Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).
We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differenti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171228&type=printable |
| _version_ | 1826579294877384704 |
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| author | Zachary F Phillips Michael Chen Laura Waller |
| author_facet | Zachary F Phillips Michael Chen Laura Waller |
| author_sort | Zachary F Phillips |
| collection | DOAJ |
| description | We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel. |
| first_indexed | 2024-12-10T16:19:55Z |
| format | Article |
| id | doaj.art-1773839afa824d64b68f6d1203dddb80 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T14:16:00Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1773839afa824d64b68f6d1203dddb802025-02-27T05:33:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017122810.1371/journal.pone.0171228Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).Zachary F PhillipsMichael ChenLaura WallerWe present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171228&type=printable |
| spellingShingle | Zachary F Phillips Michael Chen Laura Waller Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). PLoS ONE |
| title | Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). |
| title_full | Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). |
| title_fullStr | Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). |
| title_full_unstemmed | Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). |
| title_short | Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC). |
| title_sort | single shot quantitative phase microscopy with color multiplexed differential phase contrast cdpc |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171228&type=printable |
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