Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats
Abstract Naja nigricollis Venom (NnV) contains complex toxins that affects various vital systems functions after envenoming. The venom toxins have been reported to induce male reproductive disorders in envenomed rats. This present study explored the ameliorative potential of kaempferol on NnV-induce...
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| Format: | Article |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54523-w |
| _version_ | 1797275122619383808 |
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| author | Akindele Oluwatosin Adeyi Babafemi Siji Ajisebiola Akanni Abubakar Sanni Johnson Olaleye Oladele Abdur-Rahman Kolawole Mustapha Omotayo Opemipo Oyedara Olukunle Silas Fagbenro |
| author_facet | Akindele Oluwatosin Adeyi Babafemi Siji Ajisebiola Akanni Abubakar Sanni Johnson Olaleye Oladele Abdur-Rahman Kolawole Mustapha Omotayo Opemipo Oyedara Olukunle Silas Fagbenro |
| author_sort | Akindele Oluwatosin Adeyi |
| collection | DOAJ |
| description | Abstract Naja nigricollis Venom (NnV) contains complex toxins that affects various vital systems functions after envenoming. The venom toxins have been reported to induce male reproductive disorders in envenomed rats. This present study explored the ameliorative potential of kaempferol on NnV-induced male reproductive toxicity. Fifty male wistar rats were sorted randomly into five groups (n = 10) for this study. Group 1 were noted as the control, while rats in groups 2 to 5 were injected with LD50 of NnV (1.0 mg/kg bw; i.p.). Group 2 was left untreated post envenomation while group 3 was treated with 0.2 ml of polyvalent antivenom. Groups 4 and 5 were treated with 4 and 8 mg/kg of kaempferol, respectively. NnV caused substantial reduction in concentrations of follicle stimulating hormone, testosterone and luteinizing hormone, while sperm motility, volume and counts significantly (p < 0.05) decreased in envenomed untreated rats. The venom enhanced malondialdehyde levels and substantially decreased glutathione levels, superoxide dismutase and glutathione peroxidase activities in the testes and epididymis of envenomed untreated rats. Additionally, epididymal and testicular myeloperoxidase activity and nitric oxide levels were elevated which substantiated severe morphological defects noticed in the reproductive organs. However, treatment of envenomed rats with kaempferol normalized the reproductive hormones with significant improvement on sperm functional parameters. Elevated inflammatory and oxidative stress biomarkers in testis and epididymis were suppressed post kaempferol treatment. Severe histopathological lesions in the epididymal and testicular tissues were ameliorated in the envenomed treated groups. Results highlights the significance of kaempferol in mitigating reproductive toxicity induced after snakebite envenoming. |
| first_indexed | 2024-03-07T15:09:49Z |
| format | Article |
| id | doaj.art-1783b00b269e4472a1b9a036632dac33 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:09:49Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1783b00b269e4472a1b9a036632dac332024-03-05T18:43:08ZengNature PortfolioScientific Reports2045-23222024-02-0114111310.1038/s41598-024-54523-wKaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male ratsAkindele Oluwatosin Adeyi0Babafemi Siji Ajisebiola1Akanni Abubakar Sanni2Johnson Olaleye Oladele3Abdur-Rahman Kolawole Mustapha4Omotayo Opemipo Oyedara5Olukunle Silas Fagbenro6Animal Physiology Unit, Department of Zoology, University of IbadanDepartment of Zoology, Osun State UniversityDepartment of Zoology, Osun State UniversityBiochemistry Unit, Department of Chemical Sciences, Kings UniversityDepartment of Zoology, Osun State UniversityDepartment of Biotechnology, Osun State UniversityCell Biology and Genetics Unit, Department of Zoology, University of IbadanAbstract Naja nigricollis Venom (NnV) contains complex toxins that affects various vital systems functions after envenoming. The venom toxins have been reported to induce male reproductive disorders in envenomed rats. This present study explored the ameliorative potential of kaempferol on NnV-induced male reproductive toxicity. Fifty male wistar rats were sorted randomly into five groups (n = 10) for this study. Group 1 were noted as the control, while rats in groups 2 to 5 were injected with LD50 of NnV (1.0 mg/kg bw; i.p.). Group 2 was left untreated post envenomation while group 3 was treated with 0.2 ml of polyvalent antivenom. Groups 4 and 5 were treated with 4 and 8 mg/kg of kaempferol, respectively. NnV caused substantial reduction in concentrations of follicle stimulating hormone, testosterone and luteinizing hormone, while sperm motility, volume and counts significantly (p < 0.05) decreased in envenomed untreated rats. The venom enhanced malondialdehyde levels and substantially decreased glutathione levels, superoxide dismutase and glutathione peroxidase activities in the testes and epididymis of envenomed untreated rats. Additionally, epididymal and testicular myeloperoxidase activity and nitric oxide levels were elevated which substantiated severe morphological defects noticed in the reproductive organs. However, treatment of envenomed rats with kaempferol normalized the reproductive hormones with significant improvement on sperm functional parameters. Elevated inflammatory and oxidative stress biomarkers in testis and epididymis were suppressed post kaempferol treatment. Severe histopathological lesions in the epididymal and testicular tissues were ameliorated in the envenomed treated groups. Results highlights the significance of kaempferol in mitigating reproductive toxicity induced after snakebite envenoming.https://doi.org/10.1038/s41598-024-54523-w |
| spellingShingle | Akindele Oluwatosin Adeyi Babafemi Siji Ajisebiola Akanni Abubakar Sanni Johnson Olaleye Oladele Abdur-Rahman Kolawole Mustapha Omotayo Opemipo Oyedara Olukunle Silas Fagbenro Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats Scientific Reports |
| title | Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats |
| title_full | Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats |
| title_fullStr | Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats |
| title_full_unstemmed | Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats |
| title_short | Kaempferol mitigates reproductive dysfunctions induced by Naja nigricollis venom through antioxidant system and anti-inflammatory response in male rats |
| title_sort | kaempferol mitigates reproductive dysfunctions induced by naja nigricollis venom through antioxidant system and anti inflammatory response in male rats |
| url | https://doi.org/10.1038/s41598-024-54523-w |
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