<i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits
Soil-borne plant diseases seriously threaten the tomato industry worldwide. Currently, eco-friendly biocontrol strategies have been increasingly considered as effective approaches to control the incidence of disease. In this study, we identified bacteria that could be used as biocontrol agents to mi...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8527 |
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| author | Honghong Dong Ruixiang Gao Yijie Dong Qing Yao Honghui Zhu |
| author_facet | Honghong Dong Ruixiang Gao Yijie Dong Qing Yao Honghui Zhu |
| author_sort | Honghong Dong |
| collection | DOAJ |
| description | Soil-borne plant diseases seriously threaten the tomato industry worldwide. Currently, eco-friendly biocontrol strategies have been increasingly considered as effective approaches to control the incidence of disease. In this study, we identified bacteria that could be used as biocontrol agents to mitigate the growth and spread of the pathogens causing economically significant diseases of tomato plants, such as tomato bacterial wilt and tomato Fusarium wilt. Specifically, we isolated a strain of <i>Bacillus velezensis</i> (RC116) from tomato rhizosphere soil in Guangdong Province, China, with high biocontrol potential and confirmed its identity using both morphological and molecular approaches. RC116 not only produced protease, amylase, lipase, and siderophores but also secreted indoleacetic acid, and dissolved organophosphorus in vivo. Moreover, 12 <i>Bacillus</i> biocontrol maker genes associated with antibiotics biosynthesis could be amplified in the RC116 genome. Extracellular secreted proteins of RC116 also exhibited strong lytic activity against <i>Ralstonia solanacearum</i> and <i>Fusarium oxysporum</i> f. sp. <i>Lycopersici</i>. Pot experiments showed that the biocontrol efficacy of RC116 against tomato bacteria wilt was 81%, and consequently, RC116 significantly promoted the growth of tomato plantlets. Based on these multiple biocontrol traits, RC116 is expected to be developed into a broad-spectrum biocontrol agent. Although several previous studies have examined the utility of <i>B. velezensis</i> for the control of fungal diseases, few studies to date have evaluated the utility of <i>B. velezensis</i> for the control of bacterial diseases. Our study fills this research gap. Collectively, our findings provide new insights that will aid the control of soil-borne diseases, as well as future studies of <i>B. velezensis</i> strains. |
| first_indexed | 2024-03-11T03:40:14Z |
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| language | English |
| last_indexed | 2024-03-11T03:40:14Z |
| publishDate | 2023-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-175d7c82d7854c4d86234e7e8191ee112023-11-18T01:37:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410852710.3390/ijms24108527<i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol TraitsHonghong Dong0Ruixiang Gao1Yijie Dong2Qing Yao3Honghui Zhu4Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaSoil-borne plant diseases seriously threaten the tomato industry worldwide. Currently, eco-friendly biocontrol strategies have been increasingly considered as effective approaches to control the incidence of disease. In this study, we identified bacteria that could be used as biocontrol agents to mitigate the growth and spread of the pathogens causing economically significant diseases of tomato plants, such as tomato bacterial wilt and tomato Fusarium wilt. Specifically, we isolated a strain of <i>Bacillus velezensis</i> (RC116) from tomato rhizosphere soil in Guangdong Province, China, with high biocontrol potential and confirmed its identity using both morphological and molecular approaches. RC116 not only produced protease, amylase, lipase, and siderophores but also secreted indoleacetic acid, and dissolved organophosphorus in vivo. Moreover, 12 <i>Bacillus</i> biocontrol maker genes associated with antibiotics biosynthesis could be amplified in the RC116 genome. Extracellular secreted proteins of RC116 also exhibited strong lytic activity against <i>Ralstonia solanacearum</i> and <i>Fusarium oxysporum</i> f. sp. <i>Lycopersici</i>. Pot experiments showed that the biocontrol efficacy of RC116 against tomato bacteria wilt was 81%, and consequently, RC116 significantly promoted the growth of tomato plantlets. Based on these multiple biocontrol traits, RC116 is expected to be developed into a broad-spectrum biocontrol agent. Although several previous studies have examined the utility of <i>B. velezensis</i> for the control of fungal diseases, few studies to date have evaluated the utility of <i>B. velezensis</i> for the control of bacterial diseases. Our study fills this research gap. Collectively, our findings provide new insights that will aid the control of soil-borne diseases, as well as future studies of <i>B. velezensis</i> strains.https://www.mdpi.com/1422-0067/24/10/8527<i>Rhizosphere microorganisms</i>soil-borne diseaseantagonism activitybiocontrol genesextracellular lyase |
| spellingShingle | Honghong Dong Ruixiang Gao Yijie Dong Qing Yao Honghui Zhu <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits International Journal of Molecular Sciences <i>Rhizosphere microorganisms</i> soil-borne disease antagonism activity biocontrol genes extracellular lyase |
| title | <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits |
| title_full | <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits |
| title_fullStr | <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits |
| title_full_unstemmed | <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits |
| title_short | <i>Bacillus velezensis</i> RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits |
| title_sort | i bacillus velezensis i rc116 inhibits the pathogens of bacterial wilt and fusarium wilt in tomato with multiple biocontrol traits |
| topic | <i>Rhizosphere microorganisms</i> soil-borne disease antagonism activity biocontrol genes extracellular lyase |
| url | https://www.mdpi.com/1422-0067/24/10/8527 |
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