Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection
<i>Phakopsora pachyrhizi</i> is a biotrophic fungus, causer of the disease Asian Soybean Rust, a severe crop disease of soybean and one that demands greater investment from producers. Thus, research efforts to control this disease are still needed. We investigated the expression of metab...
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MDPI AG
2021-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/3/179 |
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| author | Evandro Silva José Perez da Graça Carla Porto Rodolpho Martin do Prado Estela Nunes Francismar Corrêa Marcelino-Guimarães Mauricio Conrado Meyer Eduardo Jorge Pilau |
| author_facet | Evandro Silva José Perez da Graça Carla Porto Rodolpho Martin do Prado Estela Nunes Francismar Corrêa Marcelino-Guimarães Mauricio Conrado Meyer Eduardo Jorge Pilau |
| author_sort | Evandro Silva |
| collection | DOAJ |
| description | <i>Phakopsora pachyrhizi</i> is a biotrophic fungus, causer of the disease Asian Soybean Rust, a severe crop disease of soybean and one that demands greater investment from producers. Thus, research efforts to control this disease are still needed. We investigated the expression of metabolites in soybean plants presenting a resistant genotype inoculated with <i>P. pachyrhizi</i> through the untargeted metabolomics approach. The analysis was performed in control and inoculated plants with <i>P. pachyrhizi</i> using UHPLC-MS/MS. Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA), was applied to the data analysis. PCA and PLS-DA resulted in a clear separation and classification of groups between control and inoculated plants. The metabolites were putative classified and identified using the Global Natural Products Social Molecular Networking platform in flavonoids, isoflavonoids, lipids, fatty acyls, terpenes, and carboxylic acids. Flavonoids and isoflavonoids were up-regulation, while terpenes were down-regulated in response to the soybean–<i>P. pachyrhizi</i> interaction. Our data provide insights into the potential role of some metabolites as flavonoids and isoflavonoids in the plant resistance to ASR. This information could result in the development of resistant genotypes of soybean to <i>P. pachyrhizi</i>, and effective and specific products against the pathogen. |
| first_indexed | 2024-03-10T13:05:28Z |
| format | Article |
| id | doaj.art-177bf6ce707b44c2bd3f86147986fafc |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T13:05:28Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-177bf6ce707b44c2bd3f86147986fafc2023-11-21T11:06:32ZengMDPI AGMetabolites2218-19892021-03-0111317910.3390/metabo11030179Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> InfectionEvandro Silva0José Perez da Graça1Carla Porto2Rodolpho Martin do Prado3Estela Nunes4Francismar Corrêa Marcelino-Guimarães5Mauricio Conrado Meyer6Eduardo Jorge Pilau7Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá 87020-080, PR, BrazilBrazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina 86001-970, PR, BrazilLaboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá 87020-080, PR, BrazilLaboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá 87020-080, PR, BrazilBrazilian Agricultural Research Corporation Swine & Poultry, BR-153, Km 110 Rd, Concórdia 89715-899, SC, BrazilBrazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina 86001-970, PR, BrazilBrazilian Agricultural Research Corporation Soybean, Carlos João Strass Rd, Londrina 86001-970, PR, BrazilLaboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, 5790, Colombo Av, Maringá 87020-080, PR, Brazil<i>Phakopsora pachyrhizi</i> is a biotrophic fungus, causer of the disease Asian Soybean Rust, a severe crop disease of soybean and one that demands greater investment from producers. Thus, research efforts to control this disease are still needed. We investigated the expression of metabolites in soybean plants presenting a resistant genotype inoculated with <i>P. pachyrhizi</i> through the untargeted metabolomics approach. The analysis was performed in control and inoculated plants with <i>P. pachyrhizi</i> using UHPLC-MS/MS. Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA), was applied to the data analysis. PCA and PLS-DA resulted in a clear separation and classification of groups between control and inoculated plants. The metabolites were putative classified and identified using the Global Natural Products Social Molecular Networking platform in flavonoids, isoflavonoids, lipids, fatty acyls, terpenes, and carboxylic acids. Flavonoids and isoflavonoids were up-regulation, while terpenes were down-regulated in response to the soybean–<i>P. pachyrhizi</i> interaction. Our data provide insights into the potential role of some metabolites as flavonoids and isoflavonoids in the plant resistance to ASR. This information could result in the development of resistant genotypes of soybean to <i>P. pachyrhizi</i>, and effective and specific products against the pathogen.https://www.mdpi.com/2218-1989/11/3/179Asian soybean rustsoybeanmetabolomicsUHPLC-MS/MSchemometricsGNPS |
| spellingShingle | Evandro Silva José Perez da Graça Carla Porto Rodolpho Martin do Prado Estela Nunes Francismar Corrêa Marcelino-Guimarães Mauricio Conrado Meyer Eduardo Jorge Pilau Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection Metabolites Asian soybean rust soybean metabolomics UHPLC-MS/MS chemometrics GNPS |
| title | Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection |
| title_full | Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection |
| title_fullStr | Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection |
| title_full_unstemmed | Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection |
| title_short | Untargeted Metabolomics Analysis by UHPLC-MS/MS of Soybean Plant in a Compatible Response to <i>Phakopsora pachyrhizi</i> Infection |
| title_sort | untargeted metabolomics analysis by uhplc ms ms of soybean plant in a compatible response to i phakopsora pachyrhizi i infection |
| topic | Asian soybean rust soybean metabolomics UHPLC-MS/MS chemometrics GNPS |
| url | https://www.mdpi.com/2218-1989/11/3/179 |
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