Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes
Cells adhered to an external solid substrate are observed to exhibit rich dynamics of actin structures on the basal membrane, which are distinct from those observed on the dorsal (free) membrane. Here we explore the dynamics of curved membrane proteins, or protein complexes, that recruit actin polym...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/3/782 |
| _version_ | 1797711287568826368 |
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| author | Moshe Naoz Nir S. Gov |
| author_facet | Moshe Naoz Nir S. Gov |
| author_sort | Moshe Naoz |
| collection | DOAJ |
| description | Cells adhered to an external solid substrate are observed to exhibit rich dynamics of actin structures on the basal membrane, which are distinct from those observed on the dorsal (free) membrane. Here we explore the dynamics of curved membrane proteins, or protein complexes, that recruit actin polymerization when the membrane is confined by the solid substrate. Such curved proteins can induce the spontaneous formation of membrane protrusions on the dorsal side of cells. However, on the basal side of the cells, such protrusions can only extend as far as the solid substrate and this constraint can convert such protrusions into propagating wave-like structures. We also demonstrate that adhesion molecules can stabilize localized protrusions that resemble some features of podosomes. This coupling of curvature and actin forces may underlie the differences in the observed actin-membrane dynamics between the basal and dorsal sides of adhered cells. |
| first_indexed | 2024-03-12T07:04:46Z |
| format | Article |
| id | doaj.art-1772691342344f9b9e6b042f10f116e9 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T07:04:46Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1772691342344f9b9e6b042f10f116e92023-09-02T23:28:11ZengMDPI AGCells2073-44092020-03-019378210.3390/cells9030782cells9030782Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and PodosomesMoshe Naoz0Nir S. Gov1Department of Chemical and Biological Physics, Weizmann Institute of Science, P.O.B. 26, Rehovot 76100, IsraelDepartment of Chemical and Biological Physics, Weizmann Institute of Science, P.O.B. 26, Rehovot 76100, IsraelCells adhered to an external solid substrate are observed to exhibit rich dynamics of actin structures on the basal membrane, which are distinct from those observed on the dorsal (free) membrane. Here we explore the dynamics of curved membrane proteins, or protein complexes, that recruit actin polymerization when the membrane is confined by the solid substrate. Such curved proteins can induce the spontaneous formation of membrane protrusions on the dorsal side of cells. However, on the basal side of the cells, such protrusions can only extend as far as the solid substrate and this constraint can convert such protrusions into propagating wave-like structures. We also demonstrate that adhesion molecules can stabilize localized protrusions that resemble some features of podosomes. This coupling of curvature and actin forces may underlie the differences in the observed actin-membrane dynamics between the basal and dorsal sides of adhered cells.https://www.mdpi.com/2073-4409/9/3/782actin wavescurved proteinsdynamic instabilitypodosomes |
| spellingShingle | Moshe Naoz Nir S. Gov Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes Cells actin waves curved proteins dynamic instability podosomes |
| title | Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes |
| title_full | Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes |
| title_fullStr | Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes |
| title_full_unstemmed | Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes |
| title_short | Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes |
| title_sort | cell substrate patterns driven by curvature sensitive actin polymerization waves and podosomes |
| topic | actin waves curved proteins dynamic instability podosomes |
| url | https://www.mdpi.com/2073-4409/9/3/782 |
| work_keys_str_mv | AT moshenaoz cellsubstratepatternsdrivenbycurvaturesensitiveactinpolymerizationwavesandpodosomes AT nirsgov cellsubstratepatternsdrivenbycurvaturesensitiveactinpolymerizationwavesandpodosomes |