Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications
Magnetic iron dust, a byproduct by many chemical industries that performs the reduction of nitro compounds to amine, was used for laccase immobilization. The characterization of magnetic iron dust was done by X-ray diffraction, Fourier-transform infrared, and dynamic light scattering. Biodegradable...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2016-10-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1177/0263617416661563 |
| _version_ | 1797283844160749568 |
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| author | Jagdish Shantilal Patel Darshan H Patel Rucha Desai Sunil Shah Piyush Chudasama Sachin Joshi Bhargav Patel |
| author_facet | Jagdish Shantilal Patel Darshan H Patel Rucha Desai Sunil Shah Piyush Chudasama Sachin Joshi Bhargav Patel |
| author_sort | Jagdish Shantilal Patel |
| collection | DOAJ |
| description | Magnetic iron dust, a byproduct by many chemical industries that performs the reduction of nitro compounds to amine, was used for laccase immobilization. The characterization of magnetic iron dust was done by X-ray diffraction, Fourier-transform infrared, and dynamic light scattering. Biodegradable polymer, chitosan, was coated on to the magnetic iron dust by reverse phase suspension method, which was confirmed by Fourier-transform infrared analysis. Immobilization of the laccase enzyme was done onto the chitosan-coated and non-coated magnetic iron dust. The immobilization was monitored by Fourier-transform infrared analysis. Binding efficiency, optimum pH, and optimum temperature for these immobilized laccases were investigated. X-ray diffraction pattern of magnetic iron dust confirmed presence of magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) with a particle size of 529.6 nm measured by dynamic light scattering. Laccase was immobilized on chitosan-coated and non-coated magnetic iron dust, monitored by Fourier-transform infrared spectra. Binding efficiency of the laccases was found to be 100% onto the coated and non-coated magnetic iron dust and their activity remained to be 63% and 82%, respectively, even after the 10th cycle of their use. The present results demonstrated the applicability of these immobilized laccase system in the industry in terms of their reusability and waste recycling. |
| first_indexed | 2024-03-07T17:37:41Z |
| format | Article |
| id | doaj.art-178f45d27e9b4cbf87b25ca61dae057a |
| institution | Directory Open Access Journal |
| issn | 0263-6174 2048-4038 |
| language | English |
| last_indexed | 2024-03-07T17:37:41Z |
| publishDate | 2016-10-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj.art-178f45d27e9b4cbf87b25ca61dae057a2024-03-02T16:26:39ZengSAGE PublicationsAdsorption Science & Technology0263-61742048-40382016-10-013410.1177/0263617416661563Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applicationsJagdish Shantilal PatelDarshan H PatelRucha DesaiSunil ShahPiyush ChudasamaSachin JoshiBhargav PatelMagnetic iron dust, a byproduct by many chemical industries that performs the reduction of nitro compounds to amine, was used for laccase immobilization. The characterization of magnetic iron dust was done by X-ray diffraction, Fourier-transform infrared, and dynamic light scattering. Biodegradable polymer, chitosan, was coated on to the magnetic iron dust by reverse phase suspension method, which was confirmed by Fourier-transform infrared analysis. Immobilization of the laccase enzyme was done onto the chitosan-coated and non-coated magnetic iron dust. The immobilization was monitored by Fourier-transform infrared analysis. Binding efficiency, optimum pH, and optimum temperature for these immobilized laccases were investigated. X-ray diffraction pattern of magnetic iron dust confirmed presence of magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) with a particle size of 529.6 nm measured by dynamic light scattering. Laccase was immobilized on chitosan-coated and non-coated magnetic iron dust, monitored by Fourier-transform infrared spectra. Binding efficiency of the laccases was found to be 100% onto the coated and non-coated magnetic iron dust and their activity remained to be 63% and 82%, respectively, even after the 10th cycle of their use. The present results demonstrated the applicability of these immobilized laccase system in the industry in terms of their reusability and waste recycling.https://doi.org/10.1177/0263617416661563 |
| spellingShingle | Jagdish Shantilal Patel Darshan H Patel Rucha Desai Sunil Shah Piyush Chudasama Sachin Joshi Bhargav Patel Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications Adsorption Science & Technology |
| title | Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| title_full | Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| title_fullStr | Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| title_full_unstemmed | Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| title_short | Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| title_sort | application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications |
| url | https://doi.org/10.1177/0263617416661563 |
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