Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms
An improved UV spectrophotometric method has been developed and validated for precise, efficient and selective determination of dexamethasone in tablets and capsules. The quantitative analyses were carried out using ethanol/water (2:1 v/v) as background electrolyte and UV detection was carried out a...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2015-03-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | https://periodicos.ufms.br/index.php/orbital/article/view/17895 |
| _version_ | 1797946754430140416 |
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| author | Rúbia Adrieli Sversut James Cabral Vieira Aline Marques Rosa Anil Kumar Singh Marcos Serrou do Amaral Nájla Mohamad Kassab |
| author_facet | Rúbia Adrieli Sversut James Cabral Vieira Aline Marques Rosa Anil Kumar Singh Marcos Serrou do Amaral Nájla Mohamad Kassab |
| author_sort | Rúbia Adrieli Sversut |
| collection | DOAJ |
| description | An improved UV spectrophotometric method has been developed and validated for precise, efficient and selective determination of dexamethasone in tablets and capsules. The quantitative analyses were carried out using ethanol/water (2:1 v/v) as background electrolyte and UV detection was carried out at 240 nm. The calibration curve was linear over a concentration range from 4.0 to 40.0 µg mL-1. The average recovery was 97.60 ± 1.06% for tablets and 96.64 ±0.87% for capsules. The limit of detection and limit of quantification were 0.63 and 1.90 µg mL-1, respectively. The method was validated according to ICH guidelines and the acceptance criteria for accuracy, precision, linearity, specificity and system suitability were met. The results obtained with proposed method confirm improved performance over other methods found in the literature.
DOI: http://dx.doi.org/10.17807/orbital.v7i1.630
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| first_indexed | 2024-04-10T21:16:03Z |
| format | Article |
| id | doaj.art-178c5827e90f463c8f8938ea0f521967 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-04-10T21:16:03Z |
| publishDate | 2015-03-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-178c5827e90f463c8f8938ea0f5219672023-01-20T11:05:45ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282015-03-0171Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage FormsRúbia Adrieli Sversut0James Cabral Vieira1Aline Marques Rosa2Anil Kumar Singh3Marcos Serrou do Amaral4Nájla Mohamad Kassab5Universidade Federal de Mato Grosso do SulUniversidade Federal de Mato Grosso do SulUniversidade Federal de Mato Grosso do SulUniversidade de São Paulo, Faculdade de Ciências FarmacêuticasUniversidade Federal de Mato Grosso do SulUniversidade Federal de Mato Grosso do SulAn improved UV spectrophotometric method has been developed and validated for precise, efficient and selective determination of dexamethasone in tablets and capsules. The quantitative analyses were carried out using ethanol/water (2:1 v/v) as background electrolyte and UV detection was carried out at 240 nm. The calibration curve was linear over a concentration range from 4.0 to 40.0 µg mL-1. The average recovery was 97.60 ± 1.06% for tablets and 96.64 ±0.87% for capsules. The limit of detection and limit of quantification were 0.63 and 1.90 µg mL-1, respectively. The method was validated according to ICH guidelines and the acceptance criteria for accuracy, precision, linearity, specificity and system suitability were met. The results obtained with proposed method confirm improved performance over other methods found in the literature. DOI: http://dx.doi.org/10.17807/orbital.v7i1.630 https://periodicos.ufms.br/index.php/orbital/article/view/17895glucocorticoidquantificationdrugpharmaceutical preparation |
| spellingShingle | Rúbia Adrieli Sversut James Cabral Vieira Aline Marques Rosa Anil Kumar Singh Marcos Serrou do Amaral Nájla Mohamad Kassab Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms Orbital: The Electronic Journal of Chemistry glucocorticoid quantification drug pharmaceutical preparation |
| title | Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms |
| title_full | Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms |
| title_fullStr | Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms |
| title_full_unstemmed | Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms |
| title_short | Improved UV Spectrophotometric Method for Precise, Efficient and Selective Determination of Dexamethasone in Pharmaceutical Dosage Forms |
| title_sort | improved uv spectrophotometric method for precise efficient and selective determination of dexamethasone in pharmaceutical dosage forms |
| topic | glucocorticoid quantification drug pharmaceutical preparation |
| url | https://periodicos.ufms.br/index.php/orbital/article/view/17895 |
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