Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation
We present a double emulsion drop-based microfluidic approach to produce uniform polyacrylic acid functionalized polyethylene glycol (PAA-PEG) microgels. By utilizing double emulsion drops as templates, we produce monodisperse microgels by rapid photopolymerization of the inner prepolymer drop consi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Sensors |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fsens.2022.1016791/full |
| _version_ | 1811205025607712768 |
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| author | Yoon Choi Su-Ryeon Park Sei-Jung Lee Chang-Hyung Choi |
| author_facet | Yoon Choi Su-Ryeon Park Sei-Jung Lee Chang-Hyung Choi |
| author_sort | Yoon Choi |
| collection | DOAJ |
| description | We present a double emulsion drop-based microfluidic approach to produce uniform polyacrylic acid functionalized polyethylene glycol (PAA-PEG) microgels. By utilizing double emulsion drops as templates, we produce monodisperse microgels by rapid photopolymerization of the inner prepolymer drop consisting of polyacrylic acid (PAA) and polyethylene glycol diacrylate (PEGDA), followed by dewetting the oil layer when they disperse into an aqueous media. The size control of the PAA-PEG microgels with a broad range is achieved by tuning the flow rate of each phase; the uniformity of the microgels is maintained even when the flow rate changes. The results show rapid R-phycoerythrin (R-PE) coupling with the microgels’ carboxylate with minimal non-specific adsorption, demonstrating highly efficient and reliable biomolecular conjugation within PAA-PEG microgels. |
| first_indexed | 2024-04-12T03:24:43Z |
| format | Article |
| id | doaj.art-178d29e14bcc4a47aea26fbebf91a30a |
| institution | Directory Open Access Journal |
| issn | 2673-5067 |
| language | English |
| last_indexed | 2024-04-12T03:24:43Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sensors |
| spelling | doaj.art-178d29e14bcc4a47aea26fbebf91a30a2022-12-22T03:49:46ZengFrontiers Media S.A.Frontiers in Sensors2673-50672022-10-01310.3389/fsens.2022.10167911016791Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugationYoon Choi0Su-Ryeon Park1Sei-Jung Lee2Chang-Hyung Choi3Division of Cosmetic Science and Technology, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, South KoreaDivision of Cosmetic Science and Technology, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, South KoreaDepartment of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, South KoreaDivision of Cosmetic Science and Technology, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, South KoreaWe present a double emulsion drop-based microfluidic approach to produce uniform polyacrylic acid functionalized polyethylene glycol (PAA-PEG) microgels. By utilizing double emulsion drops as templates, we produce monodisperse microgels by rapid photopolymerization of the inner prepolymer drop consisting of polyacrylic acid (PAA) and polyethylene glycol diacrylate (PEGDA), followed by dewetting the oil layer when they disperse into an aqueous media. The size control of the PAA-PEG microgels with a broad range is achieved by tuning the flow rate of each phase; the uniformity of the microgels is maintained even when the flow rate changes. The results show rapid R-phycoerythrin (R-PE) coupling with the microgels’ carboxylate with minimal non-specific adsorption, demonstrating highly efficient and reliable biomolecular conjugation within PAA-PEG microgels.https://www.frontiersin.org/articles/10.3389/fsens.2022.1016791/fullmicrogelmicrofluidicsbiomolecular conjugationpolyacrylic acidhydrogel |
| spellingShingle | Yoon Choi Su-Ryeon Park Sei-Jung Lee Chang-Hyung Choi Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation Frontiers in Sensors microgel microfluidics biomolecular conjugation polyacrylic acid hydrogel |
| title | Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation |
| title_full | Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation |
| title_fullStr | Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation |
| title_full_unstemmed | Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation |
| title_short | Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation |
| title_sort | microfluidic production of polyacrylic acid functionalized peg microgels for efficient biomolecular conjugation |
| topic | microgel microfluidics biomolecular conjugation polyacrylic acid hydrogel |
| url | https://www.frontiersin.org/articles/10.3389/fsens.2022.1016791/full |
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