Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns
Abstract Because cotton is the most important fiber crop, the differentiation and development mechanism of cotton fiber cells has been a hot topic in cotton research. NAC (for NAM, ATAF, and CUC2) is a class of plant‐specific transcription factor families that regulate multiple aspects of plant grow...
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Food and Energy Security |
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| Online Access: | https://doi.org/10.1002/fes3.407 |
| _version_ | 1798041378094055424 |
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| author | Guifeng Wang Xiaomin Yue Zongqin Feng Lijuan Cai Na Li Fang Geng Chuanjie Xu Lichen Wang Depeng Wang Shah Fahad |
| author_facet | Guifeng Wang Xiaomin Yue Zongqin Feng Lijuan Cai Na Li Fang Geng Chuanjie Xu Lichen Wang Depeng Wang Shah Fahad |
| author_sort | Guifeng Wang |
| collection | DOAJ |
| description | Abstract Because cotton is the most important fiber crop, the differentiation and development mechanism of cotton fiber cells has been a hot topic in cotton research. NAC (for NAM, ATAF, and CUC2) is a class of plant‐specific transcription factor families that regulate multiple aspects of plant growth and development. Previously, we identified 33 differentially expressed NAC genes in upland cotton based on fiber‐related transcriptome profiling. In this study, we conducted chromosome localization, systematic evolution, and gene structural analysis of 242 NAC genes genome‐wide based on a high‐quality reference genome of upland cotton. In addition, the expression pattern analysis of 16 differentially expressed GhNACs was carried out by RT–PCR using ovules at early fiber initiation stages of n2 recessive naked seed mutant (n2NSM) and Xuzhou142 fuzzless–lintless mutant (Xu142fl). The results showed that GhNAC230 and GhNAC115 were most closely related to AtSND1 and downregulated in ovules of Xu142fl compared with n2NSM. Quantitative RT–PCR (qRT–PCR) analysis of GhNAC230 demonstrated its gradual accumulation from −1 days postanthesis (DPA) to 1 DPA in n2NSM and further confirmed its downregulated expression in 1 DPA ovules of Xu142fl compared to n2NSM. The current study predicted a pair of NAC genes that might be key candidate genes that are crucial for lint fiber development. |
| first_indexed | 2024-04-11T22:20:42Z |
| format | Article |
| id | doaj.art-1762c68eb46d41388c4e5127f79316ea |
| institution | Directory Open Access Journal |
| issn | 2048-3694 |
| language | English |
| last_indexed | 2024-04-11T22:20:42Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food and Energy Security |
| spelling | doaj.art-1762c68eb46d41388c4e5127f79316ea2022-12-22T04:00:07ZengWileyFood and Energy Security2048-36942022-08-01113n/an/a10.1002/fes3.407Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patternsGuifeng Wang0Xiaomin Yue1Zongqin Feng2Lijuan Cai3Na Li4Fang Geng5Chuanjie Xu6Lichen Wang7Depeng Wang8Shah Fahad9School of Resources and Environmental Engineering Wuhan University of Technology Wuhan Hubei ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaCollege of Life Science Linyi University Linyi Shandong ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops Hainan University Haikou ChinaAbstract Because cotton is the most important fiber crop, the differentiation and development mechanism of cotton fiber cells has been a hot topic in cotton research. NAC (for NAM, ATAF, and CUC2) is a class of plant‐specific transcription factor families that regulate multiple aspects of plant growth and development. Previously, we identified 33 differentially expressed NAC genes in upland cotton based on fiber‐related transcriptome profiling. In this study, we conducted chromosome localization, systematic evolution, and gene structural analysis of 242 NAC genes genome‐wide based on a high‐quality reference genome of upland cotton. In addition, the expression pattern analysis of 16 differentially expressed GhNACs was carried out by RT–PCR using ovules at early fiber initiation stages of n2 recessive naked seed mutant (n2NSM) and Xuzhou142 fuzzless–lintless mutant (Xu142fl). The results showed that GhNAC230 and GhNAC115 were most closely related to AtSND1 and downregulated in ovules of Xu142fl compared with n2NSM. Quantitative RT–PCR (qRT–PCR) analysis of GhNAC230 demonstrated its gradual accumulation from −1 days postanthesis (DPA) to 1 DPA in n2NSM and further confirmed its downregulated expression in 1 DPA ovules of Xu142fl compared to n2NSM. The current study predicted a pair of NAC genes that might be key candidate genes that are crucial for lint fiber development.https://doi.org/10.1002/fes3.407fiber initiationn2 recessive naked seed mutant (n2NSM)NAC transcription factorsupland cottonXuzhou142 fuzzless–lintless mutant (Xu142fl) |
| spellingShingle | Guifeng Wang Xiaomin Yue Zongqin Feng Lijuan Cai Na Li Fang Geng Chuanjie Xu Lichen Wang Depeng Wang Shah Fahad Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns Food and Energy Security fiber initiation n2 recessive naked seed mutant (n2NSM) NAC transcription factors upland cotton Xuzhou142 fuzzless–lintless mutant (Xu142fl) |
| title | Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns |
| title_full | Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns |
| title_fullStr | Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns |
| title_full_unstemmed | Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns |
| title_short | Identification of AtSND1 homologous NAC genes related to cotton fiber development, in silico analyses, and gene expression patterns |
| title_sort | identification of atsnd1 homologous nac genes related to cotton fiber development in silico analyses and gene expression patterns |
| topic | fiber initiation n2 recessive naked seed mutant (n2NSM) NAC transcription factors upland cotton Xuzhou142 fuzzless–lintless mutant (Xu142fl) |
| url | https://doi.org/10.1002/fes3.407 |
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