Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status
Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retrieve...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-06-01
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| Series: | Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/jvetres-2019-0025 |
| _version_ | 1819007230094082048 |
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| author | Domosławska Anna Zduńczyk Sławomir Janowski Tomasz |
| author_facet | Domosławska Anna Zduńczyk Sławomir Janowski Tomasz |
| author_sort | Domosławska Anna |
| collection | DOAJ |
| description | Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status. |
| first_indexed | 2024-12-21T00:21:16Z |
| format | Article |
| id | doaj.art-1778ef74a097410aa6678f896827a8ab |
| institution | Directory Open Access Journal |
| issn | 2450-8608 |
| language | English |
| last_indexed | 2024-12-21T00:21:16Z |
| publishDate | 2019-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Veterinary Research |
| spelling | doaj.art-1778ef74a097410aa6678f896827a8ab2022-12-21T19:22:05ZengSciendoJournal of Veterinary Research2450-86082019-06-0163229329710.2478/jvetres-2019-0025jvetres-2019-0025Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium statusDomosławska Anna0Zduńczyk Sławomir1Janowski Tomasz2Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719Olsztyn, PolandDepartment of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719Olsztyn, PolandDepartment of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719Olsztyn, PolandIntroduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.https://doi.org/10.2478/jvetres-2019-0025dogseleniumvitamin esemenfertility |
| spellingShingle | Domosławska Anna Zduńczyk Sławomir Janowski Tomasz Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status Journal of Veterinary Research dog selenium vitamin e semen fertility |
| title | Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status |
| title_full | Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status |
| title_fullStr | Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status |
| title_full_unstemmed | Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status |
| title_short | Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status |
| title_sort | improvement of sperm motility within one month under selenium and vitamin e supplementation in four infertile dogs with low selenium status |
| topic | dog selenium vitamin e semen fertility |
| url | https://doi.org/10.2478/jvetres-2019-0025 |
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