Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings
Phospholipase C is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phosphoinositide-specific PLC (PI-PLC) and non-specific PLC (NPC), depending on its hydrolytic substrate. In maize, the function of phospholipase C has not been well characterized. In this stu...
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MDPI AG
2022-06-01
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| Series: | Genes |
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| author | Yulei Wei Xinyu Liu Shengnan Ge Haiyang Zhang Xinyang Che Shiyuan Liu Debin Liu Huixin Li Xinru Gu Lin He Zuotong Li Jingyu Xu |
| author_facet | Yulei Wei Xinyu Liu Shengnan Ge Haiyang Zhang Xinyang Che Shiyuan Liu Debin Liu Huixin Li Xinru Gu Lin He Zuotong Li Jingyu Xu |
| author_sort | Yulei Wei |
| collection | DOAJ |
| description | Phospholipase C is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phosphoinositide-specific PLC (PI-PLC) and non-specific PLC (NPC), depending on its hydrolytic substrate. In maize, the function of phospholipase C has not been well characterized. In this study, the phospholipase C inhibitor neomycin sulfate (NS, 100 mM) was applied to maize seedlings to investigate the function of maize PLC. Under the treatment of neomycin sulfate, the growth and development of maize seedlings were impaired, and the leaves were gradually etiolated and wilted. The analysis of physiological and biochemical parameters revealed that inhibition of phospholipase C affected photosynthesis, photosynthetic pigment accumulation, carbon metabolism and the stability of the cell membrane. High-throughput RNA-seq was conducted, and differentially expressed genes (DEGS) were found significantly enriched in photosynthesis and carbon metabolism pathways. When phospholipase C activity was inhibited, the expression of genes related to photosynthetic pigment accumulation was decreased, which led to lowered chlorophyll. Most of the genes related to PSI, PSII and TCA cycles were down-regulated and the net photosynthesis was decreased. Meanwhile, genes related to starch and sucrose metabolism, the pentose phosphate pathway and the glycolysis/gluconeogenesis pathway were up-regulated, which explained the reduction of starch and total soluble sugar content in the leaves of maize seedlings. These findings suggest that phospholipase C plays a key role in photosynthesis and the growth and development of maize seedlings. |
| first_indexed | 2024-03-09T23:43:31Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:43:31Z |
| publishDate | 2022-06-01 |
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| series | Genes |
| spelling | doaj.art-1767b36d2817494fb936bff0789d2b4c2023-11-23T16:47:49ZengMDPI AGGenes2073-44252022-06-01136101110.3390/genes13061011Involvement of Phospholipase C in Photosynthesis and Growth of Maize SeedlingsYulei Wei0Xinyu Liu1Shengnan Ge2Haiyang Zhang3Xinyang Che4Shiyuan Liu5Debin Liu6Huixin Li7Xinru Gu8Lin He9Zuotong Li10Jingyu Xu11Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaKey Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing 163319, ChinaPhospholipase C is an enzyme that catalyzes the hydrolysis of glycerophospholipids and can be classified as phosphoinositide-specific PLC (PI-PLC) and non-specific PLC (NPC), depending on its hydrolytic substrate. In maize, the function of phospholipase C has not been well characterized. In this study, the phospholipase C inhibitor neomycin sulfate (NS, 100 mM) was applied to maize seedlings to investigate the function of maize PLC. Under the treatment of neomycin sulfate, the growth and development of maize seedlings were impaired, and the leaves were gradually etiolated and wilted. The analysis of physiological and biochemical parameters revealed that inhibition of phospholipase C affected photosynthesis, photosynthetic pigment accumulation, carbon metabolism and the stability of the cell membrane. High-throughput RNA-seq was conducted, and differentially expressed genes (DEGS) were found significantly enriched in photosynthesis and carbon metabolism pathways. When phospholipase C activity was inhibited, the expression of genes related to photosynthetic pigment accumulation was decreased, which led to lowered chlorophyll. Most of the genes related to PSI, PSII and TCA cycles were down-regulated and the net photosynthesis was decreased. Meanwhile, genes related to starch and sucrose metabolism, the pentose phosphate pathway and the glycolysis/gluconeogenesis pathway were up-regulated, which explained the reduction of starch and total soluble sugar content in the leaves of maize seedlings. These findings suggest that phospholipase C plays a key role in photosynthesis and the growth and development of maize seedlings.https://www.mdpi.com/2073-4425/13/6/1011maize (<i>Zea mays</i>)phospholipase Cneomycin sulfatephotosynthesisgrowth and development |
| spellingShingle | Yulei Wei Xinyu Liu Shengnan Ge Haiyang Zhang Xinyang Che Shiyuan Liu Debin Liu Huixin Li Xinru Gu Lin He Zuotong Li Jingyu Xu Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings Genes maize (<i>Zea mays</i>) phospholipase C neomycin sulfate photosynthesis growth and development |
| title | Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings |
| title_full | Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings |
| title_fullStr | Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings |
| title_full_unstemmed | Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings |
| title_short | Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings |
| title_sort | involvement of phospholipase c in photosynthesis and growth of maize seedlings |
| topic | maize (<i>Zea mays</i>) phospholipase C neomycin sulfate photosynthesis growth and development |
| url | https://www.mdpi.com/2073-4425/13/6/1011 |
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