Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage
The aim of this study was to compare in vitro the protective and antioxidant properties of lutein and astaxanthin on human primary corneal epithelial cells (HCE-F). To this purpose, HCE-F cells were irradiated with a blue-violet light lamp (415–420 nm) at different energies (20 to 80 J/cm<sup>...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/3/1268 |
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| author | Martina Cristaldi Carmelina Daniela Anfuso Giorgia Spampinato Dario Rusciano Gabriella Lupo |
| author_facet | Martina Cristaldi Carmelina Daniela Anfuso Giorgia Spampinato Dario Rusciano Gabriella Lupo |
| author_sort | Martina Cristaldi |
| collection | DOAJ |
| description | The aim of this study was to compare in vitro the protective and antioxidant properties of lutein and astaxanthin on human primary corneal epithelial cells (HCE-F). To this purpose, HCE-F cells were irradiated with a blue-violet light lamp (415–420 nm) at different energies (20 to 80 J/cm<sup>2</sup>). Lutein and astaxanthin (50 to 250 μM) were added to HCE-F right before blue-violet light irradiation at 50 J/cm<sup>2</sup>. Viability was evaluated by the CKK-8 assay while the production of reactive oxygen species (ROS) by the H2DCF-DA assay. Results have shown that the viability of HCE-F cells decreased at light energies from 20 J/cm<sup>2</sup> to 80 J/cm<sup>2</sup>, while ROS production increased at 50 and 80 J/cm<sup>2</sup>. The presence of lutein or astaxanthin protected the cells from phototoxicity, with lutein slightly more efficient than astaxanthin also on the blunting of ROS, prevention of apoptotic cell death and modulation of the Nrf-2 pathway. The association of lutein and astaxanthin did not give a significant advantage over the use of lutein alone. Taken together, these results suggest that the association of lutein and astaxanthin might be useful to protect cells of the ocular surface from short (lutein) and longer (astaxanthin) wavelengths, as these are the most damaging radiations hitting the eye from many different LED screens and solar light. |
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| last_indexed | 2024-03-10T00:13:42Z |
| publishDate | 2022-01-01 |
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| series | Applied Sciences |
| spelling | doaj.art-1796e933a2a443c783fe1c520e5f14ba2023-11-23T15:54:49ZengMDPI AGApplied Sciences2076-34172022-01-01123126810.3390/app12031268Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative DamageMartina Cristaldi0Carmelina Daniela Anfuso1Giorgia Spampinato2Dario Rusciano3Gabriella Lupo4Research Center, Sooft Italia SpA c/o Biologic Tower, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Biologic Tower, University of Catania, 95123 Catania, ItalyResearch Center, Sooft Italia SpA c/o Biologic Tower, University of Catania, 95123 Catania, ItalyResearch Center, Sooft Italia SpA c/o Biologic Tower, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Biologic Tower, University of Catania, 95123 Catania, ItalyThe aim of this study was to compare in vitro the protective and antioxidant properties of lutein and astaxanthin on human primary corneal epithelial cells (HCE-F). To this purpose, HCE-F cells were irradiated with a blue-violet light lamp (415–420 nm) at different energies (20 to 80 J/cm<sup>2</sup>). Lutein and astaxanthin (50 to 250 μM) were added to HCE-F right before blue-violet light irradiation at 50 J/cm<sup>2</sup>. Viability was evaluated by the CKK-8 assay while the production of reactive oxygen species (ROS) by the H2DCF-DA assay. Results have shown that the viability of HCE-F cells decreased at light energies from 20 J/cm<sup>2</sup> to 80 J/cm<sup>2</sup>, while ROS production increased at 50 and 80 J/cm<sup>2</sup>. The presence of lutein or astaxanthin protected the cells from phototoxicity, with lutein slightly more efficient than astaxanthin also on the blunting of ROS, prevention of apoptotic cell death and modulation of the Nrf-2 pathway. The association of lutein and astaxanthin did not give a significant advantage over the use of lutein alone. Taken together, these results suggest that the association of lutein and astaxanthin might be useful to protect cells of the ocular surface from short (lutein) and longer (astaxanthin) wavelengths, as these are the most damaging radiations hitting the eye from many different LED screens and solar light.https://www.mdpi.com/2076-3417/12/3/1268blue-violet lightluteinastaxanthinROScorneal cells |
| spellingShingle | Martina Cristaldi Carmelina Daniela Anfuso Giorgia Spampinato Dario Rusciano Gabriella Lupo Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage Applied Sciences blue-violet light lutein astaxanthin ROS corneal cells |
| title | Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage |
| title_full | Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage |
| title_fullStr | Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage |
| title_full_unstemmed | Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage |
| title_short | Comparative Efficiency of Lutein and Astaxanthin in the Protection of Human Corneal Epithelial Cells In Vitro from Blue-Violet Light Photo-Oxidative Damage |
| title_sort | comparative efficiency of lutein and astaxanthin in the protection of human corneal epithelial cells in vitro from blue violet light photo oxidative damage |
| topic | blue-violet light lutein astaxanthin ROS corneal cells |
| url | https://www.mdpi.com/2076-3417/12/3/1268 |
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