit ripeness and flower blooming. Almost no saponins were detected in leaf samples. PCA and KEGG analysis suggested that the gene expression pattern and secondary metabolism in TF1 and TS2 vs. leaf samples were significantly different. Weighted gene coexpression network analysis (WGCNA) uncovered two modules related to saponin content. The mevalonate (MVA) instead of 2-C-methyl-<span style="font-variant: small-caps;">d</span>-erythritol-4-phospate (MEP) pathway was responsible for saponin accumulation in tea plants, and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), diphosphomevalonate decarboxylase (MVD) and isopentenyl diphosphate isomerase (IDI) may be the key enzymes involved in saponin biosynthesis in tea seeds and flowers. Moreover, ten transcription factors (TFs) were predicted to regulate saponin biosynthesis in the tea plant. Taken together, our study provides a global insight into the saponin biosynthesis and accumulation in the tea plant. |
| first_indexed | 2024-03-09T13:21:04Z |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T13:21:04Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-1752eb101d584d7e918ec76780af6da22023-11-30T21:29:18ZengMDPI AGMetabolites2218-19892022-02-0112320410.3390/metabo12030204Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>)Cong Chen0Huanqing Zhu1Jiaxin Kang2Hasitha Kalhari Warusawitharana3Shuna Chen4Kaixi Wang5Fei Yu6Yuanyuan Wu7Puming He8Youying Tu9Bo Li10Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, ChinaTriterpene saponins exhibit various biological and pharmacological activities. However, the knowledge on saponin biosynthesis in tea plants (<i>Camellia sinensis</i> L.) is still limited. In this work, tea flower and seed samples at different developmental stages and leaves were collected and analyzed with UPLC-PDA-MS and RNA sequencing for saponin determination and transcriptome comparison. The saponin content reached around 19% in the freshly mature seeds and 7% in the green flower buds, and decreased with the fruit ripeness and flower blooming. Almost no saponins were detected in leaf samples. PCA and KEGG analysis suggested that the gene expression pattern and secondary metabolism in TF1 and TS2 vs. leaf samples were significantly different. Weighted gene coexpression network analysis (WGCNA) uncovered two modules related to saponin content. The mevalonate (MVA) instead of 2-C-methyl-<span style="font-variant: small-caps;">d</span>-erythritol-4-phospate (MEP) pathway was responsible for saponin accumulation in tea plants, and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), diphosphomevalonate decarboxylase (MVD) and isopentenyl diphosphate isomerase (IDI) may be the key enzymes involved in saponin biosynthesis in tea seeds and flowers. Moreover, ten transcription factors (TFs) were predicted to regulate saponin biosynthesis in the tea plant. Taken together, our study provides a global insight into the saponin biosynthesis and accumulation in the tea plant.https://www.mdpi.com/2218-1989/12/3/204saponinbiosynthesis<i>Camellia sinensis</i>seedsflowerstranscriptome |
| spellingShingle | Cong Chen Huanqing Zhu Jiaxin Kang Hasitha Kalhari Warusawitharana Shuna Chen Kaixi Wang Fei Yu Yuanyuan Wu Puming He Youying Tu Bo Li Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) Metabolites saponin biosynthesis <i>Camellia sinensis</i> seeds flowers transcriptome |
| title | Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) |
| title_full | Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) |
| title_fullStr | Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) |
| title_full_unstemmed | Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) |
| title_short | Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (<i>Camellia sinensis</i>) |
| title_sort | comparative transcriptome and phytochemical analysis provides insight into triterpene saponin biosynthesis in seeds and flowers of the tea plant i camellia sinensis i |
| topic | saponin biosynthesis <i>Camellia sinensis</i> seeds flowers transcriptome |
| url | https://www.mdpi.com/2218-1989/12/3/204 |
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