Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract
The present research is devoted to optimizing the green synthesis technology of silver nanoparticles (AgNP) with antibacterial properties for further external use. Standardized marigold flowers extract was utilized as reducing and stabilizing agent, which has significantly richer composition of phyt...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
|
| Series: | Results in Surfaces and Interfaces |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666845923000144 |
| _version_ | 1797802696225325056 |
|---|---|
| author | Zhanat Zhangabay Dmitriy Berillo |
| author_facet | Zhanat Zhangabay Dmitriy Berillo |
| author_sort | Zhanat Zhangabay |
| collection | DOAJ |
| description | The present research is devoted to optimizing the green synthesis technology of silver nanoparticles (AgNP) with antibacterial properties for further external use. Standardized marigold flowers extract was utilized as reducing and stabilizing agent, which has significantly richer composition of phytochemical groups compared to its aqueous extracts. A comparative analysis of the physicochemical properties and sedimentation stability of AgNPs obtained by exposure of the reaction mixture to: UV radiation, microwave radiation and high temperature using UV–Vis spectrophotometry and Zeta potential analysis were carried out. pH effect of the reaction mixture on size distribution of AgNPs was evaluated. A good stability of AgNPs to aggregation was observed for samples generated under ultraviolet irradiation with continuous stirring. The particle size distribution obtained by UV irradiation with an average size of 80 nm and PDI 0.26. The storage of AgNPs at 4 °C resulted in some aggregation of small size AgNPs that led to the increase the absorbance at 430 nm. Thus, after one month of storage there was no following increase of the absorbance and there was non-significant aggregation over 3 months’ study. Antibacterial activity of AgNPs against S. aureus and E. coli was estimated with and without light irradiation (445 and 635 nm, 14 W). Additionally, the antioxidant activity of the plant extract and the AgNPs stabilized by the extract was estimated. |
| first_indexed | 2024-03-13T05:09:36Z |
| format | Article |
| id | doaj.art-17698299855744a8918caa2ef8aacfd7 |
| institution | Directory Open Access Journal |
| issn | 2666-8459 |
| language | English |
| last_indexed | 2024-03-13T05:09:36Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Surfaces and Interfaces |
| spelling | doaj.art-17698299855744a8918caa2ef8aacfd72023-06-16T05:12:04ZengElsevierResults in Surfaces and Interfaces2666-84592023-05-0111100109Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extractZhanat Zhangabay0Dmitriy Berillo1Department of Pharmaceutical and Toxicological Chemistry, Pharmacognosy and Botany, Asfendiyarov Kazakh National Medical University, 050000 Almaty, Republic of KazakhstanDepartment of Pharmaceutical and Toxicological Chemistry, Pharmacognosy and Botany, Asfendiyarov Kazakh National Medical University, 050000 Almaty, Republic of Kazakhstan; MINES ParisTech, PSL Research University, Center for Materials Forming (CEMEF), UMR CNRS 7635, CS 10207, 06904 Sophia Antipolis, France; Corresponding author at: Department of Pharmaceutical and Toxicological Chemistry, Pharmacognosy and Botany, Asfendiyarov Kazakh National Medical University, 050000 Almaty, Republic of Kazakhstan.The present research is devoted to optimizing the green synthesis technology of silver nanoparticles (AgNP) with antibacterial properties for further external use. Standardized marigold flowers extract was utilized as reducing and stabilizing agent, which has significantly richer composition of phytochemical groups compared to its aqueous extracts. A comparative analysis of the physicochemical properties and sedimentation stability of AgNPs obtained by exposure of the reaction mixture to: UV radiation, microwave radiation and high temperature using UV–Vis spectrophotometry and Zeta potential analysis were carried out. pH effect of the reaction mixture on size distribution of AgNPs was evaluated. A good stability of AgNPs to aggregation was observed for samples generated under ultraviolet irradiation with continuous stirring. The particle size distribution obtained by UV irradiation with an average size of 80 nm and PDI 0.26. The storage of AgNPs at 4 °C resulted in some aggregation of small size AgNPs that led to the increase the absorbance at 430 nm. Thus, after one month of storage there was no following increase of the absorbance and there was non-significant aggregation over 3 months’ study. Antibacterial activity of AgNPs against S. aureus and E. coli was estimated with and without light irradiation (445 and 635 nm, 14 W). Additionally, the antioxidant activity of the plant extract and the AgNPs stabilized by the extract was estimated.http://www.sciencedirect.com/science/article/pii/S2666845923000144AgNPsPlant extractExtract Calendula officinalisGreen synthesisAntibacterial activityAntioxidant activity |
| spellingShingle | Zhanat Zhangabay Dmitriy Berillo Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract Results in Surfaces and Interfaces AgNPs Plant extract Extract Calendula officinalis Green synthesis Antibacterial activity Antioxidant activity |
| title | Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract |
| title_full | Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract |
| title_fullStr | Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract |
| title_full_unstemmed | Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract |
| title_short | Antimicrobial and antioxidant activity of AgNPs stabilized with Calendula officinalis flower extract |
| title_sort | antimicrobial and antioxidant activity of agnps stabilized with calendula officinalis flower extract |
| topic | AgNPs Plant extract Extract Calendula officinalis Green synthesis Antibacterial activity Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S2666845923000144 |
| work_keys_str_mv | AT zhanatzhangabay antimicrobialandantioxidantactivityofagnpsstabilizedwithcalendulaofficinalisflowerextract AT dmitriyberillo antimicrobialandantioxidantactivityofagnpsstabilizedwithcalendulaofficinalisflowerextract |