Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles
Abstract Background This study aims to assess the safety and efficacy of direct hemoperfusion using a new polymyxin B‐immobilized resin column (disposable endotoxin adsorber, KCEA) in an endotoxin/ lipopolysaccharide (LPS)‐induced sepsis model. Methods Eighteen beagles were randomized into 1 interve...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-04-01
|
| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12199 |
| _version_ | 1818274528467353600 |
|---|---|
| author | Yonggui Li Zhenggen Yang Jialiang Hu Zhennan Lin |
| author_facet | Yonggui Li Zhenggen Yang Jialiang Hu Zhennan Lin |
| author_sort | Yonggui Li |
| collection | DOAJ |
| description | Abstract Background This study aims to assess the safety and efficacy of direct hemoperfusion using a new polymyxin B‐immobilized resin column (disposable endotoxin adsorber, KCEA) in an endotoxin/ lipopolysaccharide (LPS)‐induced sepsis model. Methods Eighteen beagles were randomized into 1 intervention group (KCEA group, n = 6) and 2 control groups (sham group and model group, n = 6 each). Sepsis was induced by continuous intravenous application of 0.5 mg/kg body weight of endotoxin for 60 min. An extracorporeal hemoperfusion device made with KCEA for endotoxin adsorption was used. Model group beagles received standard treatment with fluids and vasoactive drugs, KCEA group beagles received standard treatment and direct hemoperfusion of KCEA for 2 h, and sham group beagles were treated with standard treatment and direct hemoperfusion of a sham column for 2 h. Results Good blood compatibility of KCEA was confirmed by assessing clinical parameters. Blood endotoxin peak levels in the KCEA group were significantly lower, resulting in a significant suppression of IL‐6, TNF‐α and procalcitonin, which improved mean arterial pressure and significantly lowered vasopressor demand, thereby protecting organ function and improving survival time and rate. In the KCEA group, MAP was significantly higher over 6 h than those recorded both in the sham group and model group. The 7‐day survival rates of the KCEA, sham and model groups were 50%, 0% and 0%, respectively. Conclusion KCEA hemoadsorption was effective at detoxifying circulatory endotoxin and inflammatory mediators and contributed to the decreased mortality rate in the sepsis beagles. |
| first_indexed | 2024-12-12T22:15:17Z |
| format | Article |
| id | doaj.art-17a2d52833a34bb29e191111c14351ff |
| institution | Directory Open Access Journal |
| issn | 2576-2095 |
| language | English |
| last_indexed | 2024-12-12T22:15:17Z |
| publishDate | 2022-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj.art-17a2d52833a34bb29e191111c14351ff2022-12-22T00:10:05ZengWileyAnimal Models and Experimental Medicine2576-20952022-04-015216117110.1002/ame2.12199Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beaglesYonggui Li0Zhenggen Yang1Jialiang Hu2Zhennan Lin3Research and Development Center Guangzhou Koncen Bioscience Co., Ltd. Guangzhou PR ChinaResearch and Development Center Guangzhou Koncen Bioscience Co., Ltd. Guangzhou PR ChinaResearch and Development Center Guangzhou Koncen Bioscience Co., Ltd. Guangzhou PR ChinaResearch and Development Center Guangzhou Koncen Bioscience Co., Ltd. Guangzhou PR ChinaAbstract Background This study aims to assess the safety and efficacy of direct hemoperfusion using a new polymyxin B‐immobilized resin column (disposable endotoxin adsorber, KCEA) in an endotoxin/ lipopolysaccharide (LPS)‐induced sepsis model. Methods Eighteen beagles were randomized into 1 intervention group (KCEA group, n = 6) and 2 control groups (sham group and model group, n = 6 each). Sepsis was induced by continuous intravenous application of 0.5 mg/kg body weight of endotoxin for 60 min. An extracorporeal hemoperfusion device made with KCEA for endotoxin adsorption was used. Model group beagles received standard treatment with fluids and vasoactive drugs, KCEA group beagles received standard treatment and direct hemoperfusion of KCEA for 2 h, and sham group beagles were treated with standard treatment and direct hemoperfusion of a sham column for 2 h. Results Good blood compatibility of KCEA was confirmed by assessing clinical parameters. Blood endotoxin peak levels in the KCEA group were significantly lower, resulting in a significant suppression of IL‐6, TNF‐α and procalcitonin, which improved mean arterial pressure and significantly lowered vasopressor demand, thereby protecting organ function and improving survival time and rate. In the KCEA group, MAP was significantly higher over 6 h than those recorded both in the sham group and model group. The 7‐day survival rates of the KCEA, sham and model groups were 50%, 0% and 0%, respectively. Conclusion KCEA hemoadsorption was effective at detoxifying circulatory endotoxin and inflammatory mediators and contributed to the decreased mortality rate in the sepsis beagles.https://doi.org/10.1002/ame2.12199endotoxinhemoadsorptionpolymyxin B sulfateresinsepsis |
| spellingShingle | Yonggui Li Zhenggen Yang Jialiang Hu Zhennan Lin Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles Animal Models and Experimental Medicine endotoxin hemoadsorption polymyxin B sulfate resin sepsis |
| title | Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles |
| title_full | Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles |
| title_fullStr | Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles |
| title_full_unstemmed | Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles |
| title_short | Preliminary study on the safety and efficacy of a new polymyxin B‐immoblized resin column in treatment of LPS‐induced sepsis beagles |
| title_sort | preliminary study on the safety and efficacy of a new polymyxin b immoblized resin column in treatment of lps induced sepsis beagles |
| topic | endotoxin hemoadsorption polymyxin B sulfate resin sepsis |
| url | https://doi.org/10.1002/ame2.12199 |
| work_keys_str_mv | AT yongguili preliminarystudyonthesafetyandefficacyofanewpolymyxinbimmoblizedresincolumnintreatmentoflpsinducedsepsisbeagles AT zhenggenyang preliminarystudyonthesafetyandefficacyofanewpolymyxinbimmoblizedresincolumnintreatmentoflpsinducedsepsisbeagles AT jialianghu preliminarystudyonthesafetyandefficacyofanewpolymyxinbimmoblizedresincolumnintreatmentoflpsinducedsepsisbeagles AT zhennanlin preliminarystudyonthesafetyandefficacyofanewpolymyxinbimmoblizedresincolumnintreatmentoflpsinducedsepsisbeagles |