Studies on applying SNP markers to breeding drought-tolerant maize hybrids
The results of testing the International Maize and Wheat Improvement Center’s (CIMMYT’s) hybrid combinations, developed from hybrid 790 F2:3 lines and 10 parental lines, using two testers (CML451 and CLO2450) under optimal and managed drought conditions in Ninh Thuan, Vietnam, show that the average...
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2022-03-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/142 |
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| author | Nguyen Xuan Thang Bui Manh Cuong Dang Ngoc Ha Do Van Dung Sudha Nair M.T. Vinayan Gajanan Saykhedkar Raman Babu Doan Thi Bich Thao Tran Quang Dieu Nguyen Chi Thanh P.H. Zaidi |
| author_facet | Nguyen Xuan Thang Bui Manh Cuong Dang Ngoc Ha Do Van Dung Sudha Nair M.T. Vinayan Gajanan Saykhedkar Raman Babu Doan Thi Bich Thao Tran Quang Dieu Nguyen Chi Thanh P.H. Zaidi |
| author_sort | Nguyen Xuan Thang |
| collection | DOAJ |
| description |
The results of testing the International Maize and Wheat Improvement Center’s (CIMMYT’s) hybrid combinations, developed from hybrid 790 F2:3 lines and 10 parental lines, using two testers (CML451 and CLO2450) under optimal and managed drought conditions in Ninh Thuan, Vietnam, show that the average grain yield of the biparental (BP) groups of heterosis groups A and B is, respectively, 2.58-3.65 tons/ha and 2.56-2.76 tons/ha in drought conditions, and 4.24-5.02 tons/ha and 5.41-5.93 tons/ha in wellwatered conditions, respectively. By genotyping eight BP populations with 39,846 single nucleotide polymorphism (SNP) markers, CIMMYT experts identified 15 important gene regions that regulate grain yield associated with 15 SNP markers on chromosomes 3, 4, 5, 6, 7, 8, 9, and 10 which is useful for applying molecular markers in breeding drought-tolerant maize. On that basis, the Maize Research Institute of Vietnam studied and genotyped three populations, including 450 F2 families, with 96 SNPs using the Kompetitive Allele Specific PCR (KASP) genotyping method. The result was that 57 SNP markers related to drought tolerance were found useful to these populations. In addition, 27 F 2 families demonstrating drought tolerance and high grain yield were selected as primary materials for breeding maize hybrids tolerant to stresses and adaptive to climate change.
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| first_indexed | 2024-04-10T18:50:01Z |
| format | Article |
| id | doaj.art-1754ec91aa1c403f9dd7353198b504f8 |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:01Z |
| publishDate | 2022-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-1754ec91aa1c403f9dd7353198b504f82023-02-01T08:16:23ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-03-0162110.31276/VJSTE.62(1).55-61Studies on applying SNP markers to breeding drought-tolerant maize hybridsNguyen Xuan Thang0Bui Manh Cuong1Dang Ngoc Ha2Do Van Dung3Sudha Nair4M.T. Vinayan5Gajanan Saykhedkar6Raman Babu7Doan Thi Bich Thao8Tran Quang Dieu9Nguyen Chi Thanh10P.H. Zaidi11Maize Research Institute, VietnamMaize Research Institute, VietnamMaize Research Institute, VietnamMaize Research Institute, VietnamAsian Regional Maize Program, CIMMYT Int., IndiaAsian Regional Maize Program, CIMMYT Int., IndiaAsian Regional Maize Program, CIMMYT Int., IndiaPioneer Hi-Bred International Inc, USAMaize Research Institute, VietnamMaize Research Institute, VietnamMaize Research Institute, VietnamAsian Regional Maize Program, CIMMYT Int., India The results of testing the International Maize and Wheat Improvement Center’s (CIMMYT’s) hybrid combinations, developed from hybrid 790 F2:3 lines and 10 parental lines, using two testers (CML451 and CLO2450) under optimal and managed drought conditions in Ninh Thuan, Vietnam, show that the average grain yield of the biparental (BP) groups of heterosis groups A and B is, respectively, 2.58-3.65 tons/ha and 2.56-2.76 tons/ha in drought conditions, and 4.24-5.02 tons/ha and 5.41-5.93 tons/ha in wellwatered conditions, respectively. By genotyping eight BP populations with 39,846 single nucleotide polymorphism (SNP) markers, CIMMYT experts identified 15 important gene regions that regulate grain yield associated with 15 SNP markers on chromosomes 3, 4, 5, 6, 7, 8, 9, and 10 which is useful for applying molecular markers in breeding drought-tolerant maize. On that basis, the Maize Research Institute of Vietnam studied and genotyped three populations, including 450 F2 families, with 96 SNPs using the Kompetitive Allele Specific PCR (KASP) genotyping method. The result was that 57 SNP markers related to drought tolerance were found useful to these populations. In addition, 27 F 2 families demonstrating drought tolerance and high grain yield were selected as primary materials for breeding maize hybrids tolerant to stresses and adaptive to climate change. https://vietnamscience.vjst.vn/index.php/vjste/article/view/142droughtGWASKASPmaizeoptimal conditionsSNP markers |
| spellingShingle | Nguyen Xuan Thang Bui Manh Cuong Dang Ngoc Ha Do Van Dung Sudha Nair M.T. Vinayan Gajanan Saykhedkar Raman Babu Doan Thi Bich Thao Tran Quang Dieu Nguyen Chi Thanh P.H. Zaidi Studies on applying SNP markers to breeding drought-tolerant maize hybrids Vietnam Journal of Science, Technology and Engineering drought GWAS KASP maize optimal conditions SNP markers |
| title | Studies on applying SNP markers to breeding drought-tolerant maize hybrids |
| title_full | Studies on applying SNP markers to breeding drought-tolerant maize hybrids |
| title_fullStr | Studies on applying SNP markers to breeding drought-tolerant maize hybrids |
| title_full_unstemmed | Studies on applying SNP markers to breeding drought-tolerant maize hybrids |
| title_short | Studies on applying SNP markers to breeding drought-tolerant maize hybrids |
| title_sort | studies on applying snp markers to breeding drought tolerant maize hybrids |
| topic | drought GWAS KASP maize optimal conditions SNP markers |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/142 |
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