Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering
In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into m...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-12-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731417744157 |
| _version_ | 1818967868767731712 |
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| author | Lukas Kessler Sandra Gehrke Marc Winnefeld Birgit Huber Eva Hoch Torsten Walter Ralf Wyrwa Matthias Schnabelrauch Malte Schmidt Maximilian Kückelhaus Marcus Lehnhardt Tobias Hirsch Frank Jacobsen |
| author_facet | Lukas Kessler Sandra Gehrke Marc Winnefeld Birgit Huber Eva Hoch Torsten Walter Ralf Wyrwa Matthias Schnabelrauch Malte Schmidt Maximilian Kückelhaus Marcus Lehnhardt Tobias Hirsch Frank Jacobsen |
| author_sort | Lukas Kessler |
| collection | DOAJ |
| description | In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs. |
| first_indexed | 2024-12-20T13:55:38Z |
| format | Article |
| id | doaj.art-178c8544b5ee40f3ba8ebe1f5fc6d950 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-20T13:55:38Z |
| publishDate | 2017-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-178c8544b5ee40f3ba8ebe1f5fc6d9502022-12-21T19:38:27ZengSAGE PublishingJournal of Tissue Engineering2041-73142017-12-01810.1177/2041731417744157Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineeringLukas Kessler0Sandra Gehrke1Marc Winnefeld2Birgit Huber3Eva Hoch4Torsten Walter5Ralf Wyrwa6Matthias Schnabelrauch7Malte Schmidt8Maximilian Kückelhaus9Marcus Lehnhardt10Tobias Hirsch11Frank Jacobsen12Department of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyResearch and Development, Beiersdorf AG, Hamburg, GermanyResearch and Development, Beiersdorf AG, Hamburg, GermanyInstitute for Interfacial Engineering and Plasma Technology, University of Stuttgart, Stuttgart, GermanyInstitute for Interfacial Engineering and Plasma Technology, University of Stuttgart, Stuttgart, GermanyBiomaterials Department, INNOVENT e. V., Jena, GermanyBiomaterials Department, INNOVENT e. V., Jena, GermanyBiomaterials Department, INNOVENT e. V., Jena, GermanyDepartment of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyDepartment of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyDepartment of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyDepartment of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyDepartment of Plastic Surgery and Burn Centre, BG University Hospital Bergmannsheil GmbH, Ruhr University Bochum, Bochum, GermanyIn vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.https://doi.org/10.1177/2041731417744157 |
| spellingShingle | Lukas Kessler Sandra Gehrke Marc Winnefeld Birgit Huber Eva Hoch Torsten Walter Ralf Wyrwa Matthias Schnabelrauch Malte Schmidt Maximilian Kückelhaus Marcus Lehnhardt Tobias Hirsch Frank Jacobsen Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering Journal of Tissue Engineering |
| title | Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering |
| title_full | Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering |
| title_fullStr | Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering |
| title_full_unstemmed | Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering |
| title_short | Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering |
| title_sort | methacrylated gelatin hyaluronan based hydrogels for soft tissue engineering |
| url | https://doi.org/10.1177/2041731417744157 |
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