Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica
The present study included the microscopic and molecular identification of Entamoeba histolytica by using specific primers to detect four virulence factors possessed by Entamoeba histolytica. Virulence factors included Active Cysteine proteinase, Galactose/N-acetyl-D-galactose-lectin, Amoeba pore C...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2024-03-01
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| Series: | Baghdad Science Journal |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7205 |
| _version_ | 1797289085482565632 |
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| author | Lima Tariq Youash Lazar Shihab Ahmed Al-Juboury Mohammad Nadhir Maaroof |
| author_facet | Lima Tariq Youash Lazar Shihab Ahmed Al-Juboury Mohammad Nadhir Maaroof |
| author_sort | Lima Tariq Youash Lazar |
| collection | DOAJ |
| description |
The present study included the microscopic and molecular identification of Entamoeba histolytica by using specific primers to detect four virulence factors possessed by Entamoeba histolytica. Virulence factors included Active Cysteine proteinase, Galactose/N-acetyl-D-galactose-lectin, Amoeba pore C and Phospholipase. Titanium dioxide nanoparticles (TiO2NPs) were synthesized from Pseudomonas aeruginosa which producing Pyocyanin pigment as a reducing agent to form it. After that we studied the ability ofTiO2NPs to inhibit virulence factors production and curing the genes responsible for encoding them by using four different dose 2 ,3, 4, 6 mg/Kg and administered by intraperitoneal injection to laboratory mice Mus musculus. The infection was molecularly confirmed, then the treatment for a period of ten days, as it was given on the third, fifth, seventh, and tenth days. The results of the study showed the inability of dose 2, 3 mg/Kg of TiO2NPs to curing the gene responsible for active cysteine proteinase and Gal/Gal NAC lectin upon treatment on the third to the tenth day, but was able to curing the genes when using dose (4,6) mg/Kg, Statistical analysis was performed , it was found that there were significant differences between the use of different dose and days of treatment. While Amoeba pore C, it was found that the four dose did not affect the inhibition on the third day of treatment. While dose 4,6 mg/Kg were able to curing the gene responsible for its encoding. Statistical analysis showed significant differences between the use of TiO2NPs and the days of treatment. Finally, the results showed that the four doses do not affect on phospholipase. Once, performing the statistical analysis it was found that there were no significant differences.
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| first_indexed | 2024-03-07T18:59:06Z |
| format | Article |
| id | doaj.art-17868ef3a61845a0b4c91ab19d8da2e9 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-03-07T18:59:06Z |
| publishDate | 2024-03-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-17868ef3a61845a0b4c91ab19d8da2e92024-03-01T20:06:37ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862024-03-0121310.21123/bsj.2023.7205Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolyticaLima Tariq Youash Lazar0Shihab Ahmed Al-Juboury1Mohammad Nadhir Maaroof2Department of Basic Nursing Sciences, College of Nursing, University of Kirkuk, Kirkuk, Iraq.Department of Biology, College of Education for pure sciences, University of Tikrit, Tikrit, Iraq.College of Medicine, University of Tikrit, Tikrit, Iraq. The present study included the microscopic and molecular identification of Entamoeba histolytica by using specific primers to detect four virulence factors possessed by Entamoeba histolytica. Virulence factors included Active Cysteine proteinase, Galactose/N-acetyl-D-galactose-lectin, Amoeba pore C and Phospholipase. Titanium dioxide nanoparticles (TiO2NPs) were synthesized from Pseudomonas aeruginosa which producing Pyocyanin pigment as a reducing agent to form it. After that we studied the ability ofTiO2NPs to inhibit virulence factors production and curing the genes responsible for encoding them by using four different dose 2 ,3, 4, 6 mg/Kg and administered by intraperitoneal injection to laboratory mice Mus musculus. The infection was molecularly confirmed, then the treatment for a period of ten days, as it was given on the third, fifth, seventh, and tenth days. The results of the study showed the inability of dose 2, 3 mg/Kg of TiO2NPs to curing the gene responsible for active cysteine proteinase and Gal/Gal NAC lectin upon treatment on the third to the tenth day, but was able to curing the genes when using dose (4,6) mg/Kg, Statistical analysis was performed , it was found that there were significant differences between the use of different dose and days of treatment. While Amoeba pore C, it was found that the four dose did not affect the inhibition on the third day of treatment. While dose 4,6 mg/Kg were able to curing the gene responsible for its encoding. Statistical analysis showed significant differences between the use of TiO2NPs and the days of treatment. Finally, the results showed that the four doses do not affect on phospholipase. Once, performing the statistical analysis it was found that there were no significant differences. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7205Curing genes, Entamoeba histolytica, Pyocyanin pigment, Pseudomonas aeruginosa, TiO2NPs, Virulence factors |
| spellingShingle | Lima Tariq Youash Lazar Shihab Ahmed Al-Juboury Mohammad Nadhir Maaroof Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica Baghdad Science Journal Curing genes, Entamoeba histolytica, Pyocyanin pigment, Pseudomonas aeruginosa, TiO2NPs, Virulence factors |
| title | Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica |
| title_full | Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica |
| title_fullStr | Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica |
| title_full_unstemmed | Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica |
| title_short | Molecular detection of the ability of Biosynthesized Titanium dioxide nanoparticles to curing some genes of virulence factors of Entamoeba histolytica |
| title_sort | molecular detection of the ability of biosynthesized titanium dioxide nanoparticles to curing some genes of virulence factors of entamoeba histolytica |
| topic | Curing genes, Entamoeba histolytica, Pyocyanin pigment, Pseudomonas aeruginosa, TiO2NPs, Virulence factors |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7205 |
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