Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>)
Gilthead sea bream (<i>Sparus aurata</i>) is one of the most important farmed Mediterranean fish species, and there is considerable interest for the development of suitable methods to assess its freshness. In the present work, gas chromatography–mass spectrometry-based metabolomics was e...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/4/464 |
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| author | Athanasios Mallouchos Theano Mikrou Chrysavgi Gardeli |
| author_facet | Athanasios Mallouchos Theano Mikrou Chrysavgi Gardeli |
| author_sort | Athanasios Mallouchos |
| collection | DOAJ |
| description | Gilthead sea bream (<i>Sparus aurata</i>) is one of the most important farmed Mediterranean fish species, and there is considerable interest for the development of suitable methods to assess its freshness. In the present work, gas chromatography–mass spectrometry-based metabolomics was employed to monitor the hydrophilic metabolites of sea bream during storage on ice for 19 days. Additionally, the quality changes were evaluated using two conventional methods: sensory evaluation according to European Union’s grading scheme and <i>K</i>-value, the most widely used chemical index of fish spoilage. With the application of chemometrics, the fish samples were successfully classified in the freshness categories, and a partial least squares regression model was built to predict <i>K</i>-value. A list of differential metabolites were found, which were distinguished according to their evolution profile as potential biomarkers of freshness and spoilage. Therefore, the results support the suitability of the proposed methodology to gain information on seafood quality. |
| first_indexed | 2024-03-10T20:35:32Z |
| format | Article |
| id | doaj.art-17ab375c0717404b86421fb2f743b81d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T20:35:32Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-17ab375c0717404b86421fb2f743b81d2023-11-19T21:04:05ZengMDPI AGFoods2304-81582020-04-019446410.3390/foods9040464Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>)Athanasios Mallouchos0Theano Mikrou1Chrysavgi Gardeli2Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceDepartment of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, GreeceGilthead sea bream (<i>Sparus aurata</i>) is one of the most important farmed Mediterranean fish species, and there is considerable interest for the development of suitable methods to assess its freshness. In the present work, gas chromatography–mass spectrometry-based metabolomics was employed to monitor the hydrophilic metabolites of sea bream during storage on ice for 19 days. Additionally, the quality changes were evaluated using two conventional methods: sensory evaluation according to European Union’s grading scheme and <i>K</i>-value, the most widely used chemical index of fish spoilage. With the application of chemometrics, the fish samples were successfully classified in the freshness categories, and a partial least squares regression model was built to predict <i>K</i>-value. A list of differential metabolites were found, which were distinguished according to their evolution profile as potential biomarkers of freshness and spoilage. Therefore, the results support the suitability of the proposed methodology to gain information on seafood quality.https://www.mdpi.com/2304-8158/9/4/464sea breamfishspoilagemetabolomicsmultivariate analysisbiomarkers |
| spellingShingle | Athanasios Mallouchos Theano Mikrou Chrysavgi Gardeli Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) Foods sea bream fish spoilage metabolomics multivariate analysis biomarkers |
| title | Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_full | Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_fullStr | Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_full_unstemmed | Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_short | Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (<i>Sparus aurata</i>) |
| title_sort | gas chromatography mass spectrometry based metabolite profiling for the assessment of freshness in gilthead sea bream i sparus aurata i |
| topic | sea bream fish spoilage metabolomics multivariate analysis biomarkers |
| url | https://www.mdpi.com/2304-8158/9/4/464 |
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