A study of Lumpy skin disease outbreak in Thi Qar Province
In this study the clinical diagnosis of lumpy skin disease was depended. A total 1606 serum samples and skin biopsies collected from infected cows clinically with skin nodule of different size which change to a necrotic nodule or form a deep scab, from wide different areas and cities of Thi Qar , ex...
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Format: | Article |
Language: | English |
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University of Thi-Qar
2019-04-01
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Series: | مجلة علوم ذي قار |
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Online Access: | https://mail.jsci.utq.edu.iq/index.php/main/article/view/25 |
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author | Ahmed N. Fayad |
author_facet | Ahmed N. Fayad |
author_sort | Ahmed N. Fayad |
collection | DOAJ |
description | In this study the clinical diagnosis of lumpy skin disease was depended. A total 1606 serum samples and skin biopsies collected from infected cows clinically with skin nodule of different size which change to a necrotic nodule or form a deep scab, from wide different areas and cities of Thi Qar , extended from June to November at 2016. ELISA were performed for identification of LSDV antibodies, LSDV isolation was carried out on CAM of ECE and Viral DNA was extracted from skin biopsy for PCR. All serum samples was show seropositivity against LSD. Isolation of LSD virus from skin biopsy was conducted on CAM and the isolated virus was identified by PCR. collection and processing of clinical samples, viral isolation and PCR assay, for LSDV are much sensitive and rapid diagnostic of LSD their importance in controlling the rapid spread of disease in Iraq. |
first_indexed | 2024-03-09T15:33:47Z |
format | Article |
id | doaj.art-178de72fe39e4fae9f4c4bcffb39fcb9 |
institution | Directory Open Access Journal |
issn | 1991-8690 2709-0256 |
language | English |
last_indexed | 2024-03-09T15:33:47Z |
publishDate | 2019-04-01 |
publisher | University of Thi-Qar |
record_format | Article |
series | مجلة علوم ذي قار |
spelling | doaj.art-178de72fe39e4fae9f4c4bcffb39fcb92023-11-26T09:41:44ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0163A study of Lumpy skin disease outbreak in Thi Qar ProvinceAhmed N. Fayad0Collage of Veterinary Medicine/University of Thi-QarIn this study the clinical diagnosis of lumpy skin disease was depended. A total 1606 serum samples and skin biopsies collected from infected cows clinically with skin nodule of different size which change to a necrotic nodule or form a deep scab, from wide different areas and cities of Thi Qar , extended from June to November at 2016. ELISA were performed for identification of LSDV antibodies, LSDV isolation was carried out on CAM of ECE and Viral DNA was extracted from skin biopsy for PCR. All serum samples was show seropositivity against LSD. Isolation of LSD virus from skin biopsy was conducted on CAM and the isolated virus was identified by PCR. collection and processing of clinical samples, viral isolation and PCR assay, for LSDV are much sensitive and rapid diagnostic of LSD their importance in controlling the rapid spread of disease in Iraq.https://mail.jsci.utq.edu.iq/index.php/main/article/view/25Lumpy skin diseaseELISAPCR |
spellingShingle | Ahmed N. Fayad A study of Lumpy skin disease outbreak in Thi Qar Province مجلة علوم ذي قار Lumpy skin disease ELISA PCR |
title | A study of Lumpy skin disease outbreak in Thi Qar Province |
title_full | A study of Lumpy skin disease outbreak in Thi Qar Province |
title_fullStr | A study of Lumpy skin disease outbreak in Thi Qar Province |
title_full_unstemmed | A study of Lumpy skin disease outbreak in Thi Qar Province |
title_short | A study of Lumpy skin disease outbreak in Thi Qar Province |
title_sort | study of lumpy skin disease outbreak in thi qar province |
topic | Lumpy skin disease ELISA PCR |
url | https://mail.jsci.utq.edu.iq/index.php/main/article/view/25 |
work_keys_str_mv | AT ahmednfayad astudyoflumpyskindiseaseoutbreakinthiqarprovince AT ahmednfayad studyoflumpyskindiseaseoutbreakinthiqarprovince |