First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques
Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and fae...
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MDPI AG
2022-11-01
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| Series: | Animals |
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| author | Sanaa M. Idris Eva A. Ali Wisal A. Elmagzoub Julius B. Okuni Mohamed E. Mukhtar Lonzy Ojok ElSagad Eltayeb Ahmed Abd El Wahed Kamal H. Eltom Ahmed A. Gameel |
| author_facet | Sanaa M. Idris Eva A. Ali Wisal A. Elmagzoub Julius B. Okuni Mohamed E. Mukhtar Lonzy Ojok ElSagad Eltayeb Ahmed Abd El Wahed Kamal H. Eltom Ahmed A. Gameel |
| author_sort | Sanaa M. Idris |
| collection | DOAJ |
| description | Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and faecal samples were collected from all animals (N = 59): harvested sera were tested for anti-<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) antibodies by Enzyme Linked Immunosorbent Assay (ELISA); faeces were screened for acid-fast bacilli by Ziehl–Neelsen staining, tested for MAP DNA by recombinase polymerase amplification (RPA) and some faecal samples were cultured for MAP isolation. Typical MAP acid-fast bacilli were seen in 10.2% (6/59) of the faecal smears, 37.5% of the tested faecal samples (12/32) were positive for MAP DNA and only 3 (5.1%) animals were seropositive for MAP. MAP positive cultures were obtained from 2 out the 6 samples showing typical MAP acid-fast bacilli; the isolates were confirmed by real-time PCR and sequencing. As sheep are animals of utmost economic importance as the main export animals for the country, this first report of ovine PTB warrants special considerations and more investigations for planning control programmes of the disease. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T17:54:56Z |
| publishDate | 2022-11-01 |
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| series | Animals |
| spelling | doaj.art-178b09b6100e4ddf980ed5920a9eec7b2023-11-24T10:25:02ZengMDPI AGAnimals2076-26152022-11-011223331210.3390/ani12233312First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different TechniquesSanaa M. Idris0Eva A. Ali1Wisal A. Elmagzoub2Julius B. Okuni3Mohamed E. Mukhtar4Lonzy Ojok5ElSagad Eltayeb6Ahmed Abd El Wahed7Kamal H. Eltom8Ahmed A. Gameel9Department of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, SudanDepartment of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, SudanDepartment of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, SudanCollege of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, Kampala P.O. Box 7062, UgandaDepartment of Agricultural Extension and Rural Development, Faculty of Agriculture, University of Khartoum, Shambat 13314, Khartoum North, SudanCollege of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, Kampala P.O. Box 7062, UgandaDepartment of Surgery, Faculty of Medicine, Al Neelain University, Almogran 11111, Khartoum, SudanInstitute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 43, D-04103 Leipzig, GermanyDepartment of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, SudanDepartment of Pathology, Faculty of Veterinary Medicine, University of Khartoum, Shambat 13314, Khartoum North, SudanParatuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and faecal samples were collected from all animals (N = 59): harvested sera were tested for anti-<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) antibodies by Enzyme Linked Immunosorbent Assay (ELISA); faeces were screened for acid-fast bacilli by Ziehl–Neelsen staining, tested for MAP DNA by recombinase polymerase amplification (RPA) and some faecal samples were cultured for MAP isolation. Typical MAP acid-fast bacilli were seen in 10.2% (6/59) of the faecal smears, 37.5% of the tested faecal samples (12/32) were positive for MAP DNA and only 3 (5.1%) animals were seropositive for MAP. MAP positive cultures were obtained from 2 out the 6 samples showing typical MAP acid-fast bacilli; the isolates were confirmed by real-time PCR and sequencing. As sheep are animals of utmost economic importance as the main export animals for the country, this first report of ovine PTB warrants special considerations and more investigations for planning control programmes of the disease.https://www.mdpi.com/2076-2615/12/23/3312<i>Mycobacterium avium paratuberculosis</i>sheeprecombinase polymerase amplification |
| spellingShingle | Sanaa M. Idris Eva A. Ali Wisal A. Elmagzoub Julius B. Okuni Mohamed E. Mukhtar Lonzy Ojok ElSagad Eltayeb Ahmed Abd El Wahed Kamal H. Eltom Ahmed A. Gameel First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques Animals <i>Mycobacterium avium paratuberculosis</i> sheep recombinase polymerase amplification |
| title | First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques |
| title_full | First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques |
| title_fullStr | First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques |
| title_full_unstemmed | First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques |
| title_short | First Report on Ovine Paratuberculosis in the Sudan: Diagnosis Using Different Techniques |
| title_sort | first report on ovine paratuberculosis in the sudan diagnosis using different techniques |
| topic | <i>Mycobacterium avium paratuberculosis</i> sheep recombinase polymerase amplification |
| url | https://www.mdpi.com/2076-2615/12/23/3312 |
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