Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1)
Follicular neoplasms are classified as benign or malignant depending on the presence or absence of capsular and/or vascular invasion. Due to incomplete capsular penetration or equivocal vascular invasion, the evaluation of these features can be challenging using histologic examination. In the curren...
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MDPI AG
2023-06-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/13/3404 |
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| author | Yuan Rong Cesar Torres-Luna George Tuszynski Richard Siderits Frank N. Chang |
| author_facet | Yuan Rong Cesar Torres-Luna George Tuszynski Richard Siderits Frank N. Chang |
| author_sort | Yuan Rong |
| collection | DOAJ |
| description | Follicular neoplasms are classified as benign or malignant depending on the presence or absence of capsular and/or vascular invasion. Due to incomplete capsular penetration or equivocal vascular invasion, the evaluation of these features can be challenging using histologic examination. In the current study, we analyzed the involvement of G-protein coupled receptor-associated sorting protein 1 (GASP-1) in the development and progression of thyroid neoplasms. Affinity-purified anti-GASP-1 polyclonal antibodies were used for routine immunohistochemistry (IHC) analysis. Thyroid tissue microarrays containing normal thyroid tissue, follicular adenoma, follicular carcinoma, papillary thyroid carcinoma, and anaplastic carcinoma were analyzed. We found that the level of GASP-1 expression can differentiate follicular adenoma from follicular carcinoma. When numerous cases were scored for GASP-1 expression by a board-certified pathologist, we found that GASP-1 expression is 7-fold higher in thyroid malignant neoplasms compared to normal thyroid tissue, and about 4-fold higher in follicular carcinoma compared to follicular adenoma. In follicular adenoma tissues, we observed the presence of many mini-glands that are enriched in GASP-1 and some mini-glands contain as few as three cells. GASP-1 IHC also possesses several advantages over the conventional H&E and can be used to identify early thyroid cancer and monitor cancer progression. |
| first_indexed | 2024-03-11T01:44:50Z |
| format | Article |
| id | doaj.art-17639799492e4cff89055fe5e4d561bd |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T01:44:50Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-17639799492e4cff89055fe5e4d561bd2023-11-18T16:16:31ZengMDPI AGCancers2072-66942023-06-011513340410.3390/cancers15133404Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1)Yuan Rong0Cesar Torres-Luna1George Tuszynski2Richard Siderits3Frank N. Chang4Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, PA 19140, USAHalcyon Diagnostics, 1200 Corporate Blvd. Ste. 10C, Lancaster, PA 17601, USAHalcyon Diagnostics, 1200 Corporate Blvd. Ste. 10C, Lancaster, PA 17601, USADepartment of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USAHalcyon Diagnostics, 1200 Corporate Blvd. Ste. 10C, Lancaster, PA 17601, USAFollicular neoplasms are classified as benign or malignant depending on the presence or absence of capsular and/or vascular invasion. Due to incomplete capsular penetration or equivocal vascular invasion, the evaluation of these features can be challenging using histologic examination. In the current study, we analyzed the involvement of G-protein coupled receptor-associated sorting protein 1 (GASP-1) in the development and progression of thyroid neoplasms. Affinity-purified anti-GASP-1 polyclonal antibodies were used for routine immunohistochemistry (IHC) analysis. Thyroid tissue microarrays containing normal thyroid tissue, follicular adenoma, follicular carcinoma, papillary thyroid carcinoma, and anaplastic carcinoma were analyzed. We found that the level of GASP-1 expression can differentiate follicular adenoma from follicular carcinoma. When numerous cases were scored for GASP-1 expression by a board-certified pathologist, we found that GASP-1 expression is 7-fold higher in thyroid malignant neoplasms compared to normal thyroid tissue, and about 4-fold higher in follicular carcinoma compared to follicular adenoma. In follicular adenoma tissues, we observed the presence of many mini-glands that are enriched in GASP-1 and some mini-glands contain as few as three cells. GASP-1 IHC also possesses several advantages over the conventional H&E and can be used to identify early thyroid cancer and monitor cancer progression.https://www.mdpi.com/2072-6694/15/13/3404thyroid cancer biomarkerthyroid noduleimmunohistochemistryGASP-1cancer prevention |
| spellingShingle | Yuan Rong Cesar Torres-Luna George Tuszynski Richard Siderits Frank N. Chang Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) Cancers thyroid cancer biomarker thyroid nodule immunohistochemistry GASP-1 cancer prevention |
| title | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) |
| title_full | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) |
| title_fullStr | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) |
| title_full_unstemmed | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) |
| title_short | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) |
| title_sort | differentiating thyroid follicular adenoma from follicular carcinoma via g protein coupled receptor associated sorting protein 1 gasp 1 |
| topic | thyroid cancer biomarker thyroid nodule immunohistochemistry GASP-1 cancer prevention |
| url | https://www.mdpi.com/2072-6694/15/13/3404 |
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