Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells
Some metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing to tissue growth and regeneration. To address...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/3/253 |
| _version_ | 1797608744275673088 |
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| author | Pavel Rossner Tereza Cervena Barbora Echalar Katerina Palacka Alena Milcova Zuzana Novakova Michal Sima Zuzana Simova Jolana Vankova Vladimir Holan |
| author_facet | Pavel Rossner Tereza Cervena Barbora Echalar Katerina Palacka Alena Milcova Zuzana Novakova Michal Sima Zuzana Simova Jolana Vankova Vladimir Holan |
| author_sort | Pavel Rossner |
| collection | DOAJ |
| description | Some metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing to tissue growth and regeneration. To address these issues, we investigated the toxic effects of selected NP (Ag, ZnO, and CuO) in mouse MSC. MSC were treated with various doses of NP for 4 h, 24 h, and 48 h and multiple endpoints were analyzed. Reactive oxygen species were generated after 48 h CuO NP exposure. Lipid peroxidation was induced after 4 h and 24 h treatment, regardless of NP and/or tested dose. DNA fragmentation and oxidation induced by Ag NP showed dose responses for all the periods. For other NP, the effects were observed for shorter exposure times. The impact on the frequency of micronuclei was weak. All the tested NP increased the sensitivity of MSC to apoptosis. The cell cycle was most affected after 24 h, particularly for Ag NP treatment. In summary, the tested NP induced numerous adverse changes in MSC. These results should be taken into consideration when planning the use of NP in medical applications where MSC are involved. |
| first_indexed | 2024-03-11T05:48:58Z |
| format | Article |
| id | doaj.art-17afed162c0048d89cc8d52dfe12f21a |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T05:48:58Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-17afed162c0048d89cc8d52dfe12f21a2023-11-17T14:13:16ZengMDPI AGToxics2305-63042023-03-0111325310.3390/toxics11030253Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem CellsPavel Rossner0Tereza Cervena1Barbora Echalar2Katerina Palacka3Alena Milcova4Zuzana Novakova5Michal Sima6Zuzana Simova7Jolana Vankova8Vladimir Holan9Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicDepartment of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00 Prague, Czech RepublicSome metal nanoparticles (NP) are characterized by antimicrobial properties with the potential to be used as alternative antibiotics. However, NP may negatively impact human organism, including mesenchymal stem cells (MSC), a cell population contributing to tissue growth and regeneration. To address these issues, we investigated the toxic effects of selected NP (Ag, ZnO, and CuO) in mouse MSC. MSC were treated with various doses of NP for 4 h, 24 h, and 48 h and multiple endpoints were analyzed. Reactive oxygen species were generated after 48 h CuO NP exposure. Lipid peroxidation was induced after 4 h and 24 h treatment, regardless of NP and/or tested dose. DNA fragmentation and oxidation induced by Ag NP showed dose responses for all the periods. For other NP, the effects were observed for shorter exposure times. The impact on the frequency of micronuclei was weak. All the tested NP increased the sensitivity of MSC to apoptosis. The cell cycle was most affected after 24 h, particularly for Ag NP treatment. In summary, the tested NP induced numerous adverse changes in MSC. These results should be taken into consideration when planning the use of NP in medical applications where MSC are involved.https://www.mdpi.com/2305-6304/11/3/253metal nanoparticlesantimicrobial propertiesmesenchymal stem cellstoxicity |
| spellingShingle | Pavel Rossner Tereza Cervena Barbora Echalar Katerina Palacka Alena Milcova Zuzana Novakova Michal Sima Zuzana Simova Jolana Vankova Vladimir Holan Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells Toxics metal nanoparticles antimicrobial properties mesenchymal stem cells toxicity |
| title | Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells |
| title_full | Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells |
| title_fullStr | Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells |
| title_full_unstemmed | Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells |
| title_short | Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem Cells |
| title_sort | metal nanoparticles with antimicrobial properties the toxicity response in mouse mesenchymal stem cells |
| topic | metal nanoparticles antimicrobial properties mesenchymal stem cells toxicity |
| url | https://www.mdpi.com/2305-6304/11/3/253 |
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