The Genotoxicity of Vitamin C in vitro
The genotoxic effects of Vitamin C (ascorbic acid) on human lymphocytes in vitro were estimated by analyzing and identifying various chromosome abnormalities, in relation to the concentration of Vitamin C. Testing concentrations of Vitamin C induced different aberrations including the impairment of...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2008-05-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2969 |
| _version_ | 1797260805972951040 |
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| author | Hilada Nefić |
| author_facet | Hilada Nefić |
| author_sort | Hilada Nefić |
| collection | DOAJ |
| description |
The genotoxic effects of Vitamin C (ascorbic acid) on human lymphocytes in vitro were estimated by analyzing and identifying various chromosome abnormalities, in relation to the concentration of Vitamin C. Testing concentrations of Vitamin C induced different aberrations including the impairment of spindle function. The spindle disturbances can result in mitotic arrest, multipolar spindles and multipolar segregation, errors in chromosome segregation, formation of chromosome bridges and chromosome laggards. The most frequent irregularities were found in anaphase and telophase. A certain number of lymphocytes were arrested at anaphase or telophase (in colchicine-untreated cultures of human lymphocytes). Testing concentrations of ascorbic acid did not induce a significant increase in the number of aneuploid mitoses and were not clastogenic except at the highest concentration (1,000 μg/ml) in colchicine-treated cultures, and in colchicine-untreated cultures of human lymphocytes the pulverization of chromosome was observed. Vitamin C changed the mitotic index value of lymphocytes notably at the higher concentrations (250, 500 and 1,000 μg/ml).
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| first_indexed | 2024-04-24T23:31:10Z |
| format | Article |
| id | doaj.art-17aa537a56be49009a63a4f0bda120ec |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:31:10Z |
| publishDate | 2008-05-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-17aa537a56be49009a63a4f0bda120ec2024-03-15T14:38:27ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2008-05-018210.17305/bjbms.2008.2969571The Genotoxicity of Vitamin C in vitroHilada Nefić0Department of Biology, Faculty of Science, University of Sarajevo The genotoxic effects of Vitamin C (ascorbic acid) on human lymphocytes in vitro were estimated by analyzing and identifying various chromosome abnormalities, in relation to the concentration of Vitamin C. Testing concentrations of Vitamin C induced different aberrations including the impairment of spindle function. The spindle disturbances can result in mitotic arrest, multipolar spindles and multipolar segregation, errors in chromosome segregation, formation of chromosome bridges and chromosome laggards. The most frequent irregularities were found in anaphase and telophase. A certain number of lymphocytes were arrested at anaphase or telophase (in colchicine-untreated cultures of human lymphocytes). Testing concentrations of ascorbic acid did not induce a significant increase in the number of aneuploid mitoses and were not clastogenic except at the highest concentration (1,000 μg/ml) in colchicine-treated cultures, and in colchicine-untreated cultures of human lymphocytes the pulverization of chromosome was observed. Vitamin C changed the mitotic index value of lymphocytes notably at the higher concentrations (250, 500 and 1,000 μg/ml). https://www.bjbms.org/ojs/index.php/bjbms/article/view/2969Vitamin C (ascorbic acid)human lymphocyteschromosome abnormalities |
| spellingShingle | Hilada Nefić The Genotoxicity of Vitamin C in vitro Biomolecules & Biomedicine Vitamin C (ascorbic acid) human lymphocytes chromosome abnormalities |
| title | The Genotoxicity of Vitamin C in vitro |
| title_full | The Genotoxicity of Vitamin C in vitro |
| title_fullStr | The Genotoxicity of Vitamin C in vitro |
| title_full_unstemmed | The Genotoxicity of Vitamin C in vitro |
| title_short | The Genotoxicity of Vitamin C in vitro |
| title_sort | genotoxicity of vitamin c in vitro |
| topic | Vitamin C (ascorbic acid) human lymphocytes chromosome abnormalities |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2969 |
| work_keys_str_mv | AT hiladanefic thegenotoxicityofvitamincinvitro AT hiladanefic genotoxicityofvitamincinvitro |