Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc
Effects of dietary Original XPC (XPC) in commercial layer pullets challenged with the virulent, low passage R strain of Mycoplasma gallisepticum (Rlow MG) were investigated. Hy-Line W-36 pullets sourced from MG-clean breeders were fed a basal diet with or without (CON) XPC (1.25 kg/metric ton) from...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120300936 |
| _version_ | 1818242091688394752 |
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| author | K.E.C. Elliott S.L. Branton J.D. Evans S.A. Leigh E.J. Kim H.A. Olanrewaju G.T. Pharr H.O. Pavlidis P.D. Gerard E.D. Peebles |
| author_facet | K.E.C. Elliott S.L. Branton J.D. Evans S.A. Leigh E.J. Kim H.A. Olanrewaju G.T. Pharr H.O. Pavlidis P.D. Gerard E.D. Peebles |
| author_sort | K.E.C. Elliott |
| collection | DOAJ |
| description | Effects of dietary Original XPC (XPC) in commercial layer pullets challenged with the virulent, low passage R strain of Mycoplasma gallisepticum (Rlow MG) were investigated. Hy-Line W-36 pullets sourced from MG-clean breeders were fed a basal diet with or without (CON) XPC (1.25 kg/metric ton) from hatch until 12 wk of age (woa). At 8 and 10 woa, half of the birds in each dietary treatment were challenged with Rlow MG. Body weight was recorded at 3, 8, and 12 woa, and ovary, ceca, and bursa weights were recorded at 3 and 12 woa. Blood samples were taken immediately before the initial Rlow MG challenge at 8 woa and again at 12 woa to test for IgM and IgG antibody production against MG. All birds were evaluated for MG lesion scores at 12 woa. Regardless of challenge, inclusion of XPC in the diet did not significantly alter BW at 3 or 8 woa or relative organ weights at 3 or 12 woa. However, at 12 woa, BW of XPC-fed birds, regardless of challenge was significantly (P = 0.0038) heavier than CON by 25.7 g. All birds tested negative for MG antibodies before the 8 woa challenge. Respective percentage serum plate agglutination and ELISA positive birds at 12 woa were 0 and 0% (CON, nonchallenged), 1.4 and 0% (XPC, nonchallenged), 100 and 47.2% (CON, challenged), and 100 and 50.0% (XPC, challenged). Diet did not significantly affect ELISA titers, but they were significantly (P < 0.0001) increased due to challenge. Furthermore, lesion scores were significantly higher for Rlow MG-challenged birds (P = 0.0012), and dietary treatment with XPC in challenged birds numerically reduced MG lesion scores from 0.278 to 0.194. In conclusion, although dietary XPC did not significantly alter the humoral immune response, antibody titer levels, or severity of MG lesions in layer pullets that were or were not challenged with Rlow MG, it led to an increase in their rate of growth through 12 woa. |
| first_indexed | 2024-12-12T13:39:43Z |
| format | Article |
| id | doaj.art-176f1270e6104480a93ecd7cefb746f4 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-12T13:39:43Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-176f1270e6104480a93ecd7cefb746f42022-12-22T00:22:50ZengElsevierPoultry Science0032-57912020-06-0199630303037Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabcK.E.C. Elliott0S.L. Branton1J.D. Evans2S.A. Leigh3E.J. Kim4H.A. Olanrewaju5G.T. Pharr6H.O. Pavlidis7P.D. Gerard8E.D. Peebles9Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USAUSDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, USAUSDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, USAUSDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, USAUSDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, USAUSDA-ARS, Poultry Research Unit, Mississippi State, MS 39762, USADepartment of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USADiamond V Corp., Cedar Rapids, IA 52404, USASchool of Mathematical and Statistical Sciences, Clemson University, Clemson, SC 29634, USADepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA; Corresponding author:Effects of dietary Original XPC (XPC) in commercial layer pullets challenged with the virulent, low passage R strain of Mycoplasma gallisepticum (Rlow MG) were investigated. Hy-Line W-36 pullets sourced from MG-clean breeders were fed a basal diet with or without (CON) XPC (1.25 kg/metric ton) from hatch until 12 wk of age (woa). At 8 and 10 woa, half of the birds in each dietary treatment were challenged with Rlow MG. Body weight was recorded at 3, 8, and 12 woa, and ovary, ceca, and bursa weights were recorded at 3 and 12 woa. Blood samples were taken immediately before the initial Rlow MG challenge at 8 woa and again at 12 woa to test for IgM and IgG antibody production against MG. All birds were evaluated for MG lesion scores at 12 woa. Regardless of challenge, inclusion of XPC in the diet did not significantly alter BW at 3 or 8 woa or relative organ weights at 3 or 12 woa. However, at 12 woa, BW of XPC-fed birds, regardless of challenge was significantly (P = 0.0038) heavier than CON by 25.7 g. All birds tested negative for MG antibodies before the 8 woa challenge. Respective percentage serum plate agglutination and ELISA positive birds at 12 woa were 0 and 0% (CON, nonchallenged), 1.4 and 0% (XPC, nonchallenged), 100 and 47.2% (CON, challenged), and 100 and 50.0% (XPC, challenged). Diet did not significantly affect ELISA titers, but they were significantly (P < 0.0001) increased due to challenge. Furthermore, lesion scores were significantly higher for Rlow MG-challenged birds (P = 0.0012), and dietary treatment with XPC in challenged birds numerically reduced MG lesion scores from 0.278 to 0.194. In conclusion, although dietary XPC did not significantly alter the humoral immune response, antibody titer levels, or severity of MG lesions in layer pullets that were or were not challenged with Rlow MG, it led to an increase in their rate of growth through 12 woa.http://www.sciencedirect.com/science/article/pii/S0032579120300936growthimmunitylayer pulletsMycoplasma gallisepticumOriginal XPC |
| spellingShingle | K.E.C. Elliott S.L. Branton J.D. Evans S.A. Leigh E.J. Kim H.A. Olanrewaju G.T. Pharr H.O. Pavlidis P.D. Gerard E.D. Peebles Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc Poultry Science growth immunity layer pullets Mycoplasma gallisepticum Original XPC |
| title | Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc |
| title_full | Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc |
| title_fullStr | Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc |
| title_full_unstemmed | Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc |
| title_short | Growth and humoral immune effects of dietary Original XPC in layer pullets challenged with Mycoplasma gallisepticumabc |
| title_sort | growth and humoral immune effects of dietary original xpc in layer pullets challenged with mycoplasma gallisepticumabc |
| topic | growth immunity layer pullets Mycoplasma gallisepticum Original XPC |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120300936 |
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