Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings
Proteome analysis of grafted cotton exposed to low-temperature stress can provide insights into the molecular mechanistic of chilling tolerance in plants. In this study, grafted and non-grafted cotton plants were exposed to chilling stress (10 °C/5 °C) for 7 d. After the stress, rootstock and scion...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/5/1152 |
| _version_ | 1797502160767811584 |
|---|---|
| author | Xin Zhang Yan Feng Aziz Khan Najeeb Ullah Zengqiang Li Saira Zaheer Ruiyang Zhou Zhiyong Zhang |
| author_facet | Xin Zhang Yan Feng Aziz Khan Najeeb Ullah Zengqiang Li Saira Zaheer Ruiyang Zhou Zhiyong Zhang |
| author_sort | Xin Zhang |
| collection | DOAJ |
| description | Proteome analysis of grafted cotton exposed to low-temperature stress can provide insights into the molecular mechanistic of chilling tolerance in plants. In this study, grafted and non-grafted cotton plants were exposed to chilling stress (10 °C/5 °C) for 7 d. After the stress, rootstock and scion samples were labeled by 8-plex iTRAQ (isobaric Tags for Relative and Absolute Quantification), followed by two-dimensional liquid chromatography separation and tandem mass spectrometry identification. In total, 68 differential proteins were identified that were induced by low-temperature stress and grafting, and these proteins regulate physiological functioning. Under low-temperature stress, in the cotton seedlings, the proteins responded to the MAPK signaling pathway and calcium signaling pathway enhanced, the metabolisms of carbohydrate, lipid, nucleotide, and amino acid had a tendency to intensify, the proteins related to protein folding and degradation were activated, along with the system of antioxidant enzymes to offset cellular oxidative damage. In contrast, chilling stress reduced oxidative phosphorylation, photosynthesis, and carbon fixation. These data indicated that the physiological changes in cotton seedlings comprise a complex biological process, and the ability of plants to resist this stress can be improved after grafting onto a vigorous rootstock, although this was not obvious in the young plants. Further studies of low-temperature stress and/or graft-related differences in proteins could lead to the identification of new genes associated with chilling tolerance in plants. These data provide the basis for further studies on the molecular mechanism of chilling tolerance and the relationship of grafting and chilling tolerance in cotton. |
| first_indexed | 2024-03-10T03:29:58Z |
| format | Article |
| id | doaj.art-1755d65522724f778f7ab7de1e8cfe6f |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:29:58Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-1755d65522724f778f7ab7de1e8cfe6f2023-11-23T09:43:35ZengMDPI AGAgronomy2073-43952022-05-01125115210.3390/agronomy12051152Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton SeedlingsXin Zhang0Yan Feng1Aziz Khan2Najeeb Ullah3Zengqiang Li4Saira Zaheer5Ruiyang Zhou6Zhiyong Zhang7Henan Collaborative Innovation Centre of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, ChinaHenan Collaborative Innovation Centre of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Agriculture, Guangxi University, Nanning 530005, ChinaFaculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, Seri Begawan BE1410, BruneiHenan Collaborative Innovation Centre of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, ChinaDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanCollege of Agriculture, Guangxi University, Nanning 530005, ChinaHenan Collaborative Innovation Centre of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, ChinaProteome analysis of grafted cotton exposed to low-temperature stress can provide insights into the molecular mechanistic of chilling tolerance in plants. In this study, grafted and non-grafted cotton plants were exposed to chilling stress (10 °C/5 °C) for 7 d. After the stress, rootstock and scion samples were labeled by 8-plex iTRAQ (isobaric Tags for Relative and Absolute Quantification), followed by two-dimensional liquid chromatography separation and tandem mass spectrometry identification. In total, 68 differential proteins were identified that were induced by low-temperature stress and grafting, and these proteins regulate physiological functioning. Under low-temperature stress, in the cotton seedlings, the proteins responded to the MAPK signaling pathway and calcium signaling pathway enhanced, the metabolisms of carbohydrate, lipid, nucleotide, and amino acid had a tendency to intensify, the proteins related to protein folding and degradation were activated, along with the system of antioxidant enzymes to offset cellular oxidative damage. In contrast, chilling stress reduced oxidative phosphorylation, photosynthesis, and carbon fixation. These data indicated that the physiological changes in cotton seedlings comprise a complex biological process, and the ability of plants to resist this stress can be improved after grafting onto a vigorous rootstock, although this was not obvious in the young plants. Further studies of low-temperature stress and/or graft-related differences in proteins could lead to the identification of new genes associated with chilling tolerance in plants. These data provide the basis for further studies on the molecular mechanism of chilling tolerance and the relationship of grafting and chilling tolerance in cotton.https://www.mdpi.com/2073-4395/12/5/1152cottongraftingproteomechillingiTRAQ |
| spellingShingle | Xin Zhang Yan Feng Aziz Khan Najeeb Ullah Zengqiang Li Saira Zaheer Ruiyang Zhou Zhiyong Zhang Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings Agronomy cotton grafting proteome chilling iTRAQ |
| title | Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings |
| title_full | Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings |
| title_fullStr | Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings |
| title_full_unstemmed | Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings |
| title_short | Quantitative Proteomics-Based Analysis Reveals Molecular Mechanisms of Chilling Tolerance in Grafted Cotton Seedlings |
| title_sort | quantitative proteomics based analysis reveals molecular mechanisms of chilling tolerance in grafted cotton seedlings |
| topic | cotton grafting proteome chilling iTRAQ |
| url | https://www.mdpi.com/2073-4395/12/5/1152 |
| work_keys_str_mv | AT xinzhang quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT yanfeng quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT azizkhan quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT najeebullah quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT zengqiangli quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT sairazaheer quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT ruiyangzhou quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings AT zhiyongzhang quantitativeproteomicsbasedanalysisrevealsmolecularmechanismsofchillingtoleranceingraftedcottonseedlings |