CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD
Background: Despite the availability of a large number of treatments for multiple sclerosis with various targets, these treatments are not always effective. According to the literature, experimental studies have shown a significant decrease in tumor necrosis factor alfa (TNF-α) with the use of ext...
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| Format: | Article |
| Language: | Russian |
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MONIKI
2016-07-01
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| Series: | Alʹmanah Kliničeskoj Mediciny |
| Subjects: | |
| Online Access: | https://www.almclinmed.ru/jour/article/view/354 |
| _version_ | 1818619073681948672 |
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| author | A. V. Kil'dyushevskiy S. V. Kotov O. P. Sidorova A. V. Borodin |
| author_facet | A. V. Kil'dyushevskiy S. V. Kotov O. P. Sidorova A. V. Borodin |
| author_sort | A. V. Kil'dyushevskiy |
| collection | DOAJ |
| description | Background: Despite the availability of a large number of treatments for multiple sclerosis with various targets, these treatments are not always effective. According to the literature, experimental studies have shown a significant decrease in tumor necrosis factor alfa (TNF-α) with the use of extracorporeal photochemotherapy. Aim: To assess changes in TNF-α in patients with multiple sclerosis during treatment with transimmunization. Materials and methods: The study recruited 13 adult patients with multiple sclerosis. Serum TNF-α was measured by immunochemiluminescence analysis (IMMULITE 1000, Siemens). The patients were treated by transimmunization, i.e. a modified photopheresis. Two hours before the procedure, Ammifurin (8-methoxypsoralene) was administered to all the patients, then their mononuclear cells were isolated under PBSC protocol with Haemonetics MCS+ cell separator. Thereafter, mononuclear cells were irradiated with ultraviolet for 90 minutes and incubated for 20 hours at 37 °С. The next day the cells were re-infused to the patients. The procedure was performed 2 times per week for 6 months, then once per 4 months. Results: Before transimmunization, mean TNF-α level in adult patients with multiple sclerosis was 9.958±0.812 pg/mL (normal, below 8.1 pg/mL). After transimmunization, its level was 6.992±0.367 pg/mL (р<0.05). Conclusion: Ultraviolet irradiation of peripheral blood monocytes with their subsequent incubation (transimmunization) led to a 30% decrease of serum TNF-α in patients with multiple sclerosis. This indicates a suppressive effect of transimmunization on TNF-α. Hence, in patients with multiple sclerosis transimmunization exerts an anti-inflammatory effect. |
| first_indexed | 2024-12-16T17:31:41Z |
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| id | doaj.art-177efdb966d8483c9762a05b861ccde4 |
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| issn | 2072-0505 2587-9294 |
| language | Russian |
| last_indexed | 2024-12-16T17:31:41Z |
| publishDate | 2016-07-01 |
| publisher | MONIKI |
| record_format | Article |
| series | Alʹmanah Kliničeskoj Mediciny |
| spelling | doaj.art-177efdb966d8483c9762a05b861ccde42022-12-21T22:22:54ZrusMONIKIAlʹmanah Kliničeskoj Mediciny2072-05052587-92942016-07-0144332432810.18786/2072-0505-2016-44-3-324-328353CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHODA. V. Kil'dyushevskiy0S. V. Kotov1O. P. Sidorova2A. V. Borodin3Moscow Regional Research and Clinical Institute (MONIKI)Moscow Regional Research and Clinical Institute (MONIKI)Moscow Regional Research and Clinical Institute (MONIKI)Moscow Regional Research and Clinical Institute (MONIKI)Background: Despite the availability of a large number of treatments for multiple sclerosis with various targets, these treatments are not always effective. According to the literature, experimental studies have shown a significant decrease in tumor necrosis factor alfa (TNF-α) with the use of extracorporeal photochemotherapy. Aim: To assess changes in TNF-α in patients with multiple sclerosis during treatment with transimmunization. Materials and methods: The study recruited 13 adult patients with multiple sclerosis. Serum TNF-α was measured by immunochemiluminescence analysis (IMMULITE 1000, Siemens). The patients were treated by transimmunization, i.e. a modified photopheresis. Two hours before the procedure, Ammifurin (8-methoxypsoralene) was administered to all the patients, then their mononuclear cells were isolated under PBSC protocol with Haemonetics MCS+ cell separator. Thereafter, mononuclear cells were irradiated with ultraviolet for 90 minutes and incubated for 20 hours at 37 °С. The next day the cells were re-infused to the patients. The procedure was performed 2 times per week for 6 months, then once per 4 months. Results: Before transimmunization, mean TNF-α level in adult patients with multiple sclerosis was 9.958±0.812 pg/mL (normal, below 8.1 pg/mL). After transimmunization, its level was 6.992±0.367 pg/mL (р<0.05). Conclusion: Ultraviolet irradiation of peripheral blood monocytes with their subsequent incubation (transimmunization) led to a 30% decrease of serum TNF-α in patients with multiple sclerosis. This indicates a suppressive effect of transimmunization on TNF-α. Hence, in patients with multiple sclerosis transimmunization exerts an anti-inflammatory effect.https://www.almclinmed.ru/jour/article/view/354multiple sclerosistransimmunizationphotopheresisextracorporeal photochemotherapy |
| spellingShingle | A. V. Kil'dyushevskiy S. V. Kotov O. P. Sidorova A. V. Borodin CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD Alʹmanah Kliničeskoj Mediciny multiple sclerosis transimmunization photopheresis extracorporeal photochemotherapy |
| title | CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD |
| title_full | CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD |
| title_fullStr | CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD |
| title_full_unstemmed | CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD |
| title_short | CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD |
| title_sort | changes in tumor necrosis factor alfa during treatment of patients with multiple sclerosis by transimmunization method |
| topic | multiple sclerosis transimmunization photopheresis extracorporeal photochemotherapy |
| url | https://www.almclinmed.ru/jour/article/view/354 |
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