On-site applicable diagnostic fluorescent probe for fire blight bacteria

Summary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant...

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Main Authors: Yuna Jung, Ji Hye Jin, Minji Jung, In Sun Hwang, Nguyen Trung Vu, Yoon Kyung Park, Na Hee Kim, Ki-Ho Park, Chang-Sik Oh, Dokyoung Kim
Format: Article
Language:English
Published: Elsevier 2023-04-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S258900422300634X
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author Yuna Jung
Ji Hye Jin
Minji Jung
In Sun Hwang
Nguyen Trung Vu
Yoon Kyung Park
Na Hee Kim
Ki-Ho Park
Chang-Sik Oh
Dokyoung Kim
author_facet Yuna Jung
Ji Hye Jin
Minji Jung
In Sun Hwang
Nguyen Trung Vu
Yoon Kyung Park
Na Hee Kim
Ki-Ho Park
Chang-Sik Oh
Dokyoung Kim
author_sort Yuna Jung
collection DOAJ
description Summary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant organs. We newly disclose the fluorogenic probe B-1 for real-time on-site detection of fire blight bacteria for the first time. B-1 exhibited no emission signals but manifested bright emission properties in the presence of fire blight bacteria. Based on these features, fluorescence imaging of the fire blight bacteria and its real-time detection from the infected host plant tissues were conducted. The detection limit against E. amylovora was 102 CFU/mL, which had excellent sensitivity. The fluorogenic probe-based on-site diagnostic technology was supplemented by introducing a new portable UV device. This work holds enormous potential to be a new advanced tool for detecting fire blight in agricultural and livestock industries.
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spelling doaj.art-1777376fb77942859adc6dfc6869db5b2023-04-13T04:27:02ZengElsevieriScience2589-00422023-04-01264106557On-site applicable diagnostic fluorescent probe for fire blight bacteriaYuna Jung0Ji Hye Jin1Minji Jung2In Sun Hwang3Nguyen Trung Vu4Yoon Kyung Park5Na Hee Kim6Ki-Ho Park7Chang-Sik Oh8Dokyoung Kim9Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaMedical Science Research Institute, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul 02447, Republic of Korea; Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea; UC San Diego Materials Research Science and Engineering Center, 9500 Gilman Drive, La Jolla, CA 92093, USA; Corresponding authorSummary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant organs. We newly disclose the fluorogenic probe B-1 for real-time on-site detection of fire blight bacteria for the first time. B-1 exhibited no emission signals but manifested bright emission properties in the presence of fire blight bacteria. Based on these features, fluorescence imaging of the fire blight bacteria and its real-time detection from the infected host plant tissues were conducted. The detection limit against E. amylovora was 102 CFU/mL, which had excellent sensitivity. The fluorogenic probe-based on-site diagnostic technology was supplemented by introducing a new portable UV device. This work holds enormous potential to be a new advanced tool for detecting fire blight in agricultural and livestock industries.http://www.sciencedirect.com/science/article/pii/S258900422300634XMicrobiologyPlant biotechnologyPlant pathology
spellingShingle Yuna Jung
Ji Hye Jin
Minji Jung
In Sun Hwang
Nguyen Trung Vu
Yoon Kyung Park
Na Hee Kim
Ki-Ho Park
Chang-Sik Oh
Dokyoung Kim
On-site applicable diagnostic fluorescent probe for fire blight bacteria
iScience
Microbiology
Plant biotechnology
Plant pathology
title On-site applicable diagnostic fluorescent probe for fire blight bacteria
title_full On-site applicable diagnostic fluorescent probe for fire blight bacteria
title_fullStr On-site applicable diagnostic fluorescent probe for fire blight bacteria
title_full_unstemmed On-site applicable diagnostic fluorescent probe for fire blight bacteria
title_short On-site applicable diagnostic fluorescent probe for fire blight bacteria
title_sort on site applicable diagnostic fluorescent probe for fire blight bacteria
topic Microbiology
Plant biotechnology
Plant pathology
url http://www.sciencedirect.com/science/article/pii/S258900422300634X
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