Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae)
Pin nematodes (<i>Paratylenchus</i> spp.) currently comprise 132 species of polyphagous plant ectoparasites with at least seven species that are plant pathogenic emphasizing the need for correct identification to establish an appropriate management strategy. Sequences of highly conserved...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/4/343 |
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| author | Teresa Rosmaninho Manuel Mota Maria L. Inácio Jonathan D. Eisenback Carlos Gutiérrez-Gutiérrez |
| author_facet | Teresa Rosmaninho Manuel Mota Maria L. Inácio Jonathan D. Eisenback Carlos Gutiérrez-Gutiérrez |
| author_sort | Teresa Rosmaninho |
| collection | DOAJ |
| description | Pin nematodes (<i>Paratylenchus</i> spp.) currently comprise 132 species of polyphagous plant ectoparasites with at least seven species that are plant pathogenic emphasizing the need for correct identification to establish an appropriate management strategy. Sequences of highly conserved regions of ribosomal and mitochondrial RNA genes are a powerful species-level diagnostic tool within Tylenchulidae. A nematological survey was conducted from 2019 to 2021 in commercial vineyards distributed in four major wine-producing regions in the Central and South Portugal. Nine populations of <i>Paratylenchus</i> spp. were isolated from the rhizosphere of grapevines that were characterized from morphological data and molecular phylogenetic analysis using two rRNA genes (D2–D3 expansion segments of the 28S, and partial 18S) and a region partial of the COI mtRNA gene. Contrasting morphological hypotheses with molecular data provided rapid detection of six species, specifically <i>P</i>. <i>goodeyi</i>, <i>P. hamatus</i>, <i>P</i>. <i>pedrami</i>, <i>P</i>. <i>tenicaudatus</i>, <i>P</i>. <i>variabilis</i>, and <i>P</i>. <i>veruculatus</i>. Bayesian inference (BI) phylogenetic trees for these molecular markers established phylogenetic relationships underscore the importance of using genomic and molecular phylogenetic data for accurate pin nematode identification at the species level. To our knowledge, this is the first report of <i>Paratylenchus</i> spp. in Portugal, extending the geographical distribution of these species in the Mediterranean Basin, and the first record of <i>P</i>. <i>goodeyi</i>, <i>P. pedrami</i>, <i>P.</i><i>variabilis</i>, <i>P</i>. <i>veruculatus</i> and <i>P</i>. <i>tenicaudatus</i> parasitizing grapevine. |
| first_indexed | 2024-03-09T10:34:49Z |
| format | Article |
| id | doaj.art-178e0a334fa34079b3be9372aafe3d5e |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T10:34:49Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-178e0a334fa34079b3be9372aafe3d5e2023-12-01T21:00:49ZengMDPI AGHorticulturae2311-75242022-04-018434310.3390/horticulturae8040343Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae)Teresa Rosmaninho0Manuel Mota1Maria L. Inácio2Jonathan D. Eisenback3Carlos Gutiérrez-Gutiérrez4NemaLab/MED, Instituto Mediterrâneo para a Agricultura, Ambiente e Desenvolvimento, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Evora, PortugalNemaLab/MED, Instituto Mediterrâneo para a Agricultura, Ambiente e Desenvolvimento, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Evora, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, 2780-159 Oeiras, PortugalSchool of Plant and Environmental Science, Virginia Tech, Blacksburg, VA 24060, USANemaLab/MED, Instituto Mediterrâneo para a Agricultura, Ambiente e Desenvolvimento, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554 Evora, PortugalPin nematodes (<i>Paratylenchus</i> spp.) currently comprise 132 species of polyphagous plant ectoparasites with at least seven species that are plant pathogenic emphasizing the need for correct identification to establish an appropriate management strategy. Sequences of highly conserved regions of ribosomal and mitochondrial RNA genes are a powerful species-level diagnostic tool within Tylenchulidae. A nematological survey was conducted from 2019 to 2021 in commercial vineyards distributed in four major wine-producing regions in the Central and South Portugal. Nine populations of <i>Paratylenchus</i> spp. were isolated from the rhizosphere of grapevines that were characterized from morphological data and molecular phylogenetic analysis using two rRNA genes (D2–D3 expansion segments of the 28S, and partial 18S) and a region partial of the COI mtRNA gene. Contrasting morphological hypotheses with molecular data provided rapid detection of six species, specifically <i>P</i>. <i>goodeyi</i>, <i>P. hamatus</i>, <i>P</i>. <i>pedrami</i>, <i>P</i>. <i>tenicaudatus</i>, <i>P</i>. <i>variabilis</i>, and <i>P</i>. <i>veruculatus</i>. Bayesian inference (BI) phylogenetic trees for these molecular markers established phylogenetic relationships underscore the importance of using genomic and molecular phylogenetic data for accurate pin nematode identification at the species level. To our knowledge, this is the first report of <i>Paratylenchus</i> spp. in Portugal, extending the geographical distribution of these species in the Mediterranean Basin, and the first record of <i>P</i>. <i>goodeyi</i>, <i>P. pedrami</i>, <i>P.</i><i>variabilis</i>, <i>P</i>. <i>veruculatus</i> and <i>P</i>. <i>tenicaudatus</i> parasitizing grapevine.https://www.mdpi.com/2311-7524/8/4/343Bayesian inferenceD2–D3 expansion segments of large ribosomal subunit 28Scytochrome c oxidase subunit 1mitochondrial RNApartial small ribosomal subunitmorphology |
| spellingShingle | Teresa Rosmaninho Manuel Mota Maria L. Inácio Jonathan D. Eisenback Carlos Gutiérrez-Gutiérrez Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) Horticulturae Bayesian inference D2–D3 expansion segments of large ribosomal subunit 28S cytochrome c oxidase subunit 1 mitochondrial RNA partial small ribosomal subunit morphology |
| title | Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) |
| title_full | Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) |
| title_fullStr | Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) |
| title_full_unstemmed | Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) |
| title_short | Six First Reports of Pin Nematodes from Portugal, with an Update of the Systematics, Genetic Diversity, and Phylogeny of the Genus <i>Paratylenchus</i> (Nematoda: Tylenchulidae) |
| title_sort | six first reports of pin nematodes from portugal with an update of the systematics genetic diversity and phylogeny of the genus i paratylenchus i nematoda tylenchulidae |
| topic | Bayesian inference D2–D3 expansion segments of large ribosomal subunit 28S cytochrome c oxidase subunit 1 mitochondrial RNA partial small ribosomal subunit morphology |
| url | https://www.mdpi.com/2311-7524/8/4/343 |
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