Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation
Because low back pain is frequently a result of intervertebral disc degeneration (IVDD), strategies to regenerate or repair the IVD are currently being investigated. Often, ex vivo disc cultures of non-human IVD organs or tissue explants are used that usually do not exhibit natural IVDD. Therefore,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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AO Research Institute Davos
2023-03-01
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| Series: | European Cells & Materials |
| Subjects: | |
| Online Access: | https://www.ecmjournal.org/papers/vol045/pdf/v045a07.pdf |
| _version_ | 1797857397928099840 |
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| author | E Salzer TC Schmitz VHM Mouser A Vernengo B Gantenbein JU Jansen C Neidlinger-Wilke H-J Wilke S Grad CL Le Maitre MA Tryfonidou K Ito |
| author_facet | E Salzer TC Schmitz VHM Mouser A Vernengo B Gantenbein JU Jansen C Neidlinger-Wilke H-J Wilke S Grad CL Le Maitre MA Tryfonidou K Ito |
| author_sort | E Salzer |
| collection | DOAJ |
| description | Because low back pain is frequently a result of intervertebral disc degeneration (IVDD), strategies to regenerate or repair the IVD are currently being investigated. Often, ex vivo disc cultures of non-human IVD organs or tissue explants are used that usually do not exhibit natural IVDD. Therefore, degenerative changes mimicking those reported in human IVDD need to be induced. To support researchers in selecting ex vivo disc cultures, a systematic search was performed for them and their potential use for studying human IVDD reviewed. Five degeneration induction categories (proinflammatory cytokines, injury/damage, degenerative loading, enzyme, and other) were identified in 129 studies across 7 species. Methods to induce degeneration are diverse and can induce mild to severe degenerative changes that progress over time, as described for human IVDD. The induced degenerative changes are model-specific and there is no “one-fits-all” IVDD induction method. Nevertheless, specific aspects of human IVDD can be well mimicked. Currently, spontaneously degenerated disc cultures from large animals capture human IVDD in most aspects. Combinatorial approaches of several induction methods using discs derived from large animals are promising to recapitulate pathological changes on several levels, such as cellular behaviour, extracellular matrix composition, and biomechanical function, and therefore better mimic human IVDD. Future disc culture setups might increase in complexity, and mimic human IVDD even better. As ex vivo disc cultures have the potential to reduce and even replace animal trials, especially during preclinical development, advancement of such models is highly relevant for more efficient and cost-effective clinical translation from bench-to-bedside. |
| first_indexed | 2024-04-09T20:56:03Z |
| format | Article |
| id | doaj.art-177001c7eb6f4e5fa3ce5bb1226e65be |
| institution | Directory Open Access Journal |
| issn | 1473-2262 |
| language | English |
| last_indexed | 2024-04-09T20:56:03Z |
| publishDate | 2023-03-01 |
| publisher | AO Research Institute Davos |
| record_format | Article |
| series | European Cells & Materials |
| spelling | doaj.art-177001c7eb6f4e5fa3ce5bb1226e65be2023-03-29T16:23:30ZengAO Research Institute DavosEuropean Cells & Materials1473-22622023-03-01458811210.22203/eCM.v045a07Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translationE SalzerTC SchmitzVHM MouserA VernengoB GantenbeinJU JansenC Neidlinger-WilkeH-J WilkeS GradCL Le MaitreMA Tryfonidou K ItoBecause low back pain is frequently a result of intervertebral disc degeneration (IVDD), strategies to regenerate or repair the IVD are currently being investigated. Often, ex vivo disc cultures of non-human IVD organs or tissue explants are used that usually do not exhibit natural IVDD. Therefore, degenerative changes mimicking those reported in human IVDD need to be induced. To support researchers in selecting ex vivo disc cultures, a systematic search was performed for them and their potential use for studying human IVDD reviewed. Five degeneration induction categories (proinflammatory cytokines, injury/damage, degenerative loading, enzyme, and other) were identified in 129 studies across 7 species. Methods to induce degeneration are diverse and can induce mild to severe degenerative changes that progress over time, as described for human IVDD. The induced degenerative changes are model-specific and there is no “one-fits-all” IVDD induction method. Nevertheless, specific aspects of human IVDD can be well mimicked. Currently, spontaneously degenerated disc cultures from large animals capture human IVDD in most aspects. Combinatorial approaches of several induction methods using discs derived from large animals are promising to recapitulate pathological changes on several levels, such as cellular behaviour, extracellular matrix composition, and biomechanical function, and therefore better mimic human IVDD. Future disc culture setups might increase in complexity, and mimic human IVDD even better. As ex vivo disc cultures have the potential to reduce and even replace animal trials, especially during preclinical development, advancement of such models is highly relevant for more efficient and cost-effective clinical translation from bench-to-bedside.https://www.ecmjournal.org/papers/vol045/pdf/v045a07.pdfdisc cultureorgan cultureexplant culture3rlow back pain |
| spellingShingle | E Salzer TC Schmitz VHM Mouser A Vernengo B Gantenbein JU Jansen C Neidlinger-Wilke H-J Wilke S Grad CL Le Maitre MA Tryfonidou K Ito Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation European Cells & Materials disc culture organ culture explant culture 3r low back pain |
| title | Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation |
| title_full | Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation |
| title_fullStr | Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation |
| title_full_unstemmed | Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation |
| title_short | Ex vivo intervertebral disc cultures: degeneration-induction methods and their implications for clinical translation |
| title_sort | ex vivo intervertebral disc cultures degeneration induction methods and their implications for clinical translation |
| topic | disc culture organ culture explant culture 3r low back pain |
| url | https://www.ecmjournal.org/papers/vol045/pdf/v045a07.pdf |
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