Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation
CircRNA is a type of closed circular non-coding RNA formed by reverse splicing and plays an important role in regulating the growth and development of plants and animals. To investigate the function of circ-FoxO3 in mouse myoblast cells’ (C2C12) differentiation and proliferation, we used R...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/6/616 |
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| author | Xiaoyue Li Cunyuan Li Zhijin Liu Wei Ni Rui Yao Yueren Xu Renzhe Quan Mengdan Zhang Huixiang Li Li Liu Shengwei Hu |
| author_facet | Xiaoyue Li Cunyuan Li Zhijin Liu Wei Ni Rui Yao Yueren Xu Renzhe Quan Mengdan Zhang Huixiang Li Li Liu Shengwei Hu |
| author_sort | Xiaoyue Li |
| collection | DOAJ |
| description | CircRNA is a type of closed circular non-coding RNA formed by reverse splicing and plays an important role in regulating the growth and development of plants and animals. To investigate the function of circ-FoxO3 in mouse myoblast cells’ (C2C12) differentiation and proliferation, we used RT-qPCR to detect the expression level of circ-FoxO3 in mouse myoblast cells at different densities and different differentiation stages, and the specific interference fragment was used to inhibit the expression level of circ-FoxO3 in myoblast cells to observe its effect on myoblast cells proliferation and differentiation. We found that the expression level of circ-FoxO3 in myoblast cells increased with the prolongation of myoblast cells differentiation time, and its expression level decreased with the proliferation of myoblast cells. At the same time, we found that the differentiation ability of the cells was significantly increased (<i>p</i> < 0.05), but the cell proliferation was unchanged (<i>p</i> > 0.05) after inhibiting the expression of circ-FoxO3 in myoblast cells. Combining the results of bioinformatics analysis and the dual luciferase reporter experiment, we found that circ-FoxO3 is a sponge of miR-138-5p, which regulates muscle differentiation. Our study shows that circ-FoxO3 can inhibit the differentiation of C2C12 myoblast cells and lay a scientific foundation for further study of skeletal muscle development at circRNA levels. |
| first_indexed | 2024-03-12T05:54:09Z |
| format | Article |
| id | doaj.art-178d3cc3382a41e69e9fb9bab903919d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T05:54:09Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-178d3cc3382a41e69e9fb9bab903919d2023-09-03T04:48:17ZengMDPI AGCells2073-44092019-06-018661610.3390/cells8060616cells8060616Circular RNA circ-FoxO3 Inhibits Myoblast Cells DifferentiationXiaoyue Li0Cunyuan Li1Zhijin Liu2Wei Ni3Rui Yao4Yueren Xu5Renzhe Quan6Mengdan Zhang7Huixiang Li8Li Liu9Shengwei Hu10College of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCollege of Life Sciences, Shihezi University, Xinjiang 832003, ChinaCircRNA is a type of closed circular non-coding RNA formed by reverse splicing and plays an important role in regulating the growth and development of plants and animals. To investigate the function of circ-FoxO3 in mouse myoblast cells’ (C2C12) differentiation and proliferation, we used RT-qPCR to detect the expression level of circ-FoxO3 in mouse myoblast cells at different densities and different differentiation stages, and the specific interference fragment was used to inhibit the expression level of circ-FoxO3 in myoblast cells to observe its effect on myoblast cells proliferation and differentiation. We found that the expression level of circ-FoxO3 in myoblast cells increased with the prolongation of myoblast cells differentiation time, and its expression level decreased with the proliferation of myoblast cells. At the same time, we found that the differentiation ability of the cells was significantly increased (<i>p</i> < 0.05), but the cell proliferation was unchanged (<i>p</i> > 0.05) after inhibiting the expression of circ-FoxO3 in myoblast cells. Combining the results of bioinformatics analysis and the dual luciferase reporter experiment, we found that circ-FoxO3 is a sponge of miR-138-5p, which regulates muscle differentiation. Our study shows that circ-FoxO3 can inhibit the differentiation of C2C12 myoblast cells and lay a scientific foundation for further study of skeletal muscle development at circRNA levels.https://www.mdpi.com/2073-4409/8/6/616circ-FoxO3C2C12 myoblast cellscell proliferationcell differentiationmiRNA sponge |
| spellingShingle | Xiaoyue Li Cunyuan Li Zhijin Liu Wei Ni Rui Yao Yueren Xu Renzhe Quan Mengdan Zhang Huixiang Li Li Liu Shengwei Hu Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation Cells circ-FoxO3 C2C12 myoblast cells cell proliferation cell differentiation miRNA sponge |
| title | Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation |
| title_full | Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation |
| title_fullStr | Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation |
| title_full_unstemmed | Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation |
| title_short | Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation |
| title_sort | circular rna circ foxo3 inhibits myoblast cells differentiation |
| topic | circ-FoxO3 C2C12 myoblast cells cell proliferation cell differentiation miRNA sponge |
| url | https://www.mdpi.com/2073-4409/8/6/616 |
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