Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia
Abstract The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic later...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2018-05-01
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| Series: | Translational Psychiatry |
| Online Access: | https://doi.org/10.1038/s41398-018-0107-9 |
| _version_ | 1818566283563630592 |
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| author | Loes M. Olde Loohuis Serghei Mangul Anil P. S. Ori Guillaume Jospin David Koslicki Harry Taegyun Yang Timothy Wu Marco P. Boks Catherine Lomen-Hoerth Martina Wiedau-Pazos Rita M. Cantor Willem M. de Vos René S. Kahn Eleazar Eskin Roel A. Ophoff |
| author_facet | Loes M. Olde Loohuis Serghei Mangul Anil P. S. Ori Guillaume Jospin David Koslicki Harry Taegyun Yang Timothy Wu Marco P. Boks Catherine Lomen-Hoerth Martina Wiedau-Pazos Rita M. Cantor Willem M. de Vos René S. Kahn Eleazar Eskin Roel A. Ophoff |
| author_sort | Loes M. Olde Loohuis |
| collection | DOAJ |
| description | Abstract The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case–control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8+ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia. |
| first_indexed | 2024-12-14T01:51:43Z |
| format | Article |
| id | doaj.art-17562bdf44234e028412afcbfe62fa09 |
| institution | Directory Open Access Journal |
| issn | 2158-3188 |
| language | English |
| last_indexed | 2024-12-14T01:51:43Z |
| publishDate | 2018-05-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Translational Psychiatry |
| spelling | doaj.art-17562bdf44234e028412afcbfe62fa092022-12-21T23:21:21ZengNature Publishing GroupTranslational Psychiatry2158-31882018-05-01811910.1038/s41398-018-0107-9Transcriptome analysis in whole blood reveals increased microbial diversity in schizophreniaLoes M. Olde Loohuis0Serghei Mangul1Anil P. S. Ori2Guillaume Jospin3David Koslicki4Harry Taegyun Yang5Timothy Wu6Marco P. Boks7Catherine Lomen-Hoerth8Martina Wiedau-Pazos9Rita M. Cantor10Willem M. de Vos11René S. Kahn12Eleazar Eskin13Roel A. Ophoff14Center for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, University California Los AngelesDepartment of Computer, Science University of California Los AngelesCenter for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, University California Los AngelesDavis Genome Center, University of CaliforniaMathematics Department, Oregon State UniversityDepartment of Computer, Science University of California Los AngelesCenter for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, University California Los AngelesDepartment of Psychiatry, Brain Center Rudolf Magnus, University Medical Center UtrechtDepartment of Neurology, University of California San FranciscoDepartment of Neurology, David Geffen School of Medicine, University of California Los AngelesDepartment of Human Genetics, University of California Los AngelesLaboratory of Microbiology, Wageningen UniversityDepartment of Psychiatry, Brain Center Rudolf Magnus, University Medical Center UtrechtDepartment of Computer, Science University of California Los AngelesCenter for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, University California Los AngelesAbstract The role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case–control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8+ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia.https://doi.org/10.1038/s41398-018-0107-9 |
| spellingShingle | Loes M. Olde Loohuis Serghei Mangul Anil P. S. Ori Guillaume Jospin David Koslicki Harry Taegyun Yang Timothy Wu Marco P. Boks Catherine Lomen-Hoerth Martina Wiedau-Pazos Rita M. Cantor Willem M. de Vos René S. Kahn Eleazar Eskin Roel A. Ophoff Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia Translational Psychiatry |
| title | Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| title_full | Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| title_fullStr | Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| title_full_unstemmed | Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| title_short | Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| title_sort | transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia |
| url | https://doi.org/10.1038/s41398-018-0107-9 |
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