The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning
Plant-derived monoterpenoids have been shown to possess various biological effects, providing a scientific basis for their potential usage as antibacterial agents. Therefore, considering problems surrounding bacteria′s antibacterial resistance, the utilization of natural antimicrobial compounds such...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/19/3405 |
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| author | Tahmineh Hemmatian Kwon Ho Seo Meltem Yanilmaz Juran Kim |
| author_facet | Tahmineh Hemmatian Kwon Ho Seo Meltem Yanilmaz Juran Kim |
| author_sort | Tahmineh Hemmatian |
| collection | DOAJ |
| description | Plant-derived monoterpenoids have been shown to possess various biological effects, providing a scientific basis for their potential usage as antibacterial agents. Therefore, considering problems surrounding bacteria′s antibacterial resistance, the utilization of natural antimicrobial compounds such as monoterpenoids in different industries has gained much attention. The aim of this study was to fabricate and characterize various concentrations of plant-derived monoterpenoids, geraniol (G) and carvacrol (C), loaded into poly(lactic acid) (PLA) nanofibers via emulsion electrospinning. The antibacterial activities of the fabricated nanofibers were evaluated using three types of antibacterial assays (inhibition zone tests, live/dead bacterial cell assays, and antibacterial kinetic growth assays). Among the samples, 10 wt% carvacrol-loaded PLA nanofibers (C10) had the most bactericidal activity, with the widest inhibition zone of 5.26 cm and the highest visible dead bacteria using the inhibition zone test and live/dead bacterial cell assay. In order to quantitatively analyze the antibacterial activities of 5 wt% carvacrol-loaded PLA nanofibers (C5), C10, 5 wt% geraniol-loaded PLA nanofibers (G5), and 10 wt% geraniol-loaded PLA nanofibers (G10) against <i>E. coli</i> and <i>S.</i><i>epidermidis</i>, growth kinetic curves were analyzed using OD<sub>600</sub>. For the results, we found that the antibacterial performance was as follows: C10 > C5 > G10 > G5. Overall, carvacrol or geraniol-loaded PLA nanofibers are promising antibacterial materials for improving fiber functionality. |
| first_indexed | 2024-03-10T06:52:45Z |
| format | Article |
| id | doaj.art-17821178ffec452485f0de2323705d09 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T06:52:45Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-17821178ffec452485f0de2323705d092023-11-22T16:40:10ZengMDPI AGPolymers2073-43602021-10-011319340510.3390/polym13193405The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion ElectrospinningTahmineh Hemmatian0Kwon Ho Seo1Meltem Yanilmaz2Juran Kim3Advanced Textile R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaAdvanced Textile R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaTextile Engineering, Istanbul Technical University, Istanbul 34467, TurkeyAdvanced Textile R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, KoreaPlant-derived monoterpenoids have been shown to possess various biological effects, providing a scientific basis for their potential usage as antibacterial agents. Therefore, considering problems surrounding bacteria′s antibacterial resistance, the utilization of natural antimicrobial compounds such as monoterpenoids in different industries has gained much attention. The aim of this study was to fabricate and characterize various concentrations of plant-derived monoterpenoids, geraniol (G) and carvacrol (C), loaded into poly(lactic acid) (PLA) nanofibers via emulsion electrospinning. The antibacterial activities of the fabricated nanofibers were evaluated using three types of antibacterial assays (inhibition zone tests, live/dead bacterial cell assays, and antibacterial kinetic growth assays). Among the samples, 10 wt% carvacrol-loaded PLA nanofibers (C10) had the most bactericidal activity, with the widest inhibition zone of 5.26 cm and the highest visible dead bacteria using the inhibition zone test and live/dead bacterial cell assay. In order to quantitatively analyze the antibacterial activities of 5 wt% carvacrol-loaded PLA nanofibers (C5), C10, 5 wt% geraniol-loaded PLA nanofibers (G5), and 10 wt% geraniol-loaded PLA nanofibers (G10) against <i>E. coli</i> and <i>S.</i><i>epidermidis</i>, growth kinetic curves were analyzed using OD<sub>600</sub>. For the results, we found that the antibacterial performance was as follows: C10 > C5 > G10 > G5. Overall, carvacrol or geraniol-loaded PLA nanofibers are promising antibacterial materials for improving fiber functionality.https://www.mdpi.com/2073-4360/13/19/3405monoterpenoidcarvacrolgeraniol<i>Escherichia coli</i><i>Staphylococcus epidermidis</i>poly (lactic acid) |
| spellingShingle | Tahmineh Hemmatian Kwon Ho Seo Meltem Yanilmaz Juran Kim The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning Polymers monoterpenoid carvacrol geraniol <i>Escherichia coli</i> <i>Staphylococcus epidermidis</i> poly (lactic acid) |
| title | The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning |
| title_full | The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning |
| title_fullStr | The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning |
| title_full_unstemmed | The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning |
| title_short | The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning |
| title_sort | bacterial control of poly lactic acid nanofibers loaded with plant derived monoterpenoids via emulsion electrospinning |
| topic | monoterpenoid carvacrol geraniol <i>Escherichia coli</i> <i>Staphylococcus epidermidis</i> poly (lactic acid) |
| url | https://www.mdpi.com/2073-4360/13/19/3405 |
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