A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity
Abstract Cerium oxide nanoparticles (CNPs) are novel synthetic antioxidant agents proposed for treating oxidative stress-related diseases. The synthesis of high-quality CNPs for biomedical applications remains a challenging task. A major concern for a safe use of CNPs as pharmacological agents is th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04098-6 |
| _version_ | 1818851106617294848 |
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| author | Fanny Caputo Marta Mameli Andrzej Sienkiewicz Silvia Licoccia Francesco Stellacci Lina Ghibelli Enrico Traversa |
| author_facet | Fanny Caputo Marta Mameli Andrzej Sienkiewicz Silvia Licoccia Francesco Stellacci Lina Ghibelli Enrico Traversa |
| author_sort | Fanny Caputo |
| collection | DOAJ |
| description | Abstract Cerium oxide nanoparticles (CNPs) are novel synthetic antioxidant agents proposed for treating oxidative stress-related diseases. The synthesis of high-quality CNPs for biomedical applications remains a challenging task. A major concern for a safe use of CNPs as pharmacological agents is their tendency to agglomerate. Herein we present a simple direct precipitation approach, exploiting ethylene glycol as synthesis co-factor, to synthesize at room temperature nanocrystalline sub-10 nm CNPs, followed by a surface silanization approach to improve nanoparticle dispersibility in biological fluids. CNPs were characterized using transmission electron microscopy (TEM) observations, X-ray diffraction (XRD) analysis, thermogravimetric analysis (TGA), Fourier-transform infrared (FT-IR) spectroscopy, proton nuclear magnetic resonance (1H-NMR) spectroscopy, dynamic light scattering (DLS) and zeta potential measurements. CNP redox activity was studied in abiotic systems using electron spin resonance (ESR) measurements, and in vitro on human cell models. In-situ silanization improved CNP colloidal stability, in comparison with non-functionalized particles, and allowed at the same time improving their original biological activity, yielding thus functionalized CNPs suitable for biomedical applications. |
| first_indexed | 2024-12-19T06:59:45Z |
| format | Article |
| id | doaj.art-17ac40f4c50e4ad2b1e9acb342aa0e45 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T06:59:45Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-17ac40f4c50e4ad2b1e9acb342aa0e452022-12-21T20:31:27ZengNature PortfolioScientific Reports2045-23222017-07-017111310.1038/s41598-017-04098-6A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activityFanny Caputo0Marta Mameli1Andrzej Sienkiewicz2Silvia Licoccia3Francesco Stellacci4Lina Ghibelli5Enrico Traversa6Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor VergataInstitute of Materials, École Polytechnique Fédérale de LausanneInstitute of Physics, École Polytechnique Fédérale de LausanneDipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor VergataInstitute of Materials, École Polytechnique Fédérale de LausanneDipartimento di Biologia, Università di Roma Tor VergataDipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor VergataAbstract Cerium oxide nanoparticles (CNPs) are novel synthetic antioxidant agents proposed for treating oxidative stress-related diseases. The synthesis of high-quality CNPs for biomedical applications remains a challenging task. A major concern for a safe use of CNPs as pharmacological agents is their tendency to agglomerate. Herein we present a simple direct precipitation approach, exploiting ethylene glycol as synthesis co-factor, to synthesize at room temperature nanocrystalline sub-10 nm CNPs, followed by a surface silanization approach to improve nanoparticle dispersibility in biological fluids. CNPs were characterized using transmission electron microscopy (TEM) observations, X-ray diffraction (XRD) analysis, thermogravimetric analysis (TGA), Fourier-transform infrared (FT-IR) spectroscopy, proton nuclear magnetic resonance (1H-NMR) spectroscopy, dynamic light scattering (DLS) and zeta potential measurements. CNP redox activity was studied in abiotic systems using electron spin resonance (ESR) measurements, and in vitro on human cell models. In-situ silanization improved CNP colloidal stability, in comparison with non-functionalized particles, and allowed at the same time improving their original biological activity, yielding thus functionalized CNPs suitable for biomedical applications.https://doi.org/10.1038/s41598-017-04098-6 |
| spellingShingle | Fanny Caputo Marta Mameli Andrzej Sienkiewicz Silvia Licoccia Francesco Stellacci Lina Ghibelli Enrico Traversa A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity Scientific Reports |
| title | A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| title_full | A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| title_fullStr | A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| title_full_unstemmed | A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| title_short | A novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| title_sort | novel synthetic approach of cerium oxide nanoparticles with improved biomedical activity |
| url | https://doi.org/10.1038/s41598-017-04098-6 |
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