Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate
An amperometric benzoic acid-sensing inhibitor biosensor was prepared by immobilizing mushroom (Agaricus bisporus) tissue homogenate on a Clark-type oxygen electrode. The effects of the quantity of mushroom tissue homogenate, the quantity of gelatin and the effect of the crosslinking agent glutarald...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2005-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/162923 |
| _version_ | 1827609960767291392 |
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| author | Mustafa Kemal Sezgintürk Tüge Göktug Erhan Dinçkaya |
| author_facet | Mustafa Kemal Sezgintürk Tüge Göktug Erhan Dinçkaya |
| author_sort | Mustafa Kemal Sezgintürk |
| collection | DOAJ |
| description | An amperometric benzoic acid-sensing inhibitor biosensor was prepared by immobilizing mushroom (Agaricus bisporus) tissue homogenate on a Clark-type oxygen electrode. The effects of the quantity of mushroom tissue homogenate, the quantity of gelatin and the effect of the crosslinking agent glutaraldehyde percent on the biosensor were studied. The optimum concentration of phenol used as substrate was 200 μM. The bioanalytical properties of the proposed biosensor, such as dependence of the biosensor response on the pH value and the temperature, were investigated. The biosensor responded linearly to benzoic acid in a concentration range of 25–100 μM. Standard deviation (s.d.) was ±0.49 μM for 7 successive determinations at a concentration of 75 μM. The inhibitor biosensor based on mushroom tissue homogenate was applied for the determination of benzoic acid in fizzy lemonade, some fruits and groundwater samples. Results were compared to those obtained using AOAC method, showing a good agreement. |
| first_indexed | 2024-03-09T07:41:54Z |
| format | Article |
| id | doaj.art-17b24a6cf5fd42a5a0cd0a784a991d8e |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:41:54Z |
| publishDate | 2005-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-17b24a6cf5fd42a5a0cd0a784a991d8e2023-12-03T04:22:00ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062005-01-01434329334Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue HomogenateMustafa Kemal Sezgintürk0Tüge Göktug1Erhan Dinçkaya2Ege University, Faculty of Science, Biochemistry Department 35100, Bornova-Izmir, TurkeyEge University, Faculty of Science, Biochemistry Department 35100, Bornova-Izmir, TurkeyEge University, Faculty of Science, Biochemistry Department 35100, Bornova-Izmir, TurkeyAn amperometric benzoic acid-sensing inhibitor biosensor was prepared by immobilizing mushroom (Agaricus bisporus) tissue homogenate on a Clark-type oxygen electrode. The effects of the quantity of mushroom tissue homogenate, the quantity of gelatin and the effect of the crosslinking agent glutaraldehyde percent on the biosensor were studied. The optimum concentration of phenol used as substrate was 200 μM. The bioanalytical properties of the proposed biosensor, such as dependence of the biosensor response on the pH value and the temperature, were investigated. The biosensor responded linearly to benzoic acid in a concentration range of 25–100 μM. Standard deviation (s.d.) was ±0.49 μM for 7 successive determinations at a concentration of 75 μM. The inhibitor biosensor based on mushroom tissue homogenate was applied for the determination of benzoic acid in fizzy lemonade, some fruits and groundwater samples. Results were compared to those obtained using AOAC method, showing a good agreement.http://hrcak.srce.hr/file/162923benzoic acidinhibitor biosensorspolyphenol oxidasesfood additivesClark--type oxygen electrode |
| spellingShingle | Mustafa Kemal Sezgintürk Tüge Göktug Erhan Dinçkaya Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate Food Technology and Biotechnology benzoic acid inhibitor biosensors polyphenol oxidases food additives Clark--type oxygen electrode |
| title | Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate |
| title_full | Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate |
| title_fullStr | Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate |
| title_full_unstemmed | Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate |
| title_short | Detection of Benzoic Acid by an Amperometric Inhibitor Biosensor Based on Mushroom Tissue Homogenate |
| title_sort | detection of benzoic acid by an amperometric inhibitor biosensor based on mushroom tissue homogenate |
| topic | benzoic acid inhibitor biosensors polyphenol oxidases food additives Clark--type oxygen electrode |
| url | http://hrcak.srce.hr/file/162923 |
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