Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway
Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461600089X |
| _version_ | 1818723364874747904 |
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| author | Zhenzhen Ge Wei Zhu Jinming Peng Xiangyi Deng Chunmei Li |
| author_facet | Zhenzhen Ge Wei Zhu Jinming Peng Xiangyi Deng Chunmei Li |
| author_sort | Zhenzhen Ge |
| collection | DOAJ |
| description | Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon tannin on the expression of genes critical for cholesterol absorption and cholesterol efflux. Our results indicated that persimmon tannin reduced the uptake of 22-NBD-cholesterol and inhibited the accumulation of cholesterol significantly in HepG2 and Caco-2 cell lines. The mRNA and proteins were quantified using real time PCR and western blot, respectively. The expression of SREBP-2 gene, critical for cholesterol synthesis, and NPC1L1 involved in cholesterol absorption were significantly inhibited by persimmon tannin. Moreover, persimmon tannin showed potential in promoting cholesterol efflux through up-regulating mRNA and/or protein levels of ABCA1, ABCG1, SR-BI, CYP7A1 and ABCG5/G8. However, the regulation effects of persimmon tannin on cholesterol homeostasis in both cell lines were LXRα independent. |
| first_indexed | 2024-12-17T21:09:21Z |
| format | Article |
| id | doaj.art-17595febee6048cb9a9f70e79cef3498 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:09:21Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-17595febee6048cb9a9f70e79cef34982022-12-21T21:32:29ZengElsevierJournal of Functional Foods1756-46462016-05-0123283293Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathwayZhenzhen Ge0Wei Zhu1Jinming Peng2Xiangyi Deng3Chunmei Li4College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Ministry of Education, Key Laboratory of Environment Correlative Food Science, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Tel.: 86 27 87282966; fax: 86 27 87282966.Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon tannin on the expression of genes critical for cholesterol absorption and cholesterol efflux. Our results indicated that persimmon tannin reduced the uptake of 22-NBD-cholesterol and inhibited the accumulation of cholesterol significantly in HepG2 and Caco-2 cell lines. The mRNA and proteins were quantified using real time PCR and western blot, respectively. The expression of SREBP-2 gene, critical for cholesterol synthesis, and NPC1L1 involved in cholesterol absorption were significantly inhibited by persimmon tannin. Moreover, persimmon tannin showed potential in promoting cholesterol efflux through up-regulating mRNA and/or protein levels of ABCA1, ABCG1, SR-BI, CYP7A1 and ABCG5/G8. However, the regulation effects of persimmon tannin on cholesterol homeostasis in both cell lines were LXRα independent.http://www.sciencedirect.com/science/article/pii/S175646461600089XPersimmon tanninCholesterol homeostasisLXRαABCA1CYP7A1 |
| spellingShingle | Zhenzhen Ge Wei Zhu Jinming Peng Xiangyi Deng Chunmei Li Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway Journal of Functional Foods Persimmon tannin Cholesterol homeostasis LXRα ABCA1 CYP7A1 |
| title | Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway |
| title_full | Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway |
| title_fullStr | Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway |
| title_full_unstemmed | Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway |
| title_short | Persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by LXRα independent pathway |
| title_sort | persimmon tannin regulates the expression of genes critical for cholesterol absorption and cholesterol efflux by lxrα independent pathway |
| topic | Persimmon tannin Cholesterol homeostasis LXRα ABCA1 CYP7A1 |
| url | http://www.sciencedirect.com/science/article/pii/S175646461600089X |
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