On-site applicable diagnostic fluorescent probe for fire blight bacteria
Summary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant...
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Format: | Article |
Language: | English |
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Elsevier
2023-04-01
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Series: | iScience |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422300634X |
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author | Yuna Jung Ji Hye Jin Minji Jung In Sun Hwang Nguyen Trung Vu Yoon Kyung Park Na Hee Kim Ki-Ho Park Chang-Sik Oh Dokyoung Kim |
author_facet | Yuna Jung Ji Hye Jin Minji Jung In Sun Hwang Nguyen Trung Vu Yoon Kyung Park Na Hee Kim Ki-Ho Park Chang-Sik Oh Dokyoung Kim |
author_sort | Yuna Jung |
collection | DOAJ |
description | Summary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant organs. We newly disclose the fluorogenic probe B-1 for real-time on-site detection of fire blight bacteria for the first time. B-1 exhibited no emission signals but manifested bright emission properties in the presence of fire blight bacteria. Based on these features, fluorescence imaging of the fire blight bacteria and its real-time detection from the infected host plant tissues were conducted. The detection limit against E. amylovora was 102 CFU/mL, which had excellent sensitivity. The fluorogenic probe-based on-site diagnostic technology was supplemented by introducing a new portable UV device. This work holds enormous potential to be a new advanced tool for detecting fire blight in agricultural and livestock industries. |
first_indexed | 2024-04-09T18:15:18Z |
format | Article |
id | doaj.art-1777376fb77942859adc6dfc6869db5b |
institution | Directory Open Access Journal |
issn | 2589-0042 |
language | English |
last_indexed | 2024-04-09T18:15:18Z |
publishDate | 2023-04-01 |
publisher | Elsevier |
record_format | Article |
series | iScience |
spelling | doaj.art-1777376fb77942859adc6dfc6869db5b2023-04-13T04:27:02ZengElsevieriScience2589-00422023-04-01264106557On-site applicable diagnostic fluorescent probe for fire blight bacteriaYuna Jung0Ji Hye Jin1Minji Jung2In Sun Hwang3Nguyen Trung Vu4Yoon Kyung Park5Na Hee Kim6Ki-Ho Park7Chang-Sik Oh8Dokyoung Kim9Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaMedical Science Research Institute, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul 02447, Republic of Korea; Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea; UC San Diego Materials Research Science and Engineering Center, 9500 Gilman Drive, La Jolla, CA 92093, USA; Corresponding authorSummary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant organs. We newly disclose the fluorogenic probe B-1 for real-time on-site detection of fire blight bacteria for the first time. B-1 exhibited no emission signals but manifested bright emission properties in the presence of fire blight bacteria. Based on these features, fluorescence imaging of the fire blight bacteria and its real-time detection from the infected host plant tissues were conducted. The detection limit against E. amylovora was 102 CFU/mL, which had excellent sensitivity. The fluorogenic probe-based on-site diagnostic technology was supplemented by introducing a new portable UV device. This work holds enormous potential to be a new advanced tool for detecting fire blight in agricultural and livestock industries.http://www.sciencedirect.com/science/article/pii/S258900422300634XMicrobiologyPlant biotechnologyPlant pathology |
spellingShingle | Yuna Jung Ji Hye Jin Minji Jung In Sun Hwang Nguyen Trung Vu Yoon Kyung Park Na Hee Kim Ki-Ho Park Chang-Sik Oh Dokyoung Kim On-site applicable diagnostic fluorescent probe for fire blight bacteria iScience Microbiology Plant biotechnology Plant pathology |
title | On-site applicable diagnostic fluorescent probe for fire blight bacteria |
title_full | On-site applicable diagnostic fluorescent probe for fire blight bacteria |
title_fullStr | On-site applicable diagnostic fluorescent probe for fire blight bacteria |
title_full_unstemmed | On-site applicable diagnostic fluorescent probe for fire blight bacteria |
title_short | On-site applicable diagnostic fluorescent probe for fire blight bacteria |
title_sort | on site applicable diagnostic fluorescent probe for fire blight bacteria |
topic | Microbiology Plant biotechnology Plant pathology |
url | http://www.sciencedirect.com/science/article/pii/S258900422300634X |
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