Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia
Campylobacteriosis represents a global health challenge due to continuously increasing trends of antimicrobial resistance in <i>Campylobacter jejuni</i>. <i>C. jejuni</i> can sometimes cause life-threatening and severe systematic infections (bacteremia, meningitis, and other...
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1410 |
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| author | Silvija Šoprek Sanja Duvnjak Gordan Kompes Luka Jurinović Arjana Tambić Andrašević |
| author_facet | Silvija Šoprek Sanja Duvnjak Gordan Kompes Luka Jurinović Arjana Tambić Andrašević |
| author_sort | Silvija Šoprek |
| collection | DOAJ |
| description | Campylobacteriosis represents a global health challenge due to continuously increasing trends of antimicrobial resistance in <i>Campylobacter jejuni</i>. <i>C. jejuni</i> can sometimes cause life-threatening and severe systematic infections (bacteremia, meningitis, and other extraintestinal infections) with very few antibiotics left as treatment options. Bearing in mind that <i>C. jejuni</i> is the predominant species in humans, in this paper, we present a study of the <i>C. jejuni</i> differences in antimicrobial resistance and genotype distribution between strains isolated from stool and primary sterile sites. We compared the genomic data obtained through whole genome sequencing (WGS) and phenotypic susceptibility data of <i>C. jejuni</i> strains. Once antimicrobial susceptibility testing of <i>C. jejuni</i> strains was carried out by the broth microdilution method for six of interest, results were compared to the identified genotypic determinants derived from WGS. The high rate of resistance to fluoroquinolones presented in this study is in accordance with national surveillance data. The proportion of strains with acquired resistance was 71% for ciprofloxacin and 20% for tetracycline. When invasive isolates were analysed separately, 40% exhibited MIC values of ciprofloxacin higher than the ECOFFs, suggesting a lower flouroquinolone resistance rate in invasive isolates. All isolates demonstrated wilde-type phenotype for chloramphenicol, erythromycin, gentamicin, and ertapenem. A special focus and review in this study was performed on a group of <i>C.jejuni</i> strains found in primary sterile samples. Apart from demonstrating a lower resistance rate, these isolates seem genetically more uniform, showing epidemiologically more homogenous patterns, which cluster to several clonal complexes, with CC49 being the most represented clonal complex. |
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| language | English |
| last_indexed | 2024-03-09T06:10:04Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-176f02d0a4114f3da0b85d15dfb96c3e2023-12-03T11:59:16ZengMDPI AGMicroorganisms2076-26072022-07-01107141010.3390/microorganisms10071410Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in CroatiaSilvija Šoprek0Sanja Duvnjak1Gordan Kompes2Luka Jurinović3Arjana Tambić Andrašević4Department of Clinical Microbiology, University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, 10000 Zagreb, CroatiaLaboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Department for Bacteriology and Parasitology, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for General Bacteriology and Mycology, Department for Bacteriology and Parasitology, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for Bacteriology, Poultry Centre, Croatian Veterinary Institute, 10000 Zagreb, CroatiaDepartment of Clinical Microbiology, University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, 10000 Zagreb, CroatiaCampylobacteriosis represents a global health challenge due to continuously increasing trends of antimicrobial resistance in <i>Campylobacter jejuni</i>. <i>C. jejuni</i> can sometimes cause life-threatening and severe systematic infections (bacteremia, meningitis, and other extraintestinal infections) with very few antibiotics left as treatment options. Bearing in mind that <i>C. jejuni</i> is the predominant species in humans, in this paper, we present a study of the <i>C. jejuni</i> differences in antimicrobial resistance and genotype distribution between strains isolated from stool and primary sterile sites. We compared the genomic data obtained through whole genome sequencing (WGS) and phenotypic susceptibility data of <i>C. jejuni</i> strains. Once antimicrobial susceptibility testing of <i>C. jejuni</i> strains was carried out by the broth microdilution method for six of interest, results were compared to the identified genotypic determinants derived from WGS. The high rate of resistance to fluoroquinolones presented in this study is in accordance with national surveillance data. The proportion of strains with acquired resistance was 71% for ciprofloxacin and 20% for tetracycline. When invasive isolates were analysed separately, 40% exhibited MIC values of ciprofloxacin higher than the ECOFFs, suggesting a lower flouroquinolone resistance rate in invasive isolates. All isolates demonstrated wilde-type phenotype for chloramphenicol, erythromycin, gentamicin, and ertapenem. A special focus and review in this study was performed on a group of <i>C.jejuni</i> strains found in primary sterile samples. Apart from demonstrating a lower resistance rate, these isolates seem genetically more uniform, showing epidemiologically more homogenous patterns, which cluster to several clonal complexes, with CC49 being the most represented clonal complex.https://www.mdpi.com/2076-2607/10/7/1410<i>Campylobacter jejuni</i>antimicrobial resistanceantimicrobial susceptibility testingbioinformaticswhole genome sequencing<i>Campylobacter jejuni</i> in primary sterile samples |
| spellingShingle | Silvija Šoprek Sanja Duvnjak Gordan Kompes Luka Jurinović Arjana Tambić Andrašević Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia Microorganisms <i>Campylobacter jejuni</i> antimicrobial resistance antimicrobial susceptibility testing bioinformatics whole genome sequencing <i>Campylobacter jejuni</i> in primary sterile samples |
| title | Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia |
| title_full | Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia |
| title_fullStr | Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia |
| title_full_unstemmed | Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia |
| title_short | Resistome Analysis of <i>Campylobacter jejuni</i> Strains Isolated from Human Stool and Primary Sterile Samples in Croatia |
| title_sort | resistome analysis of i campylobacter jejuni i strains isolated from human stool and primary sterile samples in croatia |
| topic | <i>Campylobacter jejuni</i> antimicrobial resistance antimicrobial susceptibility testing bioinformatics whole genome sequencing <i>Campylobacter jejuni</i> in primary sterile samples |
| url | https://www.mdpi.com/2076-2607/10/7/1410 |
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