Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells
Porphyromonas gingivalis (Pg) is a periodontopathic pathogen that may affect MUC5AC‐related mucus hypersecretion along airway epithelial cells. Here, we attempted to establish whether Pg virulence factors (lipopolysaccharide, FimA fimbriae, gingipains) affect MUC5AC in immortalized and primary bronc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-02-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13066 |
| _version_ | 1811178369181548544 |
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| author | Chihiro Miya Marni E. Cueno Ryuta Suzuki Shuichiro Maruoka Yasuhiro Gon Tadayoshi Kaneko Yoshiyuki Yonehara Kenichi Imai |
| author_facet | Chihiro Miya Marni E. Cueno Ryuta Suzuki Shuichiro Maruoka Yasuhiro Gon Tadayoshi Kaneko Yoshiyuki Yonehara Kenichi Imai |
| author_sort | Chihiro Miya |
| collection | DOAJ |
| description | Porphyromonas gingivalis (Pg) is a periodontopathic pathogen that may affect MUC5AC‐related mucus hypersecretion along airway epithelial cells. Here, we attempted to establish whether Pg virulence factors (lipopolysaccharide, FimA fimbriae, gingipains) affect MUC5AC in immortalized and primary bronchial cells. We report that MUC5AC gene expression and protein levels are affected by Pg culture supernatant, but not by lipopolysaccharide or FimA fimbriae. Cells treated with either Pg single (Kgp or Rgp) or double (Kgp/Rgp) mutants had altered levels of MUC5AC gene expression and protein levels, and MUC5AC staining of double mutant‐treated mouse lung cells showed that MUC5AC protein levels were unaffected. Taken together, we propose that Pg gingipains may be the primary virulence factor that influences both MUC5AC gene expression and protein levels. |
| first_indexed | 2024-04-11T06:17:04Z |
| format | Article |
| id | doaj.art-176fd0caf75549cfb45cb0a6177a8852 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T06:17:04Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-176fd0caf75549cfb45cb0a6177a88522022-12-22T04:41:01ZengWileyFEBS Open Bio2211-54632021-02-0111244645510.1002/2211-5463.13066Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cellsChihiro Miya0Marni E. Cueno1Ryuta Suzuki2Shuichiro Maruoka3Yasuhiro Gon4Tadayoshi Kaneko5Yoshiyuki Yonehara6Kenichi Imai7Department of Oral and Maxillofacial Surgery II Nihon University School of Dentistry Tokyo JapanDepartment of Microbiology Nihon University School of Dentistry Tokyo JapanDepartment of Oral and Maxillofacial Surgery II Nihon University School of Dentistry Tokyo JapanDepartment of Internal Medicine Nihon University School of Medicine Tokyo JapanDepartment of Internal Medicine Nihon University School of Medicine Tokyo JapanDepartment of Oral and Maxillofacial Surgery II Nihon University School of Dentistry Tokyo JapanDepartment of Oral and Maxillofacial Surgery II Nihon University School of Dentistry Tokyo JapanDepartment of Microbiology Nihon University School of Dentistry Tokyo JapanPorphyromonas gingivalis (Pg) is a periodontopathic pathogen that may affect MUC5AC‐related mucus hypersecretion along airway epithelial cells. Here, we attempted to establish whether Pg virulence factors (lipopolysaccharide, FimA fimbriae, gingipains) affect MUC5AC in immortalized and primary bronchial cells. We report that MUC5AC gene expression and protein levels are affected by Pg culture supernatant, but not by lipopolysaccharide or FimA fimbriae. Cells treated with either Pg single (Kgp or Rgp) or double (Kgp/Rgp) mutants had altered levels of MUC5AC gene expression and protein levels, and MUC5AC staining of double mutant‐treated mouse lung cells showed that MUC5AC protein levels were unaffected. Taken together, we propose that Pg gingipains may be the primary virulence factor that influences both MUC5AC gene expression and protein levels.https://doi.org/10.1002/2211-5463.13066epithelial cellgingipainsimmortalized cellsMUC5ACPorphyromonas gingivalisprimary bronchial cells |
| spellingShingle | Chihiro Miya Marni E. Cueno Ryuta Suzuki Shuichiro Maruoka Yasuhiro Gon Tadayoshi Kaneko Yoshiyuki Yonehara Kenichi Imai Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells FEBS Open Bio epithelial cell gingipains immortalized cells MUC5AC Porphyromonas gingivalis primary bronchial cells |
| title | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells |
| title_full | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells |
| title_fullStr | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells |
| title_full_unstemmed | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells |
| title_short | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells |
| title_sort | porphyromonas gingivalis gingipains potentially affect muc5ac gene expression and protein levels in respiratory epithelial cells |
| topic | epithelial cell gingipains immortalized cells MUC5AC Porphyromonas gingivalis primary bronchial cells |
| url | https://doi.org/10.1002/2211-5463.13066 |
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