Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i>
Shiga toxin-producing <i>Escherichia coli</i> (STEC) is one of the leading causes of foodborne illnesses in North America and can lead to severe symptoms, with increased fatality risk for young children. While <i>E. coli</i> O157:H7 remains the dominant STEC serotype associat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2356 |
| _version_ | 1797466299001995264 |
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| author | Janet T. Lin Sarah Kirst Stevan Cucić Alexandra Klem Yi-Min She Andrew M. Kropinski Hany Anany |
| author_facet | Janet T. Lin Sarah Kirst Stevan Cucić Alexandra Klem Yi-Min She Andrew M. Kropinski Hany Anany |
| author_sort | Janet T. Lin |
| collection | DOAJ |
| description | Shiga toxin-producing <i>Escherichia coli</i> (STEC) is one of the leading causes of foodborne illnesses in North America and can lead to severe symptoms, with increased fatality risk for young children. While <i>E. coli</i> O157:H7 remains the dominant STEC serotype associated with foodborne outbreaks, there has been an increasing number of non-O157 STEC outbreaks in recent years. For the food industry, lytic bacteriophages offer an organic, self-limiting alternative to pathogen reduction—one that could replace or reduce the use of chemical and physical food processing methods. From EHEC-enriched sewage, we isolated a novel bacteriophage, vB_EcoM-4HA13 (4HA13). Phenotypic characterizations revealed 4HA13 to possess a myoviral morphotype, with a high specificity to non-motile O111 serotype, and a long latent period (90 min). Through genomic analyses, this 52,401-bp dsDNA phage was found to contain 81 CDS, but no detectable presence of antibiotic resistance, integrase, or virulence genes. A BLASTn search for each of the identified 81 CDS yielded homologues with low levels of similarity. Comparison of RNA polymerase and terminase large subunit amino acid sequences led to the proposal and acceptance of a new bacteriophage family, <i>Chaseviridae</i>, with 4HA13 representing a new species and genus. The discovery of this phage has broadened our current knowledge of bacteriophage diversity. |
| first_indexed | 2024-03-09T18:33:53Z |
| format | Article |
| id | doaj.art-175f7f01514843b285149ee85a4465e2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T18:33:53Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-175f7f01514843b285149ee85a4465e22023-11-24T07:15:54ZengMDPI AGViruses1999-49152022-10-011411235610.3390/v14112356Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i>Janet T. Lin0Sarah Kirst1Stevan Cucić2Alexandra Klem3Yi-Min She4Andrew M. Kropinski5Hany Anany6Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaDepartment of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaNorthern Ontario School of Medicine, Laurentian University, Sudbury, ON P3E 2C6, CanadaCentre for Oncology, Radiopharmaceuticals and Research, Biologic and Radiopharmaceutical Drugs Directorate, Health Canada, Ottawa, ON K1A 0K9, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaShiga toxin-producing <i>Escherichia coli</i> (STEC) is one of the leading causes of foodborne illnesses in North America and can lead to severe symptoms, with increased fatality risk for young children. While <i>E. coli</i> O157:H7 remains the dominant STEC serotype associated with foodborne outbreaks, there has been an increasing number of non-O157 STEC outbreaks in recent years. For the food industry, lytic bacteriophages offer an organic, self-limiting alternative to pathogen reduction—one that could replace or reduce the use of chemical and physical food processing methods. From EHEC-enriched sewage, we isolated a novel bacteriophage, vB_EcoM-4HA13 (4HA13). Phenotypic characterizations revealed 4HA13 to possess a myoviral morphotype, with a high specificity to non-motile O111 serotype, and a long latent period (90 min). Through genomic analyses, this 52,401-bp dsDNA phage was found to contain 81 CDS, but no detectable presence of antibiotic resistance, integrase, or virulence genes. A BLASTn search for each of the identified 81 CDS yielded homologues with low levels of similarity. Comparison of RNA polymerase and terminase large subunit amino acid sequences led to the proposal and acceptance of a new bacteriophage family, <i>Chaseviridae</i>, with 4HA13 representing a new species and genus. The discovery of this phage has broadened our current knowledge of bacteriophage diversity.https://www.mdpi.com/1999-4915/14/11/2356bacteriophage<i>Escherichia coli</i><i>Chaseviridae</i>STEC O111-specific bacteriophagegenomicsproteomics |
| spellingShingle | Janet T. Lin Sarah Kirst Stevan Cucić Alexandra Klem Yi-Min She Andrew M. Kropinski Hany Anany Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> Viruses bacteriophage <i>Escherichia coli</i> <i>Chaseviridae</i> STEC O111-specific bacteriophage genomics proteomics |
| title | Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> |
| title_full | Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> |
| title_fullStr | Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> |
| title_full_unstemmed | Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> |
| title_short | Isolation, Characterization, and Genome Analysis of a Novel Bacteriophage, <i>Escherichia</i> Phage vB_EcoM-4HA13, Representing a New Phage Genus in the Novel Phage Family <i>Chaseviridae</i> |
| title_sort | isolation characterization and genome analysis of a novel bacteriophage i escherichia i phage vb ecom 4ha13 representing a new phage genus in the novel phage family i chaseviridae i |
| topic | bacteriophage <i>Escherichia coli</i> <i>Chaseviridae</i> STEC O111-specific bacteriophage genomics proteomics |
| url | https://www.mdpi.com/1999-4915/14/11/2356 |
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