Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes
Legumes are the recommended crops to fight against soil degradation and loss of fertility because of their known positive impacts on soils. Our interest is focused on the identification of plant-growth-promoting endophytes inhabiting nodules able to enhance legume growth in poor and/or degraded soil...
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/8/1091 |
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| author | Noris J. Flores-Duarte Julia Pérez-Pérez Salvadora Navarro-Torre Enrique Mateos-Naranjo Susana Redondo-Gómez Eloísa Pajuelo Ignacio D. Rodríguez-Llorente |
| author_facet | Noris J. Flores-Duarte Julia Pérez-Pérez Salvadora Navarro-Torre Enrique Mateos-Naranjo Susana Redondo-Gómez Eloísa Pajuelo Ignacio D. Rodríguez-Llorente |
| author_sort | Noris J. Flores-Duarte |
| collection | DOAJ |
| description | Legumes are the recommended crops to fight against soil degradation and loss of fertility because of their known positive impacts on soils. Our interest is focused on the identification of plant-growth-promoting endophytes inhabiting nodules able to enhance legume growth in poor and/or degraded soils. The ability of <i>Variovorax paradoxus</i> S110<sup>T</sup> and <i>Variovorax gossypii</i> JM-310<sup>T</sup> to promote alfalfa growth in nutrient-poor and metal-contaminated estuarine soils was studied. Both strains behaved as nodule endophytes and improved in vitro seed germination and plant growth, as well as nodulation in co-inoculation with <i>Ensifer medicae</i> MA11. <i>Variovorax</i> ameliorated the physiological status of the plant, increased nodulation, chlorophyll and nitrogen content, and the response to stress and metal accumulation in the roots of alfalfa growing in degraded soils with moderate to high levels of contamination. The presence of plant-growth-promoting traits in <i>Variovorax</i>, particularly ACC deaminase activity, could be under the observed in planta effects. Although the couple <i>V. gossypii</i>-MA11 reported a great benefit to plant growth and nodulation, the best result was observed in plants inoculated with the combination of the three bacteria. These results suggest that <i>Variovorax</i> strains could be used as biofertilizers to improve the adaptation of legumes to degraded soils in soil-recovery programs. |
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| format | Article |
| id | doaj.art-1773a4b8e14b4866a3d42b64dc208cb0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T04:16:43Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1773a4b8e14b4866a3d42b64dc208cb02023-12-03T13:52:07ZengMDPI AGPlants2223-77472022-04-01118109110.3390/plants11081091Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. EndophytesNoris J. Flores-Duarte0Julia Pérez-Pérez1Salvadora Navarro-Torre2Enrique Mateos-Naranjo3Susana Redondo-Gómez4Eloísa Pajuelo5Ignacio D. Rodríguez-Llorente6Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, SpainLegumes are the recommended crops to fight against soil degradation and loss of fertility because of their known positive impacts on soils. Our interest is focused on the identification of plant-growth-promoting endophytes inhabiting nodules able to enhance legume growth in poor and/or degraded soils. The ability of <i>Variovorax paradoxus</i> S110<sup>T</sup> and <i>Variovorax gossypii</i> JM-310<sup>T</sup> to promote alfalfa growth in nutrient-poor and metal-contaminated estuarine soils was studied. Both strains behaved as nodule endophytes and improved in vitro seed germination and plant growth, as well as nodulation in co-inoculation with <i>Ensifer medicae</i> MA11. <i>Variovorax</i> ameliorated the physiological status of the plant, increased nodulation, chlorophyll and nitrogen content, and the response to stress and metal accumulation in the roots of alfalfa growing in degraded soils with moderate to high levels of contamination. The presence of plant-growth-promoting traits in <i>Variovorax</i>, particularly ACC deaminase activity, could be under the observed in planta effects. Although the couple <i>V. gossypii</i>-MA11 reported a great benefit to plant growth and nodulation, the best result was observed in plants inoculated with the combination of the three bacteria. These results suggest that <i>Variovorax</i> strains could be used as biofertilizers to improve the adaptation of legumes to degraded soils in soil-recovery programs.https://www.mdpi.com/2223-7747/11/8/1091ACC deaminaselegume nodulationplant-growth-promoting endophytesdegraded soils |
| spellingShingle | Noris J. Flores-Duarte Julia Pérez-Pérez Salvadora Navarro-Torre Enrique Mateos-Naranjo Susana Redondo-Gómez Eloísa Pajuelo Ignacio D. Rodríguez-Llorente Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes Plants ACC deaminase legume nodulation plant-growth-promoting endophytes degraded soils |
| title | Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes |
| title_full | Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes |
| title_fullStr | Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes |
| title_full_unstemmed | Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes |
| title_short | Improved <i>Medicago sativa</i> Nodulation under Stress Assisted by <i>Variovorax</i> sp. Endophytes |
| title_sort | improved i medicago sativa i nodulation under stress assisted by i variovorax i sp endophytes |
| topic | ACC deaminase legume nodulation plant-growth-promoting endophytes degraded soils |
| url | https://www.mdpi.com/2223-7747/11/8/1091 |
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